Avaliação da terapia fotodinâmica em modelo experimental de artrite por paracoccidioides brasiliensis

• Autor(a) principal: Tomadon, Aniele
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
• Texto Completo: http://tede.unioeste.br:8080/tede/handle/tede/668
• topic: Paracoccidioidomicose, articulações, fotoquimioterapia, artrite séptica.; Paracoccidioidomycosis, joints, photochemotherapy, arthritis infectious; CNPQ::CIENCIAS BIOLOGICAS

Paracoccidioidomycosis (PCM), caused by the dimorphic fungus Paracoccidioides brasiliensis, is one of the most prevalent systemic mycosis in Latin America. Infection can occur accidentally through direct inoculation or mainly through inhalation of propagules of P. brasiliensis, which are installed initially in the lungs and can spread to organs and tissues of the host. In the acute form of the disease the major clinical manifestation is the febrile lymphoproliferative syndrome, occurring osteoarticular involvement; the same for the chronic form, in which 60% of cases, bone and joint changes usually treated for extended periods with conventional antifungal agents. Drug therapy is the most widely used to treatment the disease, however, high costs, poor adherence to treatment and adverse side effects, take the need to search for alternative therapies such as photodynamic (PDT), which has low cost and toxicity, local action and does not induce resistance to microorganisms. In vitro studies have shown positive results of PDT against various fungi, but there are no in vivo reports on its action in the control of P. brasiliensis. In this sense, the present study aimed to evaluate the PDT response in an experimental model of arthritis by P. brasiliensis. For this purpose, male Wistar rats (n = 42) were divided into groups submitted to inoculation with P. brasiliensis and PBS (control) in the right knee joint. After the development of arthritis, the mice were treated from the eighth to the eleventh day to the therapy: Groups L40 and L120 laser treated at dosages of 40 and 120 J/cm2, AM group treated with the photosensitizer methylene blue and PDT40 and PDT120 groups treated with PDT at doses of 40 and 120 J/cm2. The laser and AM therapies were used individually as a control for PDT. The groups were evaluated: edema, by measuring the lateral-lateral diameter of the joint; the intensity of arthritis, for histopathology and titration of anti-gp43 antibody by ELISA. The results showed that the edema formation was lower in the groups treated with PDT 40 and 120 J/cm2 (p = 0.8359 and p = 0.7167, respectively). The analysis of the intensity of arthritis as assessed by histopathology showed that the group treated with PDT 120 J/cm2 had the lowest morphological changes in the joint. Titration of anti-gp43 antibodies, showed the highest production of specific antibodies in the yeast group, but the differences were not significant when comparing the groups. Thus, the morphological evidence of normal tissue recovery aspects, the joint subjected to PDT 120 J/cm2, revealed the potential use of this therapy in reducing arthritis caused by P. brasiliensis