Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Texto Completo: | http://tede.unioeste.br/handle/tede/4305 |
Resumo: | The hemerocale is a monoic plant used in inter and intraspecific hybridizations, which has given rise to a great diversity of cultivars with different colors and forms through programs of improvement. Taking the aforementioned into account, the objective of this work was to establish a culture medium to evaluate pollen viability in day-old cultivars and to evaluate the dehydration and storage conditions of pollen grains regarding their germination and viability. A sequential experiment was carried out in the laboratory, where the cultivar Morgana AS Piske was used for the establishment of the culture medium, in which the pH ranges (4, 5, 6, 7) together with concentrations of agar (4, 6, 8, 10 g L-1). In the following test, concentrations of sucrose (0, 30, 60, 90, 120 g L-1) were tested. Afterwards, concentrations of boric acid (0, 400, 800, 1200 mg L-1) were added to the medium and, finally, calcium nitrate concentrations (0, 200, 400 and 800 mg L-1). After the establishment of the culture medium, the incubation time required for maximum germination of the pollen grains was evaluated. The obtained medium was tested in four cultivars, aiming at the verification of germination percentages. For the second part of the experiments, four times (0, 12, 24, 48 hours) and two drying sites (BOD greenhouse at 25ºC and silica gel desiccator) were evaluated. In vitro germination and pollen viability tests were performed. After the determination of the best time and place of drying, the pollen grains were stored. They were stored in a freezer at -20 ± 3ºC, refrigerator at 4 ± 3ºC and BOD 25ºC, for 0, 7, 14, 21 and 28 days. After storage, germination and viability tests were repeated. The highest percentage of in vitro germination occurred with the concentrations of 4 g L-1 agar, 74.6 g L-1 sucrose, 800 mg L-1 boric acid, 590 mg L-1 calcium nitrate, pH of 5.74 and ideal incubation time of 3 hours, being cv. Regina the one with the highest percentage of germination. The pollen grains of the hemerocale cultivars had a higher percentage of germination when fresh, and there was no need for dehydration. The best storage site was the freezer, where the pollen kept 50% of its germination, even after 28 days of storage. The 1% dye 2,3,5-triphenyltetrazolium (TTC) was not efficient in determining the pollen viability of hemerocale pollen. |
id |
UNIOESTE-1_5e4bf388597cf4873d81d3554c727adc |
---|---|
oai_identifier_str |
oai:tede.unioeste.br:tede/4305 |
network_acronym_str |
UNIOESTE-1 |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
repository_id_str |
|
spelling |
Villa, Fabíolahttp://lattes.cnpq.br/1043592167112136Fogaça, Luciana Alveshttp://lattes.cnpq.br/1052718598551568Villa, Fabíolahttp://lattes.cnpq.br/1043592167112136Echer, Márcia de Moraeshttp://lattes.cnpq.br/3526849044127052Silva, Daniel Fernandes dahttp://lattes.cnpq.br/6562731599312560Yamamoto, Lilian Yukarihttp://lattes.cnpq.br/4913331884134387http://lattes.cnpq.br/2965403722393870Eberling, Tatiane2019-05-28T00:48:04Z2019-02-27EBERLING, Tatiane. Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale. 2019. 27 f. Dissertação ( Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019.http://tede.unioeste.br/handle/tede/4305The hemerocale is a monoic plant used in inter and intraspecific hybridizations, which has given rise to a great diversity of cultivars with different colors and forms through programs of improvement. Taking the aforementioned into account, the objective of this work was to establish a culture medium to evaluate pollen viability in day-old cultivars and to evaluate the dehydration and storage conditions of pollen grains regarding their germination and viability. A sequential experiment was carried out in the laboratory, where the cultivar Morgana AS Piske was used for the establishment of the culture medium, in which the pH ranges (4, 5, 6, 7) together with concentrations of agar (4, 6, 8, 10 g L-1). In the following test, concentrations of sucrose (0, 30, 60, 90, 120 g L-1) were tested. Afterwards, concentrations of boric acid (0, 400, 800, 1200 mg L-1) were added to the medium and, finally, calcium nitrate concentrations (0, 200, 400 and 800 mg L-1). After the establishment of the culture medium, the incubation time required for maximum germination of the pollen grains was evaluated. The obtained medium was tested in four cultivars, aiming at the verification of germination percentages. For the second part of the experiments, four times (0, 12, 24, 48 hours) and two drying sites (BOD greenhouse at 25ºC and silica gel desiccator) were evaluated. In vitro germination and pollen viability tests were performed. After the determination of the best time and place of drying, the pollen grains were stored. They were stored in a freezer at -20 ± 3ºC, refrigerator at 4 ± 3ºC and BOD 25ºC, for 0, 7, 14, 21 and 28 days. After storage, germination and viability tests were repeated. The highest percentage of in vitro germination occurred with the concentrations of 4 g L-1 agar, 74.6 g L-1 sucrose, 800 mg L-1 boric acid, 590 mg L-1 calcium nitrate, pH of 5.74 and ideal incubation time of 3 hours, being cv. Regina the one with the highest percentage of germination. The pollen grains of the hemerocale cultivars had a higher percentage of germination when fresh, and there was no need for dehydration. The best storage site was the freezer, where the pollen kept 50% of its germination, even after 28 days of storage. The 1% dye 2,3,5-triphenyltetrazolium (TTC) was not efficient in determining the pollen viability of hemerocale pollen.A hemerocale é uma planta monóica usada em hibridações inter e intraespecíficas, o que tem proporcionado o surgimento de uma grande diversidade de cultivares com diferentes cores e formas por meio de programas de melhoramento. Diante do exposto, objetivou-se com o presente trabalho estabelecer um meio de cultura para avaliação da viabilidade polínica em cultivares de hemerocale e avaliar condições de desidratação e armazenamento de grãos de pólen sobre sua germinação e viabilidade. Foram montados experimentos sequenciais em laboratório, onde, no primeiro, a cultivar Morgana A. S. Piske, foi utilizada para a realização dos experimentos de estabelecimento do meio de cultura, nos quais testou-se faixas de pH (4, 5, 6, 7), juntamente a concentrações de ágar (4, 6, 8, 10 g L-1). No teste seguinte, testou-se concentrações de sacarose (0, 30, 60, 90, 120 g L-1). Prosseguindo, adicionou-se ao meio concentrações de ácido bórico (0, 400, 800, 1200 mg L-1) e, por fim, foram concentrações de nitrato de cálcio (0, 200, 400 e 800 mg L-1). Após o estabelecimento do meio de cultura, avaliou-se o tempo de incubação necessário para máxima germinação dos grãos de pólen. O meio obtido foi testado em quatro cultivares, visando a verificação das porcentagens de germinação. Para a segunda parte dos experimentos, avaliaram-se os tempos (0, 12, 24, 48 horas) e locais de secagem (estufa tipo BOD a 25ºC e dessecador de sílica gel). Foram realizados testes de germinação in vitro e viabilidade polínica. Após a determinação do melhor tempo e local de secagem, realizou-se o armazenamento dos grãos de pólen; os mesmos foram armazenados em freezer a -20 ± 3ºC, geladeira a 4 ± 3ºC e estufa BOD 25ºC, por 0, 7, 14, 21 e 28 dias. Após o armazenamento, repetiram-se os testes de germinação e viabilidade. O maior percentual de germinação in vitro ocorreu com as concentrações de 4 g L-1 de ágar, 74,6 g L-1 de sacarose, 800 mg L-1 de ácido bórico, 590 mg L-1 de nitrato de cálcio, com pH de 5,74 e tempo ideal de incubação de 3 horas, sendo a cv. Regina aquela com maior percentual de germinação. Os grãos de pólen das cultivares de hemerocale apresentaram maior porcentagem de germinação quando frescos, não havendo a necessidade de desidratação. O melhor local de armazenagem foi o freezer, onde o pólen manteve 50% de sua germinação, mesmo após 28 dias de armazenamento. O corante 2,3,5 trifeniltetrazólio (TTC) a 1% não foi eficiente na determinação da viabilidade polínica do pólen de hemerocale.Submitted by Helena Bejio (helena.bejio@unioeste.br) on 2019-05-28T00:48:04Z No. of bitstreams: 2 Tatiane_Eberling_2019.pdf: 618159 bytes, checksum: 2823baa45170126d8f7d70beecf69448 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2019-05-28T00:48:04Z (GMT). No. of bitstreams: 2 Tatiane_Eberling_2019.pdf: 618159 bytes, checksum: 2823baa45170126d8f7d70beecf69448 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2019-02-27Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfpor-6392337873870130111500Universidade Estadual do Oeste do ParanáMarechal Cândido RondonPrograma de Pós-Graduação em AgronomiaUNIOESTEBrasilCentro de Ciências Agráriashttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessHemerocallis x hybrida Hort.PalinologiaGerminação in vitroConservação in vitroCIÊNCIAS AGRÁRIAS:AGRONOMIAEstabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocaleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis5624066117035054290600600600-75855939502896689802075167498588264571reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALTatiane_Eberling_2019.pdfTatiane_Eberling_2019.pdfapplication/pdf618159http://tede.unioeste.br:8080/tede/bitstream/tede/4305/5/Tatiane_Eberling_2019.pdf2823baa45170126d8f7d70beecf69448MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-843http://tede.unioeste.br:8080/tede/bitstream/tede/4305/2/license_url321f3992dd3875151d8801b773ab32edMD52license_textlicense_texttext/html; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/4305/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/4305/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/4305/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/43052019-05-27 21:52:28.459oai:tede.unioeste.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2019-05-28T00:52:28Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false |
dc.title.por.fl_str_mv |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
title |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
spellingShingle |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale Eberling, Tatiane Hemerocallis x hybrida Hort. Palinologia Germinação in vitro Conservação in vitro CIÊNCIAS AGRÁRIAS:AGRONOMIA |
title_short |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
title_full |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
title_fullStr |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
title_full_unstemmed |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
title_sort |
Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale |
author |
Eberling, Tatiane |
author_facet |
Eberling, Tatiane |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Villa, Fabíola |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1043592167112136 |
dc.contributor.advisor-co1.fl_str_mv |
Fogaça, Luciana Alves |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1052718598551568 |
dc.contributor.referee1.fl_str_mv |
Villa, Fabíola |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1043592167112136 |
dc.contributor.referee2.fl_str_mv |
Echer, Márcia de Moraes |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3526849044127052 |
dc.contributor.referee3.fl_str_mv |
Silva, Daniel Fernandes da |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/6562731599312560 |
dc.contributor.referee4.fl_str_mv |
Yamamoto, Lilian Yukari |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/4913331884134387 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2965403722393870 |
dc.contributor.author.fl_str_mv |
Eberling, Tatiane |
contributor_str_mv |
Villa, Fabíola Fogaça, Luciana Alves Villa, Fabíola Echer, Márcia de Moraes Silva, Daniel Fernandes da Yamamoto, Lilian Yukari |
dc.subject.por.fl_str_mv |
Hemerocallis x hybrida Hort. Palinologia Germinação in vitro Conservação in vitro |
topic |
Hemerocallis x hybrida Hort. Palinologia Germinação in vitro Conservação in vitro CIÊNCIAS AGRÁRIAS:AGRONOMIA |
dc.subject.cnpq.fl_str_mv |
CIÊNCIAS AGRÁRIAS:AGRONOMIA |
description |
The hemerocale is a monoic plant used in inter and intraspecific hybridizations, which has given rise to a great diversity of cultivars with different colors and forms through programs of improvement. Taking the aforementioned into account, the objective of this work was to establish a culture medium to evaluate pollen viability in day-old cultivars and to evaluate the dehydration and storage conditions of pollen grains regarding their germination and viability. A sequential experiment was carried out in the laboratory, where the cultivar Morgana AS Piske was used for the establishment of the culture medium, in which the pH ranges (4, 5, 6, 7) together with concentrations of agar (4, 6, 8, 10 g L-1). In the following test, concentrations of sucrose (0, 30, 60, 90, 120 g L-1) were tested. Afterwards, concentrations of boric acid (0, 400, 800, 1200 mg L-1) were added to the medium and, finally, calcium nitrate concentrations (0, 200, 400 and 800 mg L-1). After the establishment of the culture medium, the incubation time required for maximum germination of the pollen grains was evaluated. The obtained medium was tested in four cultivars, aiming at the verification of germination percentages. For the second part of the experiments, four times (0, 12, 24, 48 hours) and two drying sites (BOD greenhouse at 25ºC and silica gel desiccator) were evaluated. In vitro germination and pollen viability tests were performed. After the determination of the best time and place of drying, the pollen grains were stored. They were stored in a freezer at -20 ± 3ºC, refrigerator at 4 ± 3ºC and BOD 25ºC, for 0, 7, 14, 21 and 28 days. After storage, germination and viability tests were repeated. The highest percentage of in vitro germination occurred with the concentrations of 4 g L-1 agar, 74.6 g L-1 sucrose, 800 mg L-1 boric acid, 590 mg L-1 calcium nitrate, pH of 5.74 and ideal incubation time of 3 hours, being cv. Regina the one with the highest percentage of germination. The pollen grains of the hemerocale cultivars had a higher percentage of germination when fresh, and there was no need for dehydration. The best storage site was the freezer, where the pollen kept 50% of its germination, even after 28 days of storage. The 1% dye 2,3,5-triphenyltetrazolium (TTC) was not efficient in determining the pollen viability of hemerocale pollen. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-05-28T00:48:04Z |
dc.date.issued.fl_str_mv |
2019-02-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
EBERLING, Tatiane. Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale. 2019. 27 f. Dissertação ( Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br/handle/tede/4305 |
identifier_str_mv |
EBERLING, Tatiane. Estabelecimento do meio de cultura e conservação da viabilidade polínica de hemerocale. 2019. 27 f. Dissertação ( Mestrado em Agronomia) - Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon, 2019. |
url |
http://tede.unioeste.br/handle/tede/4305 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.program.fl_str_mv |
5624066117035054290 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
dc.relation.department.fl_str_mv |
-7585593950289668980 |
dc.relation.sponsorship.fl_str_mv |
2075167498588264571 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Marechal Cândido Rondon |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Centro de Ciências Agrárias |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Marechal Cândido Rondon |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE instname:Universidade Estadual do Oeste do Paraná (UNIOESTE) instacron:UNIOESTE |
instname_str |
Universidade Estadual do Oeste do Paraná (UNIOESTE) |
instacron_str |
UNIOESTE |
institution |
UNIOESTE |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
collection |
Biblioteca Digital de Teses e Dissertações do UNIOESTE |
bitstream.url.fl_str_mv |
http://tede.unioeste.br:8080/tede/bitstream/tede/4305/5/Tatiane_Eberling_2019.pdf http://tede.unioeste.br:8080/tede/bitstream/tede/4305/2/license_url http://tede.unioeste.br:8080/tede/bitstream/tede/4305/3/license_text http://tede.unioeste.br:8080/tede/bitstream/tede/4305/4/license_rdf http://tede.unioeste.br:8080/tede/bitstream/tede/4305/1/license.txt |
bitstream.checksum.fl_str_mv |
2823baa45170126d8f7d70beecf69448 321f3992dd3875151d8801b773ab32ed d41d8cd98f00b204e9800998ecf8427e d41d8cd98f00b204e9800998ecf8427e bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE) |
repository.mail.fl_str_mv |
biblioteca.repositorio@unioeste.br |
_version_ |
1801124559358787584 |