Influência da dieta de cafeteria sobre a morfologia e expressão de il6 e aquaporinas 1 e 9 no epidídimo de ratos Wistar

• Autor(a) principal: Mata, Patricia Terron Ghezzi da
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
• Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/644
• topic: Epididimo; inflamação; obesidade; aquaporinas; epitélio epididimário; interleucina; Epididymis; inflammation; obesity; aquaporins; epididimal epithelium; interleukin; CNPQ::CIENCIAS DA SAUDE::MEDICINA

The increase of fat tissue, in obesity, can be associated to physiological alterations, including important modifications in the concentration of circulating hormones, including the sexual steroid hormones. The epididymis, organ of the male genital system, is androgynous-dependent, therefore, susceptible to resulting modifications of sexual steroids hormones concentrations, which can compromise its main functions: transport, maturation, maintenance, protection and storage of the spermatozoids. The water transporting, in the epididymis, is essential to form the adequate luminal environment so the epididymal and spermatozoid related functions can happen; thus, alterations in aquaporins (AQPs), may intervene in the homeostasis of the duct and, consequently, negatively affect fertility. The accumulation of fat may increase the local temperature and alter the epididymal functions and, even, influence in the synthesis and secretion of cytokines such as the interleukin-6 (IL6), due to the chronicle inflammatory estate of low intensity as a consequence of obesity. The objective of this study was to evaluate the influence of the cafeteria diet over the morphology of the coating epithelium of the epididymal duct of rats and about the expression of IL6 and AQPs 1 and 9 in the mentioned epithelium. The analysis were made using tissue samples of the epididymis of Wistar rats of the groups control (CON, animals fed with the pattern diet) and cafeteria (CAF, animals fed with a cafeteria diet) treated throughout the experimental period of 24 weeks. The samples were submitted to a histological routine and immunohistochemical reactions for morphological, morphometric and the expression of IL6 and AQPs 1 and 9 analysis. The analysis evidenced a modification in the relative cellular distribution, with decrease of basal cells in the region of the epididymal body, and a significant increase of halo cells in the epididymal segments initial, head and tail of the CAF animals. The luminal diameter and the epithelial height didn t show significant differences between the animals. The intense expression of IL6 in the initial segment of the CAF animals was the outstanding result for this interleukin. The expression of AQP1 was altered in the CAF group animals, in general, with bigger expression in the vascular channels, mainly, the initial segment and the head; besides the bigger expression in the peritubular cells in the initial segment. The expression of AQP9 was altered only in the initial segment of the CAF group, which reactivity was average, while the reactivity was intense in this segment in the animals of the CON group. The results obtained indicate that the cafeteria diet promoted segment-specific alterations in the distribution of basal cell and halo and expression of IL6 and AQPs 1 and 9. These results allow us to deduce that the increase of body mass, induced by a cafeteria diet may promote alterations in the epididymal luminal environment with possible damage in the transport, maturation, maintenance, protection and storage of the spermatozoids. In order to enlighten the extension of the damage caused by the alterations resulting from the cafeteria diet, new studies such as epididymal biochemical alterations, ultrastructural analysis of the epididymal cell types and morphology and motility of the spermatozoids are necessary