Infecção de células da ampola dos túbulos de MALPIGHI de Bombyx Mori pelo Alphabaculovirus

• Autor(a) principal: Santorum, Marilucia
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
• Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/650
• topic: Bicho-da-seda; BmNPV; Lepidoptera; Baculoviridae; Silkworm; BmNPV; Lepidoptera; Baculoviridae; CNPQ::CIENCIAS BIOLOGICAS

Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus Baculoviridae family, Alphabaculovirus genre, which infects the silkworm, Bombyx mori and causes nuclear polyhedrosis disease. A viral geographic isolated from BmNPV was identified in the state of Paraná, Brazil, and a number of organs and target tissues were identified, but there is no information about the infection of Malpighian tubules (MT). MT make up the excretory system of B. mori, acting in the elimination of toxic substances and electrolyte homeostasis. This study aimed to analyze the behavior on the susceptibility and cytopathology of MT B. mori to BmNPV. Therefore, hybrid B.mori caterpillars at 5º instar, were inoculated experimentally with a viral suspension of BmNPV. On different days post-inoculation (dpi), the 4° to 9° dpi, MT segments were collected, divided in regions of the ampullae, proximal, medial and distal; following the processing for light and electron microscopy transmission. Material control followed the same process that the inoculated. The results showed that B. mori MT showed variations in their morphology and susceptibility to BmNPV according to the analyzed region. Thus, the region of the ampullae in its transition area to the proximal, appeared susceptible to BmNPV, from 6° dpi, while other regions showed no evidence of infection by the pathogen. The transition area is a small region which is located at the end of the ampullae and the beginning of the proximal region of MT. The cellular morphology of simple lining epithelium showed continuous variation of pavement, characteristic of the ampullae, the cylindrical, in the transition area; already continuous with the proximal region of MT, this morphological change was abruptly. The cytopathology revealed in hypertrophic nucleus, viroplasm containing the nucleocapsid and viral polyhedra. At the end of the infectious cycle was observed cytolysis of infected cells, releasing polyhedra in the extracellular medium, both in hemocele as in MT lumen, a factor that may favor the transmission of the disease. Thus, infection of the ampullae in its transition area to the proximal region, will contribute to greater scientific knowledge of cytopathology aspects and targets of infection from this virus isolated geographical, for the establishment of its infectious cycle of the insect's body BmNPV