Protease alcalina na alimentação de suínos na fase de crescimento e terminação

Detalhes bibliográficos
Autor(a) principal: Arndt, Stefani Natani dos Santos
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: https://tede.unioeste.br/handle/tede/6626
Resumo: The dependence on ingredients such as soybean meal in swine feed influences directly the cost of production, intensifying studies involving the use of feed additives as the protease enzyme. The objective of this study was to evaluate the addition of alkaline protease in diets, with and without reduction of crude protein (PB), for growing-finishing pigs, on zootechnical performance, apparent digestibility of nutrients, blood parameters and carcass and meat attributes. A total of 40 male pigs with initial body weight of 26.28 ± 1.24 kg were allocated in a completely randomized experimental design, consisting of five treatments: (1) CP: positive control, without PB reduction and without enzyme, and (2) CP + 0.30: CP with the addition of 0.30 kg of enzyme/t of feed (3) CN: negative control, with a reduction of 2 and 1% of the PB content in the growth and finishing phases, respectively, without enzyme, (4) CN + 0.15: CN with addition of 0.15 kg of enzyme/t of feed, (5) CN + 0.30: CN with addition of 0.30 kg of enzyme/t of feed, with eight repetitions, with the animal as the experimental unit. The results indicated that there was a treatment effect on average daily feed intake (CRDM), daily body weight gain (GPCD) and feed efficiency (EA) for pigs in growth phase II (P<0.0001, P= 0.0398 and P=0.0007). Through the analysis of orthogonal contrasts, it was observed that the CN animals had (P≤0.05) greater EA compared to those that consumed CN+0.15 and CN+0.30, although the worst GPCD and lowest CRDM was verified for the animals that received CN+0.30. There was no effect (P>0.05) of treatment on the zootechnical performance variables in the finishing phase, although an inclination (P=0.0729) was observed on the GPCD. There was an effect (P≤0.05) on apparent digestibility coefficient of dry matter (CDAMS), digestible dry matter (MSD), apparent digestibility coefficient of organic matter (CDAMO), digestible organic matter (MOD) and available protein (PD) in growing phase II. Comparisons among the average of treatments tested by orthogonal contrasts indicated that animals from the CN treatment showed a reduction in CDA when compared to CN+0.15 and CN+0.30. In finishing phase II, there was a difference (P≤0.05) on MSD, CDAMO, MOD, PD and digestibility coefficient apparent of gross energy (CDAEB). The results indicated that CN animals had lower CDA when compared to those that received CN+0.15 and CN+0.30, except for PD. There was no effect (P>0.05) of treatments on blood parameters in growth phase II. However, in the finishing phase II, the pigs in the CP group showed (P≤0.05) an increase of 27.65% in albumin concentration compared to those with CP+0.30. Also, animals in the CP group had (P≤0.05) an increase in PT and globulin concentrations compared to those in the CN group. The pigs that consumed the treatment based on CN and CP+0.30 showed (P≤0.05) an increase in the luminosity of the longissimus thoracis muscle compared to the CP, as well as it was verified (P=0.015) greater luminosity in the piglet muscle from the CN group compared to the CP group. In conclusion, the reduction of PB in the diet by 2% and 1%, growth and finishing respectively, maintaining the amino acid supplementation up to valine, does not modify performance, blood characteristics, carcass and meat characteristics. The addition of the protease enzyme improves digestibility.
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spelling Carvalho, Paulo Levi de Oliveirahttp://lattes.cnpq.br/6095801852303147Genova, Jansller Luizhttp://lattes.cnpq.br/6161598140181205Carvalho, Paulo Levi de Oliveirahttp://lattes.cnpq.br/6095801852303147Genova, Jansller Luizhttp://lattes.cnpq.br/6161598140181205Nunes, Ricardo Viannahttp://lattes.cnpq.br/1731194927960322Saraiva, Alyssonhttp://lattes.cnpq.br/5011340575974094http://lattes.cnpq.br/7328883384927222Arndt, Stefani Natani dos Santos2023-05-09T14:32:45Z2022-12-19ARNDT, Stefani Natani dos Santos. Protease alcalina na alimentação de suínos na fase de crescimento e terminação. 2022. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2022.https://tede.unioeste.br/handle/tede/6626The dependence on ingredients such as soybean meal in swine feed influences directly the cost of production, intensifying studies involving the use of feed additives as the protease enzyme. The objective of this study was to evaluate the addition of alkaline protease in diets, with and without reduction of crude protein (PB), for growing-finishing pigs, on zootechnical performance, apparent digestibility of nutrients, blood parameters and carcass and meat attributes. A total of 40 male pigs with initial body weight of 26.28 ± 1.24 kg were allocated in a completely randomized experimental design, consisting of five treatments: (1) CP: positive control, without PB reduction and without enzyme, and (2) CP + 0.30: CP with the addition of 0.30 kg of enzyme/t of feed (3) CN: negative control, with a reduction of 2 and 1% of the PB content in the growth and finishing phases, respectively, without enzyme, (4) CN + 0.15: CN with addition of 0.15 kg of enzyme/t of feed, (5) CN + 0.30: CN with addition of 0.30 kg of enzyme/t of feed, with eight repetitions, with the animal as the experimental unit. The results indicated that there was a treatment effect on average daily feed intake (CRDM), daily body weight gain (GPCD) and feed efficiency (EA) for pigs in growth phase II (P<0.0001, P= 0.0398 and P=0.0007). Through the analysis of orthogonal contrasts, it was observed that the CN animals had (P≤0.05) greater EA compared to those that consumed CN+0.15 and CN+0.30, although the worst GPCD and lowest CRDM was verified for the animals that received CN+0.30. There was no effect (P>0.05) of treatment on the zootechnical performance variables in the finishing phase, although an inclination (P=0.0729) was observed on the GPCD. There was an effect (P≤0.05) on apparent digestibility coefficient of dry matter (CDAMS), digestible dry matter (MSD), apparent digestibility coefficient of organic matter (CDAMO), digestible organic matter (MOD) and available protein (PD) in growing phase II. Comparisons among the average of treatments tested by orthogonal contrasts indicated that animals from the CN treatment showed a reduction in CDA when compared to CN+0.15 and CN+0.30. In finishing phase II, there was a difference (P≤0.05) on MSD, CDAMO, MOD, PD and digestibility coefficient apparent of gross energy (CDAEB). The results indicated that CN animals had lower CDA when compared to those that received CN+0.15 and CN+0.30, except for PD. There was no effect (P>0.05) of treatments on blood parameters in growth phase II. However, in the finishing phase II, the pigs in the CP group showed (P≤0.05) an increase of 27.65% in albumin concentration compared to those with CP+0.30. Also, animals in the CP group had (P≤0.05) an increase in PT and globulin concentrations compared to those in the CN group. The pigs that consumed the treatment based on CN and CP+0.30 showed (P≤0.05) an increase in the luminosity of the longissimus thoracis muscle compared to the CP, as well as it was verified (P=0.015) greater luminosity in the piglet muscle from the CN group compared to the CP group. In conclusion, the reduction of PB in the diet by 2% and 1%, growth and finishing respectively, maintaining the amino acid supplementation up to valine, does not modify performance, blood characteristics, carcass and meat characteristics. The addition of the protease enzyme improves digestibility.A dependência de ingredientes como o farelo de soja na alimentação de suínos influencia diretamente no custo de produção, intensificando estudos envolvendo o uso de aditivos alimentares como a enzima protease. O objetivo deste estudo foi avaliar a adição de protease alcalina em dietas, com e sem redução de proteína bruta (PB), para suínos em crescimentoterminação, sobre o desempenho zootécnico, digestibilidade aparente dos nutrientes, parâmetros sanguíneos e atributos de carcaça e carne. Um total de 40 suínos machos inteiros com peso corporal inicial de 26,28 ± 1,24 kg foram alocados em um delineamento experimental inteiramente casualizados, composto de cinco tratamentos: (1) CP: controle positivo, sem redução de PB e sem enzima, e (2) CP + 0,30: CP com adição de 0,30 kg de enzima/t de ração (3) CN: controle negativo, com redução de 2 e 1% do conteúdo de PB nas fases de crescimento e terminação, respectivamente, sem enzima, (4) CN + 0,15: CN com adição de 0,15 kg de enzima/t de ração, (5) CN + 0,30: CN com adição de 0,30 kg de enzima/t de ração, com oito repetições, sendo o animal a unidade experimental. Os resultados indicaram que foram verificados efeito de tratamento sobre o consumo médio de ração diário (CRDM), ganho de peso corporal diário (GPCD) e eficiência alimentar (EA) para suínos na fase de crescimento II (P<0,0001, P=0,0398 e P=0,0007). Por meio da análise de contrastes ortogonais, foi observado que os animais do CN tiveram (P≤0,05) maior EA em comparação aos que consumiram CN+0,15 e CN+0,30, embora o pior GPCD e menor CRDM foi verificado para os animais que receberam CN+0,30. Não foi verificado efeito (P>0,05) de tratamento sobre as variáveis de desempenho zootécnico na fase de terminação, embora uma tendência (P=0,0729) foi observada sobre o GPCD. Houve efeito (P≤0,05) sobre coeficiente de digestibilidade aparente da matéria seca (CDAMS), matéria seca digestível (MSD), coeficiente de digestibilidade aparente da matéria orgânica (CDAMO), matéria orgânica digestível (MOD) e proteína disponível (PD) na fase de crescimento II. As comparações entre médias de tratamentos testadas por contrastes ortogonais indicaram que animais do tratamento CN apresentaram redução nos CDA quando comparados aos CN+0,15 e CN+0,30. Na fase de terminação II, houve diferença (P≤0,05) sobre MSD, CDAMO, MOD, PD e coeficiente de digestibilidade aparente da energia bruta (CDAEB). Os resultados indicaram que animais do CN tiveram menores CDA quando comparados aos que receberam CN+0,15 e CN+0,30, exceto sobre a PD. Não houve efeito (P>0,05) de tratamentos sobre os parâmetros sanguíneos na fase de crescimento II. Entretanto, na fase de terminação II, os suínos do grupo CP apresentaram (P≤0,05) um acréscimo de 27,65% na concentração de albumina em comparação aos com CP+0,30. Também, os animais do grupo CP tiveram (P≤0,05) aumento nas concentrações de PT e globulina em relação àqueles do grupo CN. Os suínos que consumiram o tratamento à base de CN e CP+0,30 apresentaram (P≤0,05) um aumento na luminosidade do músculo longissimus thoracis comparados aos CP, bem como foi verificada (P=0,015) maior luminosidade no músculo de suínos do grupo CN em comparação ao grupo CP. Como conclusão, a redução de PB na dieta em 2% e 1%, crescimento e terminação respectivamente, mantendo-se a suplementação de aminoácidos até a valina, não altera desempenho, características sanguíneas e características de carcaça e carne. A adição da enzima protease melhora a digestibilidade.Submitted by Helena Bejio (helena.bejio@unioeste.br) on 2023-05-09T14:32:45Z No. of bitstreams: 2 Stefani_Arndt_2022.pdf: 1706183 bytes, checksum: fd0524ff7b3cbc9011a652194b204c53 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2023-05-09T14:32:45Z (GMT). No. of bitstreams: 2 Stefani_Arndt_2022.pdf: 1706183 bytes, checksum: fd0524ff7b3cbc9011a652194b204c53 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2022-12-19Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqapplication/pdfpor-6392337873870130111500Universidade Estadual do Oeste do ParanáMarechal Cândido RondonPrograma de Pós-Graduação em ZootecniaUNIOESTEBrasilCentro de Ciências Agráriashttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtributos de carneDesempenhoDigestibilidadeEnzimaNutrição de suínosParâmetros sanguíneosCIÊNCIAS AGRÁRIAS:ZOOTECNIAProtease alcalina na alimentação de suínos na fase de crescimento e terminaçãoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-3881065194686295060600600600-7585593950289668980-2555911436985713659reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALStefani_Arndt_2022.pdfStefani_Arndt_2022.pdfapplication/pdf1706183http://tede.unioeste.br:8080/tede/bitstream/tede/6626/5/Stefani_Arndt_2022.pdffd0524ff7b3cbc9011a652194b204c53MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv Protease alcalina na alimentação de suínos na fase de crescimento e terminação
title Protease alcalina na alimentação de suínos na fase de crescimento e terminação
spellingShingle Protease alcalina na alimentação de suínos na fase de crescimento e terminação
Arndt, Stefani Natani dos Santos
Atributos de carne
Desempenho
Digestibilidade
Enzima
Nutrição de suínos
Parâmetros sanguíneos
CIÊNCIAS AGRÁRIAS:ZOOTECNIA
title_short Protease alcalina na alimentação de suínos na fase de crescimento e terminação
title_full Protease alcalina na alimentação de suínos na fase de crescimento e terminação
title_fullStr Protease alcalina na alimentação de suínos na fase de crescimento e terminação
title_full_unstemmed Protease alcalina na alimentação de suínos na fase de crescimento e terminação
title_sort Protease alcalina na alimentação de suínos na fase de crescimento e terminação
author Arndt, Stefani Natani dos Santos
author_facet Arndt, Stefani Natani dos Santos
author_role author
dc.contributor.advisor1.fl_str_mv Carvalho, Paulo Levi de Oliveira
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6095801852303147
dc.contributor.advisor-co1.fl_str_mv Genova, Jansller Luiz
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/6161598140181205
dc.contributor.referee1.fl_str_mv Carvalho, Paulo Levi de Oliveira
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6095801852303147
dc.contributor.referee2.fl_str_mv Genova, Jansller Luiz
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/6161598140181205
dc.contributor.referee3.fl_str_mv Nunes, Ricardo Vianna
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1731194927960322
dc.contributor.referee4.fl_str_mv Saraiva, Alysson
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/5011340575974094
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7328883384927222
dc.contributor.author.fl_str_mv Arndt, Stefani Natani dos Santos
contributor_str_mv Carvalho, Paulo Levi de Oliveira
Genova, Jansller Luiz
Carvalho, Paulo Levi de Oliveira
Genova, Jansller Luiz
Nunes, Ricardo Vianna
Saraiva, Alysson
dc.subject.por.fl_str_mv Atributos de carne
Desempenho
Digestibilidade
Enzima
Nutrição de suínos
Parâmetros sanguíneos
topic Atributos de carne
Desempenho
Digestibilidade
Enzima
Nutrição de suínos
Parâmetros sanguíneos
CIÊNCIAS AGRÁRIAS:ZOOTECNIA
dc.subject.cnpq.fl_str_mv CIÊNCIAS AGRÁRIAS:ZOOTECNIA
description The dependence on ingredients such as soybean meal in swine feed influences directly the cost of production, intensifying studies involving the use of feed additives as the protease enzyme. The objective of this study was to evaluate the addition of alkaline protease in diets, with and without reduction of crude protein (PB), for growing-finishing pigs, on zootechnical performance, apparent digestibility of nutrients, blood parameters and carcass and meat attributes. A total of 40 male pigs with initial body weight of 26.28 ± 1.24 kg were allocated in a completely randomized experimental design, consisting of five treatments: (1) CP: positive control, without PB reduction and without enzyme, and (2) CP + 0.30: CP with the addition of 0.30 kg of enzyme/t of feed (3) CN: negative control, with a reduction of 2 and 1% of the PB content in the growth and finishing phases, respectively, without enzyme, (4) CN + 0.15: CN with addition of 0.15 kg of enzyme/t of feed, (5) CN + 0.30: CN with addition of 0.30 kg of enzyme/t of feed, with eight repetitions, with the animal as the experimental unit. The results indicated that there was a treatment effect on average daily feed intake (CRDM), daily body weight gain (GPCD) and feed efficiency (EA) for pigs in growth phase II (P<0.0001, P= 0.0398 and P=0.0007). Through the analysis of orthogonal contrasts, it was observed that the CN animals had (P≤0.05) greater EA compared to those that consumed CN+0.15 and CN+0.30, although the worst GPCD and lowest CRDM was verified for the animals that received CN+0.30. There was no effect (P>0.05) of treatment on the zootechnical performance variables in the finishing phase, although an inclination (P=0.0729) was observed on the GPCD. There was an effect (P≤0.05) on apparent digestibility coefficient of dry matter (CDAMS), digestible dry matter (MSD), apparent digestibility coefficient of organic matter (CDAMO), digestible organic matter (MOD) and available protein (PD) in growing phase II. Comparisons among the average of treatments tested by orthogonal contrasts indicated that animals from the CN treatment showed a reduction in CDA when compared to CN+0.15 and CN+0.30. In finishing phase II, there was a difference (P≤0.05) on MSD, CDAMO, MOD, PD and digestibility coefficient apparent of gross energy (CDAEB). The results indicated that CN animals had lower CDA when compared to those that received CN+0.15 and CN+0.30, except for PD. There was no effect (P>0.05) of treatments on blood parameters in growth phase II. However, in the finishing phase II, the pigs in the CP group showed (P≤0.05) an increase of 27.65% in albumin concentration compared to those with CP+0.30. Also, animals in the CP group had (P≤0.05) an increase in PT and globulin concentrations compared to those in the CN group. The pigs that consumed the treatment based on CN and CP+0.30 showed (P≤0.05) an increase in the luminosity of the longissimus thoracis muscle compared to the CP, as well as it was verified (P=0.015) greater luminosity in the piglet muscle from the CN group compared to the CP group. In conclusion, the reduction of PB in the diet by 2% and 1%, growth and finishing respectively, maintaining the amino acid supplementation up to valine, does not modify performance, blood characteristics, carcass and meat characteristics. The addition of the protease enzyme improves digestibility.
publishDate 2022
dc.date.issued.fl_str_mv 2022-12-19
dc.date.accessioned.fl_str_mv 2023-05-09T14:32:45Z
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dc.identifier.citation.fl_str_mv ARNDT, Stefani Natani dos Santos. Protease alcalina na alimentação de suínos na fase de crescimento e terminação. 2022. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2022.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6626
identifier_str_mv ARNDT, Stefani Natani dos Santos. Protease alcalina na alimentação de suínos na fase de crescimento e terminação. 2022. 52 f. Dissertação (Mestrado em Zootecnia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2022.
url https://tede.unioeste.br/handle/tede/6626
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language por
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Zootecnia
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Ciências Agrárias
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Marechal Cândido Rondon
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