Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético

Detalhes bibliográficos
Autor(a) principal: Oliveira, Vanilva Pereira de
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: https://tede.unioeste.br/handle/tede/7192
Resumo: One of the herbicides most commonly used in agriculture is 2,4 dichlorophenoxyacetic acid, its indiscriminate use has generated an increase in water pollution, causing great environmental concerns. The objective of the present study was to determine the toxicological effects of the herbicide 2,4-D in juveniles of Rhamdia quelen (jundiá). To this end, 96 specimens of Rhamdia quelen were subjected to an acute test (96h) of exposure to the 2.4 D herbicide at increasing concentrations of 0.0; 15; 30 and 45 mg / L. For each concentration, 4 replicates were made, with 6 specimens each. After 96 hours, the animals were weighed, measured and anesthetized for blood collection for hematological analysis, beingquantified: the number of erythrocytes, leukocytes, thrombocytes and monocytes. Then, the animals were euthanized and laparatomized to collect the muscles and livers for histomorphometric and biochemical analyzes. For histomorphometry, liver and muscle were isolated clean and weighed and submitted to routine histological techniques, stained and evaluated for the hepatosomatic index (IHS) and condition factor (HR). A liver fragment was used for enzymatic analysis of LPO, AChE and PT. Hematological analyzes showed that 2,4-D causes changes in the number of leukocytes, monocytes and thrombocytes, in all concentrations compared to the control group (not exposed). The mean value of erythrocytes was 1.6x106µL-1. 2,4-D did not significantly change the diameter of muscle fibers (p≤0.05), but the herbicide at concentrations of 30 and 45mg / L reduced and increased, respectively, the number of hepatocytes when compared to the control group (not exposed). The activity of hepatic OLP was not altered by 2,4-D at any concentration used. Similarly, there was no evidence of inhibition of hepatic acetylcholinesterase (AChE) activity in animals exposed to 2,4-D (p≤0.05). However, at concentrations of 30 and 45 mg/L of 2,4-D, a reduction in total protein was observed. The changes found in Rhamdia quelen exposed to 2,4-D indicate that, acutely, exposure to the herbicide mainly promotes liver and hematological damage, indicating greater vulnerability of these tissues to the herbicide in the short term. Chronic exposure is likely to increase damage and cause further changes in other tissues. The results obtained in this work will serve as subsidies for further research with toxicological tests with the herbicide 2,4-D, and will assist in the understanding of morphometric, histological, hematological and biochemical behaviors for juveniles of the species Rhamdia quelen, exposed to acute contamination by the 2,4-D herbicide.
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spelling Baumgartner, Gilmarhttp://lattes.cnpq.br/4429292734049977Grassiolli, Sabrinahttp://lattes.cnpq.br/1379417550389891Sanches, Paulo Vanderleihttp://lattes.cnpq.br/3472813769118299Piana, Pitágoras Augustohttp://lattes.cnpq.br/4575333508754234Silva, Geuza Cantanhêde dahttp://lattes.cnpq.br/3114762977907586Oliveira, Luciano Caetano dehttp://lattes.cnpq.br/4536577212357447Baumgartner, Gilmarhttp://lattes.cnpq.br/4429292734049977http://lattes.cnpq.br/9844982099530523Oliveira, Vanilva Pereira de2024-05-08T17:59:03Z2021-05-31OLIVEIRA, Vanilva Pereira de. Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético. 2021. 57 f. Tese (Doutorado em Recursos Pesqueiros e Engenharia de Pesca) - Universidade Estadual do Oeste do Paraná, Toledo, 2021.https://tede.unioeste.br/handle/tede/7192One of the herbicides most commonly used in agriculture is 2,4 dichlorophenoxyacetic acid, its indiscriminate use has generated an increase in water pollution, causing great environmental concerns. The objective of the present study was to determine the toxicological effects of the herbicide 2,4-D in juveniles of Rhamdia quelen (jundiá). To this end, 96 specimens of Rhamdia quelen were subjected to an acute test (96h) of exposure to the 2.4 D herbicide at increasing concentrations of 0.0; 15; 30 and 45 mg / L. For each concentration, 4 replicates were made, with 6 specimens each. After 96 hours, the animals were weighed, measured and anesthetized for blood collection for hematological analysis, beingquantified: the number of erythrocytes, leukocytes, thrombocytes and monocytes. Then, the animals were euthanized and laparatomized to collect the muscles and livers for histomorphometric and biochemical analyzes. For histomorphometry, liver and muscle were isolated clean and weighed and submitted to routine histological techniques, stained and evaluated for the hepatosomatic index (IHS) and condition factor (HR). A liver fragment was used for enzymatic analysis of LPO, AChE and PT. Hematological analyzes showed that 2,4-D causes changes in the number of leukocytes, monocytes and thrombocytes, in all concentrations compared to the control group (not exposed). The mean value of erythrocytes was 1.6x106µL-1. 2,4-D did not significantly change the diameter of muscle fibers (p≤0.05), but the herbicide at concentrations of 30 and 45mg / L reduced and increased, respectively, the number of hepatocytes when compared to the control group (not exposed). The activity of hepatic OLP was not altered by 2,4-D at any concentration used. Similarly, there was no evidence of inhibition of hepatic acetylcholinesterase (AChE) activity in animals exposed to 2,4-D (p≤0.05). However, at concentrations of 30 and 45 mg/L of 2,4-D, a reduction in total protein was observed. The changes found in Rhamdia quelen exposed to 2,4-D indicate that, acutely, exposure to the herbicide mainly promotes liver and hematological damage, indicating greater vulnerability of these tissues to the herbicide in the short term. Chronic exposure is likely to increase damage and cause further changes in other tissues. The results obtained in this work will serve as subsidies for further research with toxicological tests with the herbicide 2,4-D, and will assist in the understanding of morphometric, histological, hematological and biochemical behaviors for juveniles of the species Rhamdia quelen, exposed to acute contamination by the 2,4-D herbicide.Um dos herbicidas mais comumente usados na agricultura é o ácido 2,4 diclorofenoxiacético, seu uso indiscriminado tem gerado aumento da poluição das águas, causando grandes preocupações ambientais. O objetivo do presente estudo foi determinar os efeitos toxicológicos do herbicida 2,4-D em juvenis de Rhamdia quelen (jundiá). Para tal, 96 exemplares de Rhamdia quelen foram submetidos a um teste agudo (96h) de exposição ao herbicida 2,4 D nas concentrações crescentes de 0,0; 15; 30 e 45 mg/L. Para cada concentração foram feitas 4 réplicas, com 6 exemplares cada. Após 96 horas, os animais foram pesados, medidos e anestesiados para a coleta de sangue para as análises hematológicas, sendo quantificados: o número de eritrócitos, leucócitos, trombócitos e monócitos. Em seguida, os animais foram eutanasiados e laparatomizados para a coleta dos músculos e fígados destinados às análises histomorfométricas e bioquímicas. Para histomorfometria, fígado e músculo foram isolados limpos e pesados sendo submetidos a técnicas histológicas de rotina, corados e avaliados quanto ao Índice hepatossomático (IHS) e Fator de condição (FC). Um fragmento hepático, foi destinado análise enzimática da LPO, AChE e PT. As análises hematológicas mostraram que o 2,4-D provoca alteração no número de leucócitos, monócitos e trombócitos, em todas as concentrações em comparação ao grupo controle (não exposto). Já o valor médio de eritrócitos foi de 1,6x106µL-1. O 2,4-D não alterou significativamente o diâmetro das fibras musculares (p≤0,05), porém o herbicida nas concentrações de 30 e 45mg/L reduziu e aumentou, respectivamente, o número de hepatócitos quando comparado ao grupo controle (não exposto). A atividade da LPO hepática não foi alterada pelo 2,4-D em nenhuma concentração utilizadas. Similarmente, não foi evidenciada inibição da atividade da Acetilcolinesterase (AChE) hepática nos animais expostos ao 2,4-D (p≤0,05). Todavia, nas concentrações de 30 e 45mg/L do 2,4-D foi observada redução da proteína total. As alterações encontradas em Rhamdia quelen expostos a 2,4-D indicam que, agudamente a exposição ao herbicida promove principalmente danos hepáticos e hematológicos, indicando maior vulnerabilidade destes tecidos ao herbicida a curto prazo. É provável que a exposição crônica possa elevar o dano e provocar maiores alterações em outros tecidos. Os resultados obtidos neste trabalho servirão de subsídios para novas pesquisas com ensaios toxicológicos com o herbicida 2,4-D, e irão auxiliar na compreensão dos comportamentos morfométricos, histológicos, hematológicos e bioquímicos para os juvenis da espécie Rhamdia quelen, expostos a uma contaminação aguda pelo herbicida 2,4-D.Submitted by Marilene Donadel (marilene.donadel@unioeste.br) on 2024-05-08T17:59:03Z No. of bitstreams: 1 Vanilva_Oliveira_2021.pdf: 1235002 bytes, checksum: 653450758a8fcb79c148f19c5fe4eaad (MD5)Made available in DSpace on 2024-05-08T17:59:03Z (GMT). No. of bitstreams: 1 Vanilva_Oliveira_2021.pdf: 1235002 bytes, checksum: 653450758a8fcb79c148f19c5fe4eaad (MD5) Previous issue date: 2021-05-31application/pdfpor-2624803687637593200500Universidade Estadual do Oeste do ParanáToledoPrograma de Pós-Graduação em Recursos Pesqueiros e Engenharia de PescaUNIOESTEBrasilCentro de Engenharias e Ciências Exatashttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessJundiáHistologiaHematologiaLipoperoxidaçãoAcetilcolinesteraseHistologyHematologyLipoperoxidationAcetylcholinesteraseCIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCAAvaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacéticoToxicological evaluation in juveniles of Rhamdia quelen subjected to different concentrations of the herbicide 2,4 dichlorophenoxyacetic acidinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis634168548598223434600600600-7734402124082146922-6131750198709519811reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALVanilva_Oliveira_2021.pdfVanilva_Oliveira_2021.pdfapplication/pdf1235002http://tede.unioeste.br:8080/tede/bitstream/tede/7192/2/Vanilva_Oliveira_2021.pdf653450758a8fcb79c148f19c5fe4eaadMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/7192/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/71922024-05-08 14:59:03.801oai:tede.unioeste.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2024-05-08T17:59:03Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
dc.title.alternative.eng.fl_str_mv Toxicological evaluation in juveniles of Rhamdia quelen subjected to different concentrations of the herbicide 2,4 dichlorophenoxyacetic acid
title Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
spellingShingle Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
Oliveira, Vanilva Pereira de
Jundiá
Histologia
Hematologia
Lipoperoxidação
Acetilcolinesterase
Histology
Hematology
Lipoperoxidation
Acetylcholinesterase
CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
title_short Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
title_full Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
title_fullStr Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
title_full_unstemmed Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
title_sort Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético
author Oliveira, Vanilva Pereira de
author_facet Oliveira, Vanilva Pereira de
author_role author
dc.contributor.advisor1.fl_str_mv Baumgartner, Gilmar
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4429292734049977
dc.contributor.advisor-co1.fl_str_mv Grassiolli, Sabrina
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/1379417550389891
dc.contributor.referee1.fl_str_mv Sanches, Paulo Vanderlei
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3472813769118299
dc.contributor.referee2.fl_str_mv Piana, Pitágoras Augusto
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/4575333508754234
dc.contributor.referee3.fl_str_mv Silva, Geuza Cantanhêde da
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/3114762977907586
dc.contributor.referee4.fl_str_mv Oliveira, Luciano Caetano de
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/4536577212357447
dc.contributor.referee5.fl_str_mv Baumgartner, Gilmar
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/4429292734049977
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9844982099530523
dc.contributor.author.fl_str_mv Oliveira, Vanilva Pereira de
contributor_str_mv Baumgartner, Gilmar
Grassiolli, Sabrina
Sanches, Paulo Vanderlei
Piana, Pitágoras Augusto
Silva, Geuza Cantanhêde da
Oliveira, Luciano Caetano de
Baumgartner, Gilmar
dc.subject.por.fl_str_mv Jundiá
Histologia
Hematologia
Lipoperoxidação
Acetilcolinesterase
topic Jundiá
Histologia
Hematologia
Lipoperoxidação
Acetilcolinesterase
Histology
Hematology
Lipoperoxidation
Acetylcholinesterase
CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
dc.subject.eng.fl_str_mv Histology
Hematology
Lipoperoxidation
Acetylcholinesterase
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
description One of the herbicides most commonly used in agriculture is 2,4 dichlorophenoxyacetic acid, its indiscriminate use has generated an increase in water pollution, causing great environmental concerns. The objective of the present study was to determine the toxicological effects of the herbicide 2,4-D in juveniles of Rhamdia quelen (jundiá). To this end, 96 specimens of Rhamdia quelen were subjected to an acute test (96h) of exposure to the 2.4 D herbicide at increasing concentrations of 0.0; 15; 30 and 45 mg / L. For each concentration, 4 replicates were made, with 6 specimens each. After 96 hours, the animals were weighed, measured and anesthetized for blood collection for hematological analysis, beingquantified: the number of erythrocytes, leukocytes, thrombocytes and monocytes. Then, the animals were euthanized and laparatomized to collect the muscles and livers for histomorphometric and biochemical analyzes. For histomorphometry, liver and muscle were isolated clean and weighed and submitted to routine histological techniques, stained and evaluated for the hepatosomatic index (IHS) and condition factor (HR). A liver fragment was used for enzymatic analysis of LPO, AChE and PT. Hematological analyzes showed that 2,4-D causes changes in the number of leukocytes, monocytes and thrombocytes, in all concentrations compared to the control group (not exposed). The mean value of erythrocytes was 1.6x106µL-1. 2,4-D did not significantly change the diameter of muscle fibers (p≤0.05), but the herbicide at concentrations of 30 and 45mg / L reduced and increased, respectively, the number of hepatocytes when compared to the control group (not exposed). The activity of hepatic OLP was not altered by 2,4-D at any concentration used. Similarly, there was no evidence of inhibition of hepatic acetylcholinesterase (AChE) activity in animals exposed to 2,4-D (p≤0.05). However, at concentrations of 30 and 45 mg/L of 2,4-D, a reduction in total protein was observed. The changes found in Rhamdia quelen exposed to 2,4-D indicate that, acutely, exposure to the herbicide mainly promotes liver and hematological damage, indicating greater vulnerability of these tissues to the herbicide in the short term. Chronic exposure is likely to increase damage and cause further changes in other tissues. The results obtained in this work will serve as subsidies for further research with toxicological tests with the herbicide 2,4-D, and will assist in the understanding of morphometric, histological, hematological and biochemical behaviors for juveniles of the species Rhamdia quelen, exposed to acute contamination by the 2,4-D herbicide.
publishDate 2021
dc.date.issued.fl_str_mv 2021-05-31
dc.date.accessioned.fl_str_mv 2024-05-08T17:59:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Vanilva Pereira de. Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético. 2021. 57 f. Tese (Doutorado em Recursos Pesqueiros e Engenharia de Pesca) - Universidade Estadual do Oeste do Paraná, Toledo, 2021.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/7192
identifier_str_mv OLIVEIRA, Vanilva Pereira de. Avaliação toxicológica em juvenis de Rhamdia quelen submetidos a diferentes concentrações do herbicida 2,4 diclorofenoxiacético. 2021. 57 f. Tese (Doutorado em Recursos Pesqueiros e Engenharia de Pesca) - Universidade Estadual do Oeste do Paraná, Toledo, 2021.
url https://tede.unioeste.br/handle/tede/7192
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 634168548598223434
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -7734402124082146922
dc.relation.cnpq.fl_str_mv -6131750198709519811
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Toledo
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