Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice

Detalhes bibliográficos
Autor(a) principal: Barbosa, Aline da Silva
Data de Publicação: 2021
Outros Autores: Temple, Mayani Costa Ribeiro, Varela, Everton Luiz Pompeu, Gomes, Antonio Rafael Quadros, Silveira, Edvaldo Lima, Carvalho, Eliete Pereira de, Dolabela, Maria Fani, Percario, Sandro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/11805
Resumo: Nitric oxide (NO) is an important mediator molecule in inflammatory processes, but its role in the pathophysiology of malaria is still uncertain. To investigate the NO synthesis inhibition on the oxidative changes induced by Plasmodium berghei infection in mice, malaria was induced in 150 animals, of which 75 animals were treated with the NO inhibitor L-NAME; the remaining animals were sham controls. All animals underwent euthanasia 1, 5, 10, 15, or 20 days after infection for the collection of lungs, brain, and blood. Parasitemia was determined, and the survival of the animals was evaluated. Tissue samples were assayed for nitrites and nitrates (NN), thiobarbituric acid reactive substances (TBARS), and total Trolox equivalent antioxidant capacity (TEAC). A histopathological study was performed. Mortality rates in the L-NAME group were always higher than those in the controls. In the brain, NN was lower in the L-NAME group. Parasitemia and its progression rate were greater in the control group. By the 5th day of infection, mice treated with L-NAME showed cerebral edema and interstitial pneumonia of greater intensity than controls. In conclusion, the anti-inflammatory and hemodynamic effects of NO surpass its pro-oxidant role in murine malaria.
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spelling Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected miceLa inhibición de la síntesis de óxido nítrico promueve el aumento de la mortalidad a pesar de la reducción de la parasitemia en ratones infectados por Plasmodium bergheiInibição da síntese de óxido nítrico promove aumento da mortalidade, apesar da redução da parasitemia em camundongos infectados pelo Plasmodium bergheiÓxido nítricoMaláriaÓxido nítrico sintaseEstresse oxidativo.Óxido nítricoMalariaÓxido nítrico sintasaEstrés oxidativo.Nitric oxideMalariaNitric oxide synthaseOxidative stress.Nitric oxide (NO) is an important mediator molecule in inflammatory processes, but its role in the pathophysiology of malaria is still uncertain. To investigate the NO synthesis inhibition on the oxidative changes induced by Plasmodium berghei infection in mice, malaria was induced in 150 animals, of which 75 animals were treated with the NO inhibitor L-NAME; the remaining animals were sham controls. All animals underwent euthanasia 1, 5, 10, 15, or 20 days after infection for the collection of lungs, brain, and blood. Parasitemia was determined, and the survival of the animals was evaluated. Tissue samples were assayed for nitrites and nitrates (NN), thiobarbituric acid reactive substances (TBARS), and total Trolox equivalent antioxidant capacity (TEAC). A histopathological study was performed. Mortality rates in the L-NAME group were always higher than those in the controls. In the brain, NN was lower in the L-NAME group. Parasitemia and its progression rate were greater in the control group. By the 5th day of infection, mice treated with L-NAME showed cerebral edema and interstitial pneumonia of greater intensity than controls. In conclusion, the anti-inflammatory and hemodynamic effects of NO surpass its pro-oxidant role in murine malaria.El óxido nítrico (NO) es una molécula mediadora importante en los procesos inflamatorios, pero su papel en la fisiopatología de la malaria sigue siendo incierto. Para investigar la inhibición de la síntesis de NO en los cambios oxidativos inducidos por la infección por Plasmodium berghei en ratones, la malaria se indujo en 150 animales, de los cuales 75 animales fueron tratados con el inhibidor de NO L-NAME; los animales restantes eran controles sham. Todos los animales se sometieron a eutanasia 1, 5, 10, 15 o 20 días después de la infección para la recolección de pulmones, cerebro y sangre. Se determinó la parasitemia, y se evaluó la supervivencia de los animales. Se ensayaron muestras de tejido para nitritos y nitratos (NN), sustancias reactivas de ácido tiobarbitúrico (TBARS) y capacidad antioxidante total equivalente a Trolox (TEAC). Se realizó un estudio histopatológico. Las tasas de mortalidad en el grupo L-NAME siempre fueron más altas que las de los controles. En el cerebro, NN era más baja en el grupo L-NAME. Parasitemia y su tasa de progresión fueron mayores en el grupo de control. Para el 5o día de infección, los ratones tratados con L-NAME mostraron edema cerebral y neumonía intersticial de mayor intensidad que los controles. En conclusión, los efectos antiinflamatorios y hemodinámicos de NO superan su papel pro-oxidante en la malaria murina.O óxido nítrico (NO) é uma importante molécula mediadora em processos inflamatórios, mas seu papel na fisiopatologia da malária ainda é incerto. Para investigar a inibição da síntese NO sobre as alterações oxidativas induzidas pela infecção por Plasmodium berghei em camundongos, a malária foi induzida em 150 animais, dos quais 75 animais foram tratados com o inibidor de NO L-NAME; os demais animais eram controles sham. Todos os animais foram submetidos à eutanásia 1, 5, 10, 15 ou 20 dias após a infecção para coleta de pulmões, cérebro e sangue. A parasitemia foi determinada, e a sobrevivência dos animais foi avaliada. As amostras de tecido foram avaliadas para nitritos e nitratos (NN), substâncias reativas de ácido tiobarbitúrico (TBARS) e capacidade antioxidante equivalente trolox total (TEAC). Foi realizado um estudo histopatológico. As taxas de mortalidade no grupo L-NAME sempre foram maiores do que as dos controles. No cérebro, NN era menor no grupo L-NAME. A parasitemia e sua taxa de progressão foram maiores no grupo controle. No 5th dia de infecção, os camundongos tratados com L-NAME apresentaram edema cerebral e pneumonia intersticial de maior intensidade do que os controles. Em conclusão, os efeitos anti-inflamatórios e hemodinâmicos de NO superam seu papel pró-oxidante na malária murina.Research, Society and Development2021-01-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1180510.33448/rsd-v10i1.11805Research, Society and Development; Vol. 10 No. 1; e27810111805Research, Society and Development; Vol. 10 Núm. 1; e27810111805Research, Society and Development; v. 10 n. 1; e278101118052525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/11805/10511Copyright (c) 2021 Aline da Silva Barbosa; Mayani Costa Ribeiro Temple; Everton Luiz Pompeu Varela; Antonio Rafael Quadros Gomes; Edvaldo Lima Silveira; Eliete Pereira de Carvalho; Maria Fani Dolabela; Sandro Percariohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBarbosa, Aline da Silva Temple, Mayani Costa Ribeiro Varela, Everton Luiz Pompeu Gomes, Antonio Rafael Quadros Silveira, Edvaldo Lima Carvalho, Eliete Pereira de Dolabela, Maria Fani Percario, Sandro2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11805Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:32.348631Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
La inhibición de la síntesis de óxido nítrico promueve el aumento de la mortalidad a pesar de la reducción de la parasitemia en ratones infectados por Plasmodium berghei
Inibição da síntese de óxido nítrico promove aumento da mortalidade, apesar da redução da parasitemia em camundongos infectados pelo Plasmodium berghei
title Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
spellingShingle Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
Barbosa, Aline da Silva
Óxido nítrico
Malária
Óxido nítrico sintase
Estresse oxidativo.
Óxido nítrico
Malaria
Óxido nítrico sintasa
Estrés oxidativo.
Nitric oxide
Malaria
Nitric oxide synthase
Oxidative stress.
title_short Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
title_full Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
title_fullStr Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
title_full_unstemmed Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
title_sort Inhibition of nitric oxide synthesis promotes increased mortality despite the reduction of parasitemia in Plasmodium berghei-infected mice
author Barbosa, Aline da Silva
author_facet Barbosa, Aline da Silva
Temple, Mayani Costa Ribeiro
Varela, Everton Luiz Pompeu
Gomes, Antonio Rafael Quadros
Silveira, Edvaldo Lima
Carvalho, Eliete Pereira de
Dolabela, Maria Fani
Percario, Sandro
author_role author
author2 Temple, Mayani Costa Ribeiro
Varela, Everton Luiz Pompeu
Gomes, Antonio Rafael Quadros
Silveira, Edvaldo Lima
Carvalho, Eliete Pereira de
Dolabela, Maria Fani
Percario, Sandro
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Aline da Silva
Temple, Mayani Costa Ribeiro
Varela, Everton Luiz Pompeu
Gomes, Antonio Rafael Quadros
Silveira, Edvaldo Lima
Carvalho, Eliete Pereira de
Dolabela, Maria Fani
Percario, Sandro
dc.subject.por.fl_str_mv Óxido nítrico
Malária
Óxido nítrico sintase
Estresse oxidativo.
Óxido nítrico
Malaria
Óxido nítrico sintasa
Estrés oxidativo.
Nitric oxide
Malaria
Nitric oxide synthase
Oxidative stress.
topic Óxido nítrico
Malária
Óxido nítrico sintase
Estresse oxidativo.
Óxido nítrico
Malaria
Óxido nítrico sintasa
Estrés oxidativo.
Nitric oxide
Malaria
Nitric oxide synthase
Oxidative stress.
description Nitric oxide (NO) is an important mediator molecule in inflammatory processes, but its role in the pathophysiology of malaria is still uncertain. To investigate the NO synthesis inhibition on the oxidative changes induced by Plasmodium berghei infection in mice, malaria was induced in 150 animals, of which 75 animals were treated with the NO inhibitor L-NAME; the remaining animals were sham controls. All animals underwent euthanasia 1, 5, 10, 15, or 20 days after infection for the collection of lungs, brain, and blood. Parasitemia was determined, and the survival of the animals was evaluated. Tissue samples were assayed for nitrites and nitrates (NN), thiobarbituric acid reactive substances (TBARS), and total Trolox equivalent antioxidant capacity (TEAC). A histopathological study was performed. Mortality rates in the L-NAME group were always higher than those in the controls. In the brain, NN was lower in the L-NAME group. Parasitemia and its progression rate were greater in the control group. By the 5th day of infection, mice treated with L-NAME showed cerebral edema and interstitial pneumonia of greater intensity than controls. In conclusion, the anti-inflammatory and hemodynamic effects of NO surpass its pro-oxidant role in murine malaria.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/11805
10.33448/rsd-v10i1.11805
url https://rsdjournal.org/index.php/rsd/article/view/11805
identifier_str_mv 10.33448/rsd-v10i1.11805
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/11805/10511
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 1; e27810111805
Research, Society and Development; Vol. 10 Núm. 1; e27810111805
Research, Society and Development; v. 10 n. 1; e27810111805
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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