EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Uningá (Online) |
Texto Completo: | https://revista.uninga.br/uninga/article/view/2857 |
Resumo: | Among bacteria with high resistance to antimicrobials, the Gram-negative types, producers of extended-spectrum beta-lactamases (ESBL), have become an extensive problem for public health. ESBL-producing strains are often associated with various infections, such as pneumonia, septicemia, urinary infections, bacteremia, and meningitis. Klebsiella pneumoniae is a Gram-negative bacillus found in the normal microbiota of the human intestine. This microorganism has high rates of ESBL-producing strains that catalyze the hydrolysis of the beta-lactam ring of antimicrobials that have it. The TSDD methodology uses the synergism of the antibiotics diffused in the Muller Hinton agar (MHA) so that the appearance of the phantom area occurs. However, this synergism may present false results in case the distance between the discs containing the drugs is greater than the capacity of antimicrobial diffusion in MHA. ESBL detection is a low-cost technique that has been frequently used in microbiology laboratories because it is easy to perform and provides reliable results, though a consensus as to the standard distances between disks is nonexistent. The objective of this research is to measure how far, considering center to center, this laboratory routine can be used in the double-disc synergism test (TSDD) for phenotypic detection of ESBL. A strain of ESBL-producing K. pneumoniae ATCC ™ 700603 was used to perform this work as recommended by BrCAST. Our results indicate that the variations in distances can bring false-negative results to values greater than 34 mm and smaller than 20 mm. |
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EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniaeAVALIAÇÃO DE DISTÂNCIAS NA DETECÇÃO FENOTÍPICA DE BETALACTAMASE DE ESPECTRO ESTENDIDO EM CEPA PADRÃO DE Klebsiella pneumoniaeAntimicrobianosInfecções por Bactérias Gram-NegativasKlebsiella pneumoniaeMicrobiologiaAntimicrobialsGram-Negative Bacterial InfectionsKlebsiella pneumoniaeMicrobiologyAmong bacteria with high resistance to antimicrobials, the Gram-negative types, producers of extended-spectrum beta-lactamases (ESBL), have become an extensive problem for public health. ESBL-producing strains are often associated with various infections, such as pneumonia, septicemia, urinary infections, bacteremia, and meningitis. Klebsiella pneumoniae is a Gram-negative bacillus found in the normal microbiota of the human intestine. This microorganism has high rates of ESBL-producing strains that catalyze the hydrolysis of the beta-lactam ring of antimicrobials that have it. The TSDD methodology uses the synergism of the antibiotics diffused in the Muller Hinton agar (MHA) so that the appearance of the phantom area occurs. However, this synergism may present false results in case the distance between the discs containing the drugs is greater than the capacity of antimicrobial diffusion in MHA. ESBL detection is a low-cost technique that has been frequently used in microbiology laboratories because it is easy to perform and provides reliable results, though a consensus as to the standard distances between disks is nonexistent. The objective of this research is to measure how far, considering center to center, this laboratory routine can be used in the double-disc synergism test (TSDD) for phenotypic detection of ESBL. A strain of ESBL-producing K. pneumoniae ATCC ™ 700603 was used to perform this work as recommended by BrCAST. Our results indicate that the variations in distances can bring false-negative results to values greater than 34 mm and smaller than 20 mm.Dentre as bactérias com alta resistência a antimicrobianos, as Gram-negativas produtoras de betalactamases de espectro estendido (ESBL) tornaram-se um grande problema de saúde pública. As cepas produtoras de ESBL são frequentemente associadas a pneumonias, septicemias, infecções urinárias, bacteremias e meningites, entre outras infecções. A Klebsiella pneumoniae é um bacilo Gram negativo comum a microbiota normal do intestino humano. Possui altas taxas de cepas produtoras de ESBL que catalisam a hidrólise do anel betalactâmico dos antimicrobianos que o possuem. A metodologia do TSDD utiliza o sinergismo dos antibióticos difundidos no ágar Muller Hinton (MHA), para que ocorra o aparecimento da área fantasma, entretanto o sinergismo pode apresentar resultados falsos quando a distância entre os disco contendo os fármacos é maior do que a capacidade de difusão do antimicrobiano no MHA. A detecção de ESBL é utilizada frequentemente nos laboratórios de microbiologia por ser uma técnica de baixo custo, fácil execução e com resultados confiáveis, entretanto não há um consenso quanto as distâncias padronizadas entre os discos. O objetivo deste trabalho é avaliar até que distâncias de centro a centro podem ser utilizadas na rotina laboratorial na detecção fenotípica de ESBL por teste de sinergismo por disco duplo (TSDD). No presente trabalho foi utilizado uma cepa de ATCCTM 700603 de K. pneumoniae produtora de ESBL, de acordo com o recomentado pelo BrCAST. Nossos resultados apontam que as variações nas distâncias podem trazer resultados falso-negativo para valores maiores do que 34 mm e menores do que 20 mm.Editora Uningá2020-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontextoapplication/pdfhttps://revista.uninga.br/uninga/article/view/285710.46311/2318-0579.57.eUJ2857Revista Uningá; Vol. 57 No. 3 (2020): Revista UNINGÁ; 39-49Revista Uningá; v. 57 n. 3 (2020): Revista UNINGÁ; 39-492318-057910.46311/ru.v57i3reponame:Revista Uningá (Online)instname:Centro Universitário Uningáinstacron:UNINGAporhttps://revista.uninga.br/uninga/article/view/2857/222710.46311/ru.v57i3.2857.g2227Copyright (c) 2020 UNINGÁ JOURNALinfo:eu-repo/semantics/openAccessOliveira, Thais Druscz deAguera, Raul GomesEcker, Alessandra Barrochelli da Silva2020-10-06T22:11:16Zoai:ojs.revista.uninga.br:article/2857Revistahttps://revista.uninga.br/uninga/indexPUBhttps://revista.uninga.br/uninga/oairevistauninga@uninga.edu.br2318-05792318-0579opendoar:2020-10-06T22:11:16Revista Uningá (Online) - Centro Universitário Uningáfalse |
dc.title.none.fl_str_mv |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae AVALIAÇÃO DE DISTÂNCIAS NA DETECÇÃO FENOTÍPICA DE BETALACTAMASE DE ESPECTRO ESTENDIDO EM CEPA PADRÃO DE Klebsiella pneumoniae |
title |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
spellingShingle |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae Oliveira, Thais Druscz de Antimicrobianos Infecções por Bactérias Gram-Negativas Klebsiella pneumoniae Microbiologia Antimicrobials Gram-Negative Bacterial Infections Klebsiella pneumoniae Microbiology |
title_short |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
title_full |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
title_fullStr |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
title_full_unstemmed |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
title_sort |
EVALUATION OF DISTANCES IN THE PHENOTYPIC DETECTION OF BETALACTAMASE OF SPECTRUM EXTENDED IN ATCCTM Klebsiella pneumoniae |
author |
Oliveira, Thais Druscz de |
author_facet |
Oliveira, Thais Druscz de Aguera, Raul Gomes Ecker, Alessandra Barrochelli da Silva |
author_role |
author |
author2 |
Aguera, Raul Gomes Ecker, Alessandra Barrochelli da Silva |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Oliveira, Thais Druscz de Aguera, Raul Gomes Ecker, Alessandra Barrochelli da Silva |
dc.subject.por.fl_str_mv |
Antimicrobianos Infecções por Bactérias Gram-Negativas Klebsiella pneumoniae Microbiologia Antimicrobials Gram-Negative Bacterial Infections Klebsiella pneumoniae Microbiology |
topic |
Antimicrobianos Infecções por Bactérias Gram-Negativas Klebsiella pneumoniae Microbiologia Antimicrobials Gram-Negative Bacterial Infections Klebsiella pneumoniae Microbiology |
description |
Among bacteria with high resistance to antimicrobials, the Gram-negative types, producers of extended-spectrum beta-lactamases (ESBL), have become an extensive problem for public health. ESBL-producing strains are often associated with various infections, such as pneumonia, septicemia, urinary infections, bacteremia, and meningitis. Klebsiella pneumoniae is a Gram-negative bacillus found in the normal microbiota of the human intestine. This microorganism has high rates of ESBL-producing strains that catalyze the hydrolysis of the beta-lactam ring of antimicrobials that have it. The TSDD methodology uses the synergism of the antibiotics diffused in the Muller Hinton agar (MHA) so that the appearance of the phantom area occurs. However, this synergism may present false results in case the distance between the discs containing the drugs is greater than the capacity of antimicrobial diffusion in MHA. ESBL detection is a low-cost technique that has been frequently used in microbiology laboratories because it is easy to perform and provides reliable results, though a consensus as to the standard distances between disks is nonexistent. The objective of this research is to measure how far, considering center to center, this laboratory routine can be used in the double-disc synergism test (TSDD) for phenotypic detection of ESBL. A strain of ESBL-producing K. pneumoniae ATCC ™ 700603 was used to perform this work as recommended by BrCAST. Our results indicate that the variations in distances can bring false-negative results to values greater than 34 mm and smaller than 20 mm. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.uninga.br/uninga/article/view/2857 10.46311/2318-0579.57.eUJ2857 |
url |
https://revista.uninga.br/uninga/article/view/2857 |
identifier_str_mv |
10.46311/2318-0579.57.eUJ2857 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.uninga.br/uninga/article/view/2857/2227 10.46311/ru.v57i3.2857.g2227 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 UNINGÁ JOURNAL info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 UNINGÁ JOURNAL |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora Uningá |
publisher.none.fl_str_mv |
Editora Uningá |
dc.source.none.fl_str_mv |
Revista Uningá; Vol. 57 No. 3 (2020): Revista UNINGÁ; 39-49 Revista Uningá; v. 57 n. 3 (2020): Revista UNINGÁ; 39-49 2318-0579 10.46311/ru.v57i3 reponame:Revista Uningá (Online) instname:Centro Universitário Uningá instacron:UNINGA |
instname_str |
Centro Universitário Uningá |
instacron_str |
UNINGA |
institution |
UNINGA |
reponame_str |
Revista Uningá (Online) |
collection |
Revista Uningá (Online) |
repository.name.fl_str_mv |
Revista Uningá (Online) - Centro Universitário Uningá |
repository.mail.fl_str_mv |
revistauninga@uninga.edu.br |
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1797042165953593344 |