Uso de lipase fúngica na remoção de O&G em efluentes lácteos

• Autor(a) principal: Moura, Jaíne Daiane de
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
• Texto Completo: http://tede.unioeste.br/handle/tede/5223
• topic: Biorremediação; Enzima; Efluente; Bioremediation; Enzyme; Effluent; OUTROS

Oils and greases (O&G) represent a class of pollutants with low affinity for water in the sectors of production of dairy products. Seeking innovative treatment alternatives represents a significant contribution to operational and economic sustainability in the dairy sector. The enzymatic bioremediation, through the use of biocatalysts such as lipases, has shown promising potential. Lipases are versatile catalysts with improved application in biotechnology, since it is a clean technology (white biotechnology), where the reaction conditions are mild, their high enantioselectivity and regioselectivity and easy availability. These enzymes are of particular importance because they specifically hydrolyze oils and greases (O&G), which can be of great interest for the treatment of effluents. In this context, the objective of this work is to use fungal lipase, produced by Solid State Fermentation (FES), in the bioremediation of effluents with a high fat load, resulting from dairy processing. The microorganism used in this work is a strain of filamentous fungus, called JC2017, isolated from the Toledo River, in the City of Toledo-PR. The lipase was produced by FES using sunflower seed bran (Helianthus annuus) as substrate, with a particle size of between 10 and 16 mesh, 55% humidity, 108 spores per gram at 28 ° C. The lipase produced by FES was used in the bioremediation experiments (shaker at 150 rpm, 37 0C and 168 hours) to reduce the O&G of a dairy effluent from Marechal Candido Rondon-PR. In the FES experiments, it is noted that the highest lipolytic activity (210.2 U) was obtained after 96 hours of fermentation. In the bioremediation experiments, a reduction of 91.6% in O&G was obtained in the dairy effluent used. In view of the results, this work showed an efficient treatment where wastewater from the dairy industry can be used for the purpose of reuse in the industrial process and minimize the generation of effluents in the dairy industry treatment plants.