Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation

Detalhes bibliográficos
Autor(a) principal: Rocha, Flaviana Soares
Data de Publicação: 2020
Outros Autores: Rezende , Layra Gabriella Pereira de, Reis , Danyella Carolyna Soares dos, Rabelo, Gustavo Davi, Filice, Letícia de Souza Castro, Zanetta-Barbosa, Darceny, Dechichi, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/9948
Resumo: The present study aimed to evaluate the effects of HBO on bone repair after ionizing radiation, by using histomorphometry, computed tomography and polarization microscopy. Twenty male Wistar rats were used. One radiation dose (30 Gy) was administered on the left leg in all animals, and after 30 days, bone defects were created in both femurs. Then, 10 animals received daily HBO sessions (2.5 ATA for 90 minutes), and all animals were euthanized 5 or 7 days after surgery. The femurs were separated into 4 groups (n=5) for each euthanasia time interval: Control (Right femur: Non-irradiated and Non-HBO), RXT (Left femur: Irradiated and Non-HBO), HBO (Right femur: Non-irradiated with HBO), RXT+HBO (Left femur: Irradiated with HBO). The bone cortical and defects were evaluated. Data were analyzed using the Kolmogorov-Smirnov tests, unpaired t-test, and ANOVA with Bonferroni correction. Higher HU values and new bone formation were observed in Control and HBO groups, with improved repair. Group HBO showed predominant Yellow/Orange/Red birefringence of collagen in the defect area. HBO improved bone repair in physiological conditions, with increased blood vessels and bone formation. However, it was not efficient in improving repair and collagen orientation in bone after high doses of ionizing radiation.
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spelling Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen OrientationInfluencia de la terapia de oxígeno hiperbárico en la reparación ósea y la orientación del colágeno después de la radioterapiaInfluência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapiaIonizing radiationHyperbaric oxygenationOsteogenesisCollagen.Radiación ionizanteOxigenación hiperbáricaOsteogénesisColágeno.Radiação ionizanteOxigenação hiperbáricaOsteogêneseColágeno.The present study aimed to evaluate the effects of HBO on bone repair after ionizing radiation, by using histomorphometry, computed tomography and polarization microscopy. Twenty male Wistar rats were used. One radiation dose (30 Gy) was administered on the left leg in all animals, and after 30 days, bone defects were created in both femurs. Then, 10 animals received daily HBO sessions (2.5 ATA for 90 minutes), and all animals were euthanized 5 or 7 days after surgery. The femurs were separated into 4 groups (n=5) for each euthanasia time interval: Control (Right femur: Non-irradiated and Non-HBO), RXT (Left femur: Irradiated and Non-HBO), HBO (Right femur: Non-irradiated with HBO), RXT+HBO (Left femur: Irradiated with HBO). The bone cortical and defects were evaluated. Data were analyzed using the Kolmogorov-Smirnov tests, unpaired t-test, and ANOVA with Bonferroni correction. Higher HU values and new bone formation were observed in Control and HBO groups, with improved repair. Group HBO showed predominant Yellow/Orange/Red birefringence of collagen in the defect area. HBO improved bone repair in physiological conditions, with increased blood vessels and bone formation. However, it was not efficient in improving repair and collagen orientation in bone after high doses of ionizing radiation.El presente estudio tuvo como objetivo evaluar los efectos de la HBO en la reparación ósea después de la radiación ionizante, mediante el uso de histomorfometría, tomografía computarizada y microscopía de polarización. Se utilizaron veinte ratas Wistar macho. Se administró una dosis de radiación (30 Gy) en la pierna izquierda en todos los animales y, después de 30 días, se crearon defectos óseos en ambos fémures. Luego, 10 animales recibieron sesiones diarias de HBO (2,5 ATA durante 90 minutos), y todos los animales fueron sacrificados 5 o 7 días después de la cirugía. Los fémures se separaron en 4 grupos (n = 5) para cada intervalo de tiempo de eutanasia: Control (fémur derecho: no irradiado y no HBO), RXT (fémur izquierdo: irradiado y no HBO), HBO (fémur derecho: no HBO) -irradiado con HBO), RXT + HBO (fémur izquierdo: irradiado con HBO). Se evaluaron la cortical ósea y los defectos. Los datos se analizaron mediante las pruebas de Kolmogorov-Smirnov, la prueba t para datos no apareados y ANOVA con corrección de Bonferroni. Se observaron valores más altos de HU y formación de hueso nuevo en los grupos Control y HBO, con una mejor reparación. El grupo HBO mostró una birrefringencia predominante de colágeno amarillo / naranja / rojo en el área del defecto. HBO mejoró la reparación ósea en condiciones fisiológicas, con aumento de los vasos sanguíneos y la formación de hueso. Sin embargo, no fue eficaz para mejorar la reparación y la orientación del colágeno en el hueso después de altas dosis de radiación ionizante.O presente estudo teve como objetivo avaliar os efeitos da HBO no reparo ósseo após radiação ionizante, por meio da histomorfometria, tomografia computadorizada e microscopia de polarização. Vinte ratos Wistar machos foram usados. Uma dose de radiação (30 Gy) foi administrada na perna esquerda em todos os animais, e após 30 dias, defeitos ósseos foram criados em ambos os fêmures. Em seguida, 10 animais receberam sessões diárias de HBO (2,5 ATA por 90 minutos), e todos os animais foram sacrificados 5 ou 7 dias após a cirurgia. Os fêmures foram separados em 4 grupos (n = 5) para cada intervalo de tempo de eutanásia: Controle (fêmur direito: não irradiado e não HBO), RXT (fêmur esquerdo: irradiado e não HBO), HBO (fêmur direito: não-irradiado com HBO), RXT + HBO (fémur esquerdo: irradiado com HBO). A cortical óssea e os defeitos foram avaliados. Os dados foram analisados por meio dos testes de Kolmogorov-Smirnov, teste t não pareado e ANOVA com correção de Bonferroni. Valores maiores de HU e neoformação óssea foram observados nos grupos Controle e HBO, com melhora do reparo. O grupo HBO apresentou birrefringência de colágeno predominantemente amarela/laranja/vermelha na área do defeito. A HBO melhorou a reparação óssea em condições fisiológicas, com aumento dos vasos sanguíneos e formação óssea. No entanto, não foi eficiente em melhorar o reparo e a orientação do colágeno no osso após altas doses de radiação ionizante.Research, Society and Development2020-11-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/994810.33448/rsd-v9i11.9948Research, Society and Development; Vol. 9 No. 11; e4339119948Research, Society and Development; Vol. 9 Núm. 11; e4339119948Research, Society and Development; v. 9 n. 11; e43391199482525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9948/8965Copyright (c) 2020 Flaviana Soares Rocha; Layra Gabriella Pereira de Rezende ; Danyella Carolyna Soares dos Reis ; Gustavo Davi Rabelo; Letícia de Souza Castro Filice; Darceny Zanetta-Barbosa; Paula Dechichihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRocha, Flaviana SoaresRezende , Layra Gabriella Pereira de Reis , Danyella Carolyna Soares dos Rabelo, Gustavo DaviFilice, Letícia de Souza Castro Zanetta-Barbosa, DarcenyDechichi, Paula2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/9948Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:07.387370Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
Influencia de la terapia de oxígeno hiperbárico en la reparación ósea y la orientación del colágeno después de la radioterapia
Influência da oxigenação hiperbárica no reparo ósseo e organização do colágeno após radioterapia
title Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
spellingShingle Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
Rocha, Flaviana Soares
Ionizing radiation
Hyperbaric oxygenation
Osteogenesis
Collagen.
Radiación ionizante
Oxigenación hiperbárica
Osteogénesis
Colágeno.
Radiação ionizante
Oxigenação hiperbárica
Osteogênese
Colágeno.
title_short Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
title_full Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
title_fullStr Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
title_full_unstemmed Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
title_sort Hyperbaric Oxygen Therapy Influence on Irradiated Bone Repair and Collagen Orientation
author Rocha, Flaviana Soares
author_facet Rocha, Flaviana Soares
Rezende , Layra Gabriella Pereira de
Reis , Danyella Carolyna Soares dos
Rabelo, Gustavo Davi
Filice, Letícia de Souza Castro
Zanetta-Barbosa, Darceny
Dechichi, Paula
author_role author
author2 Rezende , Layra Gabriella Pereira de
Reis , Danyella Carolyna Soares dos
Rabelo, Gustavo Davi
Filice, Letícia de Souza Castro
Zanetta-Barbosa, Darceny
Dechichi, Paula
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Flaviana Soares
Rezende , Layra Gabriella Pereira de
Reis , Danyella Carolyna Soares dos
Rabelo, Gustavo Davi
Filice, Letícia de Souza Castro
Zanetta-Barbosa, Darceny
Dechichi, Paula
dc.subject.por.fl_str_mv Ionizing radiation
Hyperbaric oxygenation
Osteogenesis
Collagen.
Radiación ionizante
Oxigenación hiperbárica
Osteogénesis
Colágeno.
Radiação ionizante
Oxigenação hiperbárica
Osteogênese
Colágeno.
topic Ionizing radiation
Hyperbaric oxygenation
Osteogenesis
Collagen.
Radiación ionizante
Oxigenación hiperbárica
Osteogénesis
Colágeno.
Radiação ionizante
Oxigenação hiperbárica
Osteogênese
Colágeno.
description The present study aimed to evaluate the effects of HBO on bone repair after ionizing radiation, by using histomorphometry, computed tomography and polarization microscopy. Twenty male Wistar rats were used. One radiation dose (30 Gy) was administered on the left leg in all animals, and after 30 days, bone defects were created in both femurs. Then, 10 animals received daily HBO sessions (2.5 ATA for 90 minutes), and all animals were euthanized 5 or 7 days after surgery. The femurs were separated into 4 groups (n=5) for each euthanasia time interval: Control (Right femur: Non-irradiated and Non-HBO), RXT (Left femur: Irradiated and Non-HBO), HBO (Right femur: Non-irradiated with HBO), RXT+HBO (Left femur: Irradiated with HBO). The bone cortical and defects were evaluated. Data were analyzed using the Kolmogorov-Smirnov tests, unpaired t-test, and ANOVA with Bonferroni correction. Higher HU values and new bone formation were observed in Control and HBO groups, with improved repair. Group HBO showed predominant Yellow/Orange/Red birefringence of collagen in the defect area. HBO improved bone repair in physiological conditions, with increased blood vessels and bone formation. However, it was not efficient in improving repair and collagen orientation in bone after high doses of ionizing radiation.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/9948
10.33448/rsd-v9i11.9948
url https://rsdjournal.org/index.php/rsd/article/view/9948
identifier_str_mv 10.33448/rsd-v9i11.9948
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/9948/8965
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 11; e4339119948
Research, Society and Development; Vol. 9 Núm. 11; e4339119948
Research, Society and Development; v. 9 n. 11; e4339119948
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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