Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Carolina Pereira da
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br/handle/tede/4896
Resumo: Chicken is an animal protein most consumed in Brazil, which is a leader in the world ranking of exports of this food. Its high consumption must be low price and high nutritional value, however, it is a vehicle for pathogenic microorganisms such as Salmonella spp. Antimicrobial agents have been used in agriculture for many years to control the proliferation of these microorganisms and this favored prolonged stay or surgery of resistance mechanisms in strains of Salmonella spp. Therefore, the aim of the present study was to isolate and characterize Salmonella spp. from cuts of chickens marketed in the city of Francisco Beltrão - Paraná and verify the front resistance profile for antimicrobials used in human medicine. 40 samples of chicken cuts were purchased from supermarkets in Francisco Beltrão - PR. The microbiological analyzes followed the stages of preenrichment, selective enrichment and differential plating. The suspected colonies were subjected to biochemical tests carried out in a Kit for the identification of enterobacteria (NEWPROV). Four colonies from each sample were selected with Salmonella spp. biochemical behavior, totaling 28 isolates, to perform the antimicrobial sensitivity test. Samples that showed resistance to ceftriaxone were subjected to extended spectrum beta lactamases. All isolates subjected to antimicrobial susceptibility testing demonstrated sensitivity to meropenem, whereas 100% of the isolates were resistant to nalidixic acid, a high resistance to beta lactam antimicrobials was also observed. Seventeen different resistance profiles were found, with 42.85% showing a multi-resistance profile and 21.42% of the isolates showing a phenotype for ESBL production. The frequency of batches contaminated with Salmonella spp. of 17.5% in chicken cuts destined for commercialization, points to the importance of proper cooking and handling of these foods for the prevention of salmonellosis outbreaks. The high resistance to antimicrobials and their potential for dissemination found in this study is a worrying fact, as it puts at risk the treatment of infections caused by Salmonella spp.
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spelling Casaril, Kérley Braga Pereira Bentohttp://lattes.cnpq.br/6429832392023465Casaril, Kérley Braga Pereira Bentohttp://lattes.cnpq.br/6429832392023465Lucio, Léia Carolinahttp://lattes.cnpq.br/9357029990194108Benedetti, Volmir Pitthttp://lattes.cnpq.br/8681216826241489http://lattes.cnpq.br/9205567887145256Silva, Ana Carolina Pereira da2020-09-01T17:09:41Z2020-03-03SILVA, Ana Carolina Pereira da. Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos. 2020. 71 f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2020.http://tede.unioeste.br/handle/tede/4896Chicken is an animal protein most consumed in Brazil, which is a leader in the world ranking of exports of this food. Its high consumption must be low price and high nutritional value, however, it is a vehicle for pathogenic microorganisms such as Salmonella spp. Antimicrobial agents have been used in agriculture for many years to control the proliferation of these microorganisms and this favored prolonged stay or surgery of resistance mechanisms in strains of Salmonella spp. Therefore, the aim of the present study was to isolate and characterize Salmonella spp. from cuts of chickens marketed in the city of Francisco Beltrão - Paraná and verify the front resistance profile for antimicrobials used in human medicine. 40 samples of chicken cuts were purchased from supermarkets in Francisco Beltrão - PR. The microbiological analyzes followed the stages of preenrichment, selective enrichment and differential plating. The suspected colonies were subjected to biochemical tests carried out in a Kit for the identification of enterobacteria (NEWPROV). Four colonies from each sample were selected with Salmonella spp. biochemical behavior, totaling 28 isolates, to perform the antimicrobial sensitivity test. Samples that showed resistance to ceftriaxone were subjected to extended spectrum beta lactamases. All isolates subjected to antimicrobial susceptibility testing demonstrated sensitivity to meropenem, whereas 100% of the isolates were resistant to nalidixic acid, a high resistance to beta lactam antimicrobials was also observed. Seventeen different resistance profiles were found, with 42.85% showing a multi-resistance profile and 21.42% of the isolates showing a phenotype for ESBL production. The frequency of batches contaminated with Salmonella spp. of 17.5% in chicken cuts destined for commercialization, points to the importance of proper cooking and handling of these foods for the prevention of salmonellosis outbreaks. The high resistance to antimicrobials and their potential for dissemination found in this study is a worrying fact, as it puts at risk the treatment of infections caused by Salmonella spp.O frango é a proteína animal mais consumida no Brasil e líder no ranking mundial de exportação deste alimento. Seu elevado consumo se deve ao baixo preço e alto valor nutricional, no entanto, é veículo para microrganismos patogênicos como Salmonella spp. Os agentes antimicrobianos foram utilizados na avicultura durante muitos anos para controlar a proliferação desses microrganismos e essa exposição prolongada favoreceu o surgimento de mecanismos de resistência em cepas de Salmonella spp. Sendo assim, o objetivo do presente trabalho foi isolar e caracterizar bactérias do gênero Salmonella spp. a partir de cortes de frangos comercializados na cidade de Francisco Beltrão – Paraná, e verificar o perfil de resistência dos isolados frente aos antimicrobianos utilizados na medicina humana. Foram adquiridas 40 amostras de cortes de frango dos supermercados de Francisco Beltrão –PR. As análises microbiológicas seguiram as etapas de pré-enriquecimento, enriquecimento seletivo e plaqueamento diferencial. As colônias suspeitas foram submetidas a provas bioquímicas realizadas em Kit para identificação de enterobactérias (NEWPROV). Foram selecionadas, quatro colônias de cada amostra com comportamento bioquímico de Salmonella spp, totalizando 28 isolados para a realização do teste de sensibilidade a antimicrobianos. As amostras que apresentaram resistência a ceftriaxona foram submetidas a pesquisa de beta-lactamases de espectro estendido. Todos os isolados submetidos a teste de sensibilidade a antimicrobianos demonstraram sensibilidade ao meropenem, ao passo que 100% dos isolados foram resistentes ao ácido nalidíxico. Foi observado uma elevada resistência aos antimicrobianos beta-lactâmicos. Foram encontrados 17 perfis diferentes de resistência, sendo que 42,85% apresentaram perfil de multirresistência e 21,42% dos isolados apresentaram fenótipo para produção de Beta-Lactamase de Espectro Estendido (ESBL). A frequência de lotes contaminados com Salmonella spp. de 17,5% em cortes de frangos destinados a comercialização, aponta para a importância de uma adequada cocção e manipulação desses alimentos para a prevenção de surtos de salmonelose. A elevada resistência aos antimicrobianos e seu potencial de disseminação encontrados nesse estudo é um dado preocupante, pois coloca em risco o tratamento de infecções causadas por Salmonella spp.Submitted by Almir Squinsani (almir.squinsani@unioeste.br) on 2020-09-01T17:09:41Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Ana_Silva_2020.pdf: 1313305 bytes, checksum: 2feb206af33b7f5f5616026e1fe91431 (MD5)Made available in DSpace on 2020-09-01T17:09:41Z (GMT). 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dc.title.por.fl_str_mv Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
dc.title.alternative.eng.fl_str_mv Isolation, characterization and resistance profile of salmonella spp. isolated from chicken cuts
title Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
spellingShingle Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
Silva, Ana Carolina Pereira da
Antimicrobianos
Microbiota
Microrganismos
Avicultura
Poultry
Antimocrobials
Microbiota
Microorganisms
CIÊNCIAS DA SAÚDE
title_short Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
title_full Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
title_fullStr Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
title_full_unstemmed Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
title_sort Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos
author Silva, Ana Carolina Pereira da
author_facet Silva, Ana Carolina Pereira da
author_role author
dc.contributor.advisor1.fl_str_mv Casaril, Kérley Braga Pereira Bento
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/6429832392023465
dc.contributor.referee1.fl_str_mv Casaril, Kérley Braga Pereira Bento
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6429832392023465
dc.contributor.referee2.fl_str_mv Lucio, Léia Carolina
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/9357029990194108
dc.contributor.referee3.fl_str_mv Benedetti, Volmir Pitt
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/8681216826241489
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9205567887145256
dc.contributor.author.fl_str_mv Silva, Ana Carolina Pereira da
contributor_str_mv Casaril, Kérley Braga Pereira Bento
Casaril, Kérley Braga Pereira Bento
Lucio, Léia Carolina
Benedetti, Volmir Pitt
dc.subject.por.fl_str_mv Antimicrobianos
Microbiota
Microrganismos
Avicultura
Poultry
topic Antimicrobianos
Microbiota
Microrganismos
Avicultura
Poultry
Antimocrobials
Microbiota
Microorganisms
CIÊNCIAS DA SAÚDE
dc.subject.eng.fl_str_mv Antimocrobials
Microbiota
Microorganisms
dc.subject.cnpq.fl_str_mv CIÊNCIAS DA SAÚDE
description Chicken is an animal protein most consumed in Brazil, which is a leader in the world ranking of exports of this food. Its high consumption must be low price and high nutritional value, however, it is a vehicle for pathogenic microorganisms such as Salmonella spp. Antimicrobial agents have been used in agriculture for many years to control the proliferation of these microorganisms and this favored prolonged stay or surgery of resistance mechanisms in strains of Salmonella spp. Therefore, the aim of the present study was to isolate and characterize Salmonella spp. from cuts of chickens marketed in the city of Francisco Beltrão - Paraná and verify the front resistance profile for antimicrobials used in human medicine. 40 samples of chicken cuts were purchased from supermarkets in Francisco Beltrão - PR. The microbiological analyzes followed the stages of preenrichment, selective enrichment and differential plating. The suspected colonies were subjected to biochemical tests carried out in a Kit for the identification of enterobacteria (NEWPROV). Four colonies from each sample were selected with Salmonella spp. biochemical behavior, totaling 28 isolates, to perform the antimicrobial sensitivity test. Samples that showed resistance to ceftriaxone were subjected to extended spectrum beta lactamases. All isolates subjected to antimicrobial susceptibility testing demonstrated sensitivity to meropenem, whereas 100% of the isolates were resistant to nalidixic acid, a high resistance to beta lactam antimicrobials was also observed. Seventeen different resistance profiles were found, with 42.85% showing a multi-resistance profile and 21.42% of the isolates showing a phenotype for ESBL production. The frequency of batches contaminated with Salmonella spp. of 17.5% in chicken cuts destined for commercialization, points to the importance of proper cooking and handling of these foods for the prevention of salmonellosis outbreaks. The high resistance to antimicrobials and their potential for dissemination found in this study is a worrying fact, as it puts at risk the treatment of infections caused by Salmonella spp.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-01T17:09:41Z
dc.date.issued.fl_str_mv 2020-03-03
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dc.identifier.citation.fl_str_mv SILVA, Ana Carolina Pereira da. Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos. 2020. 71 f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2020.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br/handle/tede/4896
identifier_str_mv SILVA, Ana Carolina Pereira da. Isolamento, caracterização e perfil de resistência de salmonella spp. isoladas de cortes de frangos. 2020. 71 f. Dissertação (Mestrado em Ciências Aplicadas à Saúde) - Universidade Estadual do Oeste do Paraná, Francisco Beltrão, 2020.
url http://tede.unioeste.br/handle/tede/4896
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Francisco Beltrão
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dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
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