Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar

Detalhes bibliográficos
Autor(a) principal: Dalla Costa, Diely Melonari
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: http://tede.unioeste.br/handle/tede/5452
Resumo: Probiotics are living microrganisms that, when administered in appropriate amounts, confer benefits to the health of the host. Among several applications the regulation of intestinal microbiota stands out. They present antibacterial activity in front of several Gram-positive and negative species through some action mechanisms, being the main suggested the capacity of production of antimicrobial substances, as the bacteriocins. Bifidobacterium is a probiotic bacterial genus present in the microbiota of some human sites, being the species B. animalis subsp. lactis one of the known strains with good results of its probiotic action, but little explored in relation to the antibacterial activity. The growing reports of resistance, mainly nosocomial, and the search for new antimicrobial agents justify the realization of this work, which aimed to evaluate the activity of the cell-free supernatant (CFS) of B. animalis subsp. lactis INL1 culture against methylicin-resistant Staphylococcus aureus (MRSA), carbapenemase producing Klebsiella pneumoniae and extended spectrum beta-lactamase producing Escherichia coli (ESBL) isolated from hospital environment. The CFS of the probiotic strain was tested using three methods. For all, pathogenic microorganisms were prepared in 0.89% saline suspension, adjusted to McFarland's 0.5 scale. By diffusion on agar, the suspension was sown in plate and 40 µL of the supernatant was added to the wells made in the soft agar in order to allow the diffusion of CFS through the medium. In the disc-diffusion methodology, the CFS in test was applied on previously sterilized discs, in the same volume of 40 µL, also on plates with previous sowing of the adjusted solutions. For both were tested together with negative control wells and discs with 0.89% sterile saline solution. For the microdilution in the broth, the antimicrobial activity was evaluated using 100 µL of CFS on the same volume of each microbial suspension. They were also applied in the microplate wells with sterility control (only with Brain Heart Infusion broth - BHI) and growth control, (BHI broth + bacterial suspension). The results showed that CFS was not able to inhibit the growth of pathogenic strains and there is a need for further studies to optimize the growth of B. animalis subsp. lactis INL1 that favor the production of bacteriocins and allow better investigation of the antimicrobial action in vitro.
id UNIOESTE-1_87bdb03aa73705855873ee57c992a890
oai_identifier_str oai:tede.unioeste.br:tede/5452
network_acronym_str UNIOESTE-1
network_name_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
repository_id_str
spelling Ayala, Thais Sopranihttp://lattes.cnpq.br/5576750114963342Fariña, Luciana Oliveira dehttp://lattes.cnpq.br/2043990245681647Ayala, Thais Sopranihttp://lattes.cnpq.br/5576750114963342Menolli , Rafael Andradehttp://lattes.cnpq.br/2119646769798898Bella, Leonardo Mendeshttp://lattes.cnpq.br/7699259360505008Fariña, Luciana Oliveira dehttp://lattes.cnpq.br/2043990245681647http://lattes.cnpq.br/4782101887450249Dalla Costa, Diely Melonari2021-07-06T13:59:14Z2020-10-27DALLA COSTA, Diely Melonari. Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar. 2020. 37 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel - PR.http://tede.unioeste.br/handle/tede/5452Probiotics are living microrganisms that, when administered in appropriate amounts, confer benefits to the health of the host. Among several applications the regulation of intestinal microbiota stands out. They present antibacterial activity in front of several Gram-positive and negative species through some action mechanisms, being the main suggested the capacity of production of antimicrobial substances, as the bacteriocins. Bifidobacterium is a probiotic bacterial genus present in the microbiota of some human sites, being the species B. animalis subsp. lactis one of the known strains with good results of its probiotic action, but little explored in relation to the antibacterial activity. The growing reports of resistance, mainly nosocomial, and the search for new antimicrobial agents justify the realization of this work, which aimed to evaluate the activity of the cell-free supernatant (CFS) of B. animalis subsp. lactis INL1 culture against methylicin-resistant Staphylococcus aureus (MRSA), carbapenemase producing Klebsiella pneumoniae and extended spectrum beta-lactamase producing Escherichia coli (ESBL) isolated from hospital environment. The CFS of the probiotic strain was tested using three methods. For all, pathogenic microorganisms were prepared in 0.89% saline suspension, adjusted to McFarland's 0.5 scale. By diffusion on agar, the suspension was sown in plate and 40 µL of the supernatant was added to the wells made in the soft agar in order to allow the diffusion of CFS through the medium. In the disc-diffusion methodology, the CFS in test was applied on previously sterilized discs, in the same volume of 40 µL, also on plates with previous sowing of the adjusted solutions. For both were tested together with negative control wells and discs with 0.89% sterile saline solution. For the microdilution in the broth, the antimicrobial activity was evaluated using 100 µL of CFS on the same volume of each microbial suspension. They were also applied in the microplate wells with sterility control (only with Brain Heart Infusion broth - BHI) and growth control, (BHI broth + bacterial suspension). The results showed that CFS was not able to inhibit the growth of pathogenic strains and there is a need for further studies to optimize the growth of B. animalis subsp. lactis INL1 that favor the production of bacteriocins and allow better investigation of the antimicrobial action in vitro.Probióticos são microrganismos vivos que, quando administrados em quantidades adequadas, conferem benefícios à saúde do hospedeiro. Entre várias aplicações destaca-se a regulação da microbiota intestinal. Apresentam atividade antibacteriana frente diversas espécies Gram-positivas e negativas através de alguns mecanismos de ação, sendo o principal sugerido a capacidade de produção de substâncias antimicrobianas, como as bacteriocinas. Bifidobacterium é um gênero bacteriano probiótico presente na microbiota de alguns sítios humanos, sendo a espécie B. animalis subsp. lactis uma das cepas conhecidas com bons resultados de sua ação probiótica, mas pouco explorada em relação à atividade antibacteriana. Os crescentes relatos de resistência, principalmente nosocomiais, e a busca por novos agentes antimicrobianos justificam a realização deste trabalho, que objetivou avaliar a atividade do sobrenadante livre de células (CFS) da cultura de B. animalis subsp. lactis INL1 contra Staphylococcus aureus resistente a metilicina (MRSA), Klebsiella pneumoniae produtora de carbapenemase e Escherichia coli produtora de beta lactamase de espectro estendido (ESBL) isoladas de ambiente hospitalar. O CFS da cepa probiótica foi testado utilizando três métodos. Para todos, os microrganismos patógenos foram preparados em suspensões de solução salina a 0,89%, ajustados à escala 0,5 de McFarland. Por difusão em ágar, a suspensão foi semeada em placa e 40 µL do sobrenadante foram adicionados à poços confeccionados no soft ágar a fim de permitir a difusão do CFS pelo meio. Na metodologia com disco-difusão, o CFS em teste foi aplicado sobre discos previamente esterilizados, no mesmo volume de 40 µL, também sobre placas com semeadura prévia das soluções ajustadas. Para ambos foram testados em conjunto poços e discos de controle negativo com solução salina a 0,89% estéril. Já na microdiluição em caldo, a atividade antimicrobiana foi avaliada com a utilização de 100 µL do CFS sobre mesmo volume de cada suspensão microbiana. Também foram aplicados na microplaca poços com controle de esterilidade (apenas com caldo Brain Heart Infusion - BHI) e controle de crescimento, (caldo BHI + suspensão bacteriana). Os resultados evidenciaram que o CFS não foi capaz de inibir o crescimento das cepas patógenas e ensejam a necessidade de estudos posteriores que visem a otimização do crescimento da B. animalis subsp. lactis INL1 que favoreçam a produção de bacteriocinas e permitam melhor investigar a ação antimicrobiana in vitro.Submitted by Neusa Fagundes (neusa.fagundes@unioeste.br) on 2021-07-06T13:59:14Z No. of bitstreams: 2 Diely_MDallaCosta2020.pdf: 720587 bytes, checksum: ca3a3a00ddc180e6d417475653ea9fbb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2021-07-06T13:59:14Z (GMT). No. of bitstreams: 2 Diely_MDallaCosta2020.pdf: 720587 bytes, checksum: ca3a3a00ddc180e6d417475653ea9fbb (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2020-10-27application/pdfpor6588633818200016417500Universidade Estadual do Oeste do ParanáCascavelPrograma de Pós-Graduação em Ciências FarmacêuticasUNIOESTEBrasilCentro de Ciências Médicas e Farmacêuticashttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessProbióticosBifidobactériaResistência bacterianaSusceptibilidadeAntagonismoProbioticsBifidobacteriaBacterial resistanceSusceptibilityAntagonismCIENCIAS DA SAUDE::FARMACIAAtividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalarBifidobacterium animalis subsp. lactis INL1 activity on pathogenic bacteria isolated in hospital environmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7878055067573953101600600600-89404397133878492676997636413449754996reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALDiely_MDallaCosta2020.pdfDiely_MDallaCosta2020.pdfapplication/pdf720587http://tede.unioeste.br:8080/tede/bitstream/tede/5452/5/Diely_MDallaCosta2020.pdfca3a3a00ddc180e6d417475653ea9fbbMD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://tede.unioeste.br:8080/tede/bitstream/tede/5452/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/5452/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.unioeste.br:8080/tede/bitstream/tede/5452/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://tede.unioeste.br:8080/tede/bitstream/tede/5452/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/54522021-07-06 10:59:14.934oai:tede.unioeste.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2021-07-06T13:59:14Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false
dc.title.por.fl_str_mv Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
dc.title.alternative.eng.fl_str_mv Bifidobacterium animalis subsp. lactis INL1 activity on pathogenic bacteria isolated in hospital environment
title Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
spellingShingle Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
Dalla Costa, Diely Melonari
Probióticos
Bifidobactéria
Resistência bacteriana
Susceptibilidade
Antagonismo
Probiotics
Bifidobacteria
Bacterial resistance
Susceptibility
Antagonism
CIENCIAS DA SAUDE::FARMACIA
title_short Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
title_full Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
title_fullStr Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
title_full_unstemmed Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
title_sort Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar
author Dalla Costa, Diely Melonari
author_facet Dalla Costa, Diely Melonari
author_role author
dc.contributor.advisor1.fl_str_mv Ayala, Thais Soprani
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5576750114963342
dc.contributor.advisor-co1.fl_str_mv Fariña, Luciana Oliveira de
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2043990245681647
dc.contributor.referee1.fl_str_mv Ayala, Thais Soprani
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5576750114963342
dc.contributor.referee2.fl_str_mv Menolli , Rafael Andrade
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2119646769798898
dc.contributor.referee3.fl_str_mv Bella, Leonardo Mendes
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/7699259360505008
dc.contributor.referee4.fl_str_mv Fariña, Luciana Oliveira de
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/2043990245681647
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4782101887450249
dc.contributor.author.fl_str_mv Dalla Costa, Diely Melonari
contributor_str_mv Ayala, Thais Soprani
Fariña, Luciana Oliveira de
Ayala, Thais Soprani
Menolli , Rafael Andrade
Bella, Leonardo Mendes
Fariña, Luciana Oliveira de
dc.subject.por.fl_str_mv Probióticos
Bifidobactéria
Resistência bacteriana
Susceptibilidade
Antagonismo
topic Probióticos
Bifidobactéria
Resistência bacteriana
Susceptibilidade
Antagonismo
Probiotics
Bifidobacteria
Bacterial resistance
Susceptibility
Antagonism
CIENCIAS DA SAUDE::FARMACIA
dc.subject.eng.fl_str_mv Probiotics
Bifidobacteria
Bacterial resistance
Susceptibility
Antagonism
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::FARMACIA
description Probiotics are living microrganisms that, when administered in appropriate amounts, confer benefits to the health of the host. Among several applications the regulation of intestinal microbiota stands out. They present antibacterial activity in front of several Gram-positive and negative species through some action mechanisms, being the main suggested the capacity of production of antimicrobial substances, as the bacteriocins. Bifidobacterium is a probiotic bacterial genus present in the microbiota of some human sites, being the species B. animalis subsp. lactis one of the known strains with good results of its probiotic action, but little explored in relation to the antibacterial activity. The growing reports of resistance, mainly nosocomial, and the search for new antimicrobial agents justify the realization of this work, which aimed to evaluate the activity of the cell-free supernatant (CFS) of B. animalis subsp. lactis INL1 culture against methylicin-resistant Staphylococcus aureus (MRSA), carbapenemase producing Klebsiella pneumoniae and extended spectrum beta-lactamase producing Escherichia coli (ESBL) isolated from hospital environment. The CFS of the probiotic strain was tested using three methods. For all, pathogenic microorganisms were prepared in 0.89% saline suspension, adjusted to McFarland's 0.5 scale. By diffusion on agar, the suspension was sown in plate and 40 µL of the supernatant was added to the wells made in the soft agar in order to allow the diffusion of CFS through the medium. In the disc-diffusion methodology, the CFS in test was applied on previously sterilized discs, in the same volume of 40 µL, also on plates with previous sowing of the adjusted solutions. For both were tested together with negative control wells and discs with 0.89% sterile saline solution. For the microdilution in the broth, the antimicrobial activity was evaluated using 100 µL of CFS on the same volume of each microbial suspension. They were also applied in the microplate wells with sterility control (only with Brain Heart Infusion broth - BHI) and growth control, (BHI broth + bacterial suspension). The results showed that CFS was not able to inhibit the growth of pathogenic strains and there is a need for further studies to optimize the growth of B. animalis subsp. lactis INL1 that favor the production of bacteriocins and allow better investigation of the antimicrobial action in vitro.
publishDate 2020
dc.date.issued.fl_str_mv 2020-10-27
dc.date.accessioned.fl_str_mv 2021-07-06T13:59:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv DALLA COSTA, Diely Melonari. Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar. 2020. 37 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel - PR.
dc.identifier.uri.fl_str_mv http://tede.unioeste.br/handle/tede/5452
identifier_str_mv DALLA COSTA, Diely Melonari. Atividade de Bifidobacterium animalis subsp. lactis INL1 sobre bactérias patogênicas isoladas de ambiente hospitalar. 2020. 37 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, Cascavel - PR.
url http://tede.unioeste.br/handle/tede/5452
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv 7878055067573953101
dc.relation.confidence.fl_str_mv 600
600
600
dc.relation.department.fl_str_mv -8940439713387849267
dc.relation.cnpq.fl_str_mv 6997636413449754996
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Cascavel
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Farmacêuticas
dc.publisher.initials.fl_str_mv UNIOESTE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Centro de Ciências Médicas e Farmacêuticas
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
Cascavel
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTE
instname:Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron:UNIOESTE
instname_str Universidade Estadual do Oeste do Paraná (UNIOESTE)
instacron_str UNIOESTE
institution UNIOESTE
reponame_str Biblioteca Digital de Teses e Dissertações do UNIOESTE
collection Biblioteca Digital de Teses e Dissertações do UNIOESTE
bitstream.url.fl_str_mv http://tede.unioeste.br:8080/tede/bitstream/tede/5452/5/Diely_MDallaCosta2020.pdf
http://tede.unioeste.br:8080/tede/bitstream/tede/5452/2/license_url
http://tede.unioeste.br:8080/tede/bitstream/tede/5452/3/license_text
http://tede.unioeste.br:8080/tede/bitstream/tede/5452/4/license_rdf
http://tede.unioeste.br:8080/tede/bitstream/tede/5452/1/license.txt
bitstream.checksum.fl_str_mv ca3a3a00ddc180e6d417475653ea9fbb
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
bd3efa91386c1718a7f26a329fdcb468
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)
repository.mail.fl_str_mv biblioteca.repositorio@unioeste.br
_version_ 1811723443142393856