Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do Centro Universitário La Salle |
Texto Completo: | http://hdl.handle.net/11690/1914 |
Resumo: | Background: Transcranial direct-current stimulation (tDCS) is a noninvasive method of brain stimulation suggested as a therapeutic tool for pain and is related to the reversal of maladaptive plasticity associated with chronic pain. Objectives: This study investigated the effect of tDCS, a non-pharmacological therapy, on local mechanical hyperalgesia, and remote thermal hyperalgesia in rats submitted to orofacial inflammatory pain model, by facial von Frey and hot plate tests, respectively. In addition, we evaluated levels of BDNF, NGF, IL-10 and IL-6 in the brainstem and blood serum of these animals at 24 hours and 7 days after the end of tDCS treatment. Methods: Rats were subjected to temporomandibular joint pain and treated with tDCS. The animals were divided into control, pain and pain + treatment groups. Mechanical and thermal hyperalgesia were evaluated at baseline, 7 days after administration of complete Freund's adjuvant, and immediately, 24 hours, and 7 days after the tDCS treatment. Neuroimmunomodulators levels were determined by ELISA. Statistical analyses were performed by (GEE)/Bonferroni (behavioural tests), three-way ANOVA/SNK (neurochemical tests) and Kruskal-Wallis (histological analysis). Results: Transcranial direct-current stimulation reduced mechanical and thermal hyperalgesia (P < 0.01). We observed interaction between factors (pain and treatment) increasing brainstem BDNF (P < 0.01) and NGF (P < 0.05) levels. Furthermore, we found an increase in IL-6 and IL-10 levels in the brainstem at 24 hours and 7 days after tDCS, respectively. Conclusion: We showed that tDCS reduces thermal and mechanical hyperalgesia induced by orofacial pain until 7 days after treatment. These findings demonstrate that tDCS was effective in the control of orofacial inflammatory pain. |
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Scarabelot, Vanessa L.Oliveira, Carla deMedeiros, Liciane FernandesMacedo, Isabel C. deCioato, Stefania G.Adachi, Lauren Naomi S.Paz, Ana HelenaSouza, Andressa deCaumo, WolneiTorres, Iraci L. S.2021-08-03T14:26:28Z2021-08-03T14:26:28Z2021SCARABELOT, V. L. et al. Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model. Journal of Oral Rehabilitation, v. 46, n. 1, jan., 2021. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/joor.12726. Acesso em: 03 ago. 2021.http://hdl.handle.net/11690/1914Background: Transcranial direct-current stimulation (tDCS) is a noninvasive method of brain stimulation suggested as a therapeutic tool for pain and is related to the reversal of maladaptive plasticity associated with chronic pain. Objectives: This study investigated the effect of tDCS, a non-pharmacological therapy, on local mechanical hyperalgesia, and remote thermal hyperalgesia in rats submitted to orofacial inflammatory pain model, by facial von Frey and hot plate tests, respectively. In addition, we evaluated levels of BDNF, NGF, IL-10 and IL-6 in the brainstem and blood serum of these animals at 24 hours and 7 days after the end of tDCS treatment. Methods: Rats were subjected to temporomandibular joint pain and treated with tDCS. The animals were divided into control, pain and pain + treatment groups. Mechanical and thermal hyperalgesia were evaluated at baseline, 7 days after administration of complete Freund's adjuvant, and immediately, 24 hours, and 7 days after the tDCS treatment. Neuroimmunomodulators levels were determined by ELISA. Statistical analyses were performed by (GEE)/Bonferroni (behavioural tests), three-way ANOVA/SNK (neurochemical tests) and Kruskal-Wallis (histological analysis). Results: Transcranial direct-current stimulation reduced mechanical and thermal hyperalgesia (P < 0.01). We observed interaction between factors (pain and treatment) increasing brainstem BDNF (P < 0.01) and NGF (P < 0.05) levels. Furthermore, we found an increase in IL-6 and IL-10 levels in the brainstem at 24 hours and 7 days after tDCS, respectively. Conclusion: We showed that tDCS reduces thermal and mechanical hyperalgesia induced by orofacial pain until 7 days after treatment. These findings demonstrate that tDCS was effective in the control of orofacial inflammatory pain.Submitted by DSpace Unilasalle (dspace@unilasalle.edu.br) on 2021-08-03T14:26:28Z No. of bitstreams: 1 vlscarabelot.pdf: 1493043 bytes, checksum: c351c068a10bffcb0a6339fc1991fc24 (MD5)Made available in DSpace on 2021-08-03T14:26:28Z (GMT). No. of bitstreams: 1 vlscarabelot.pdf: 1493043 bytes, checksum: c351c068a10bffcb0a6339fc1991fc24 (MD5) Previous issue date: 2021WileyHyperalgesiaNeuromodulationOrofacial inflammatory paintDCSTemporomandibular jointTranscranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional do Centro Universitário La Salleinstname:Universidade La Salle (UNILASALLE)instacron:UNILASALLEORIGINALvlscarabelot.pdfvlscarabelot.pdfOpen Accessapplication/pdf1493043http://svr-net20.unilasalle.edu.br:8080/jspui/bitstream/11690/1914/1/vlscarabelot.pdfc351c068a10bffcb0a6339fc1991fc24MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://svr-net20.unilasalle.edu.br:8080/jspui/bitstream/11690/1914/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5211690/19142021-08-03 11:27:43.036oai:svr-net20.unilasalle.edu.br: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Repositório Institucionalopendoar:2021-08-03T14:27:43Repositório Institucional do Centro Universitário La Salle - Universidade La Salle (UNILASALLE)false |
dc.title.pt_BR.fl_str_mv |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
title |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
spellingShingle |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model Scarabelot, Vanessa L. Hyperalgesia Neuromodulation Orofacial inflammatory pain tDCS Temporomandibular joint |
title_short |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
title_full |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
title_fullStr |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
title_full_unstemmed |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
title_sort |
Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model |
author |
Scarabelot, Vanessa L. |
author_facet |
Scarabelot, Vanessa L. Oliveira, Carla de Medeiros, Liciane Fernandes Macedo, Isabel C. de Cioato, Stefania G. Adachi, Lauren Naomi S. Paz, Ana Helena Souza, Andressa de Caumo, Wolnei Torres, Iraci L. S. |
author_role |
author |
author2 |
Oliveira, Carla de Medeiros, Liciane Fernandes Macedo, Isabel C. de Cioato, Stefania G. Adachi, Lauren Naomi S. Paz, Ana Helena Souza, Andressa de Caumo, Wolnei Torres, Iraci L. S. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Scarabelot, Vanessa L. Oliveira, Carla de Medeiros, Liciane Fernandes Macedo, Isabel C. de Cioato, Stefania G. Adachi, Lauren Naomi S. Paz, Ana Helena Souza, Andressa de Caumo, Wolnei Torres, Iraci L. S. |
dc.subject.por.fl_str_mv |
Hyperalgesia Neuromodulation Orofacial inflammatory pain tDCS Temporomandibular joint |
topic |
Hyperalgesia Neuromodulation Orofacial inflammatory pain tDCS Temporomandibular joint |
description |
Background: Transcranial direct-current stimulation (tDCS) is a noninvasive method of brain stimulation suggested as a therapeutic tool for pain and is related to the reversal of maladaptive plasticity associated with chronic pain. Objectives: This study investigated the effect of tDCS, a non-pharmacological therapy, on local mechanical hyperalgesia, and remote thermal hyperalgesia in rats submitted to orofacial inflammatory pain model, by facial von Frey and hot plate tests, respectively. In addition, we evaluated levels of BDNF, NGF, IL-10 and IL-6 in the brainstem and blood serum of these animals at 24 hours and 7 days after the end of tDCS treatment. Methods: Rats were subjected to temporomandibular joint pain and treated with tDCS. The animals were divided into control, pain and pain + treatment groups. Mechanical and thermal hyperalgesia were evaluated at baseline, 7 days after administration of complete Freund's adjuvant, and immediately, 24 hours, and 7 days after the tDCS treatment. Neuroimmunomodulators levels were determined by ELISA. Statistical analyses were performed by (GEE)/Bonferroni (behavioural tests), three-way ANOVA/SNK (neurochemical tests) and Kruskal-Wallis (histological analysis). Results: Transcranial direct-current stimulation reduced mechanical and thermal hyperalgesia (P < 0.01). We observed interaction between factors (pain and treatment) increasing brainstem BDNF (P < 0.01) and NGF (P < 0.05) levels. Furthermore, we found an increase in IL-6 and IL-10 levels in the brainstem at 24 hours and 7 days after tDCS, respectively. Conclusion: We showed that tDCS reduces thermal and mechanical hyperalgesia induced by orofacial pain until 7 days after treatment. These findings demonstrate that tDCS was effective in the control of orofacial inflammatory pain. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-08-03T14:26:28Z |
dc.date.available.fl_str_mv |
2021-08-03T14:26:28Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SCARABELOT, V. L. et al. Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model. Journal of Oral Rehabilitation, v. 46, n. 1, jan., 2021. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/joor.12726. Acesso em: 03 ago. 2021. |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11690/1914 |
identifier_str_mv |
SCARABELOT, V. L. et al. Transcranial direct-current stimulation reduces nociceptive behaviour in an orofacial pain model. Journal of Oral Rehabilitation, v. 46, n. 1, jan., 2021. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/joor.12726. Acesso em: 03 ago. 2021. |
url |
http://hdl.handle.net/11690/1914 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
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Universidade La Salle (UNILASALLE) |
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UNILASALLE |
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UNILASALLE |
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Repositório Institucional do Centro Universitário La Salle |
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Repositório Institucional do Centro Universitário La Salle |
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