Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UNIOESTE |
Texto Completo: | http://tede.unioeste.br:8080/tede/handle/tede/1861 |
Resumo: | Oils and fats are essential foods to the human diet, nevertheless, the negative impact caused by its overconsumption is of great concern worldwide. Diacylglycerol rich oils have orga- noleptic features very similar to those found in conventional edible oils, however, these oils do not tend to accumulate on the body, even when consumed in high quantities, becoming a great resource in the fight against obesity. Sunflower oil is among the most consumed edible oils in the world, and also, is naturally one of the healthiest. This study s goal was to evalu- ate the influence of ultrasound on the enzymatic hydrolysis of sunflower oil with the enzyme phospolipase A1 (Lecitase Ultra) and use mathematical modeling to obtain simulations of production processes of a diacylglycerol rich oil. Ultrasound generates emulsions of water and oil, thus enhancing the system s interfacial area, and producing higher reaction rates compared to conventional enzymatic processes. Sunflower oil partial hydrolysis was carried in a solvent free environment, in 4 hour periods, with temperatures varying from 30 to 50 °C, enzyme/substrate fractions between 0,6 and 4,0% (m/m) and a fixed water/oil fraction of 5% (m/m). with this goal in mind, experimental designs were developed. Results showed optimal reaction conditions on 40 °C and 1,7% (m/m). Subsequently, fixing the optimal parameters, water/oil fraction was evaluated between 2 and 10% (m/m) in a 12 hour period in order to obtain reaction kinetics. Applying a mathematical model to the kinetic curves it was possible to demonstrate that, for the tested fractions, the 2% water/oil ratio is the most favorable to the production of diacylglycerol, and the maximum quantity of this product is obtained at 27 minutes of reaction time. |
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Cardozo Filho, LúcioCPF:10785177892http://lattes.cnpq.br/2710474728753403Silva, Edson Antônio Alves daCPF:64656675920http://lattes.cnpq.br/9304493875700070Teleken, Joel GustavoCPF:04253719996http://lattes.cnpq.br/6288735286919040Borba, Carlos EduardoCPF:03533333957http://lattes.cnpq.br/0750048720229101CPF:05374424998http://lattes.cnpq.br/0789820025444227Raizer, Eduardo2017-07-10T18:08:06Z2017-04-152015-02-24RAIZER, Eduardo. Use of ultrasound in diacylglycerol synthesis via enzymatic hydrolysis of sunflower oil. 2015. 69 f. Dissertação (Mestrado em Desenvolvimento de Processos) - Universidade Estadual do Oeste do Parana, Toledo, 2015.http://tede.unioeste.br:8080/tede/handle/tede/1861Oils and fats are essential foods to the human diet, nevertheless, the negative impact caused by its overconsumption is of great concern worldwide. Diacylglycerol rich oils have orga- noleptic features very similar to those found in conventional edible oils, however, these oils do not tend to accumulate on the body, even when consumed in high quantities, becoming a great resource in the fight against obesity. Sunflower oil is among the most consumed edible oils in the world, and also, is naturally one of the healthiest. This study s goal was to evalu- ate the influence of ultrasound on the enzymatic hydrolysis of sunflower oil with the enzyme phospolipase A1 (Lecitase Ultra) and use mathematical modeling to obtain simulations of production processes of a diacylglycerol rich oil. Ultrasound generates emulsions of water and oil, thus enhancing the system s interfacial area, and producing higher reaction rates compared to conventional enzymatic processes. Sunflower oil partial hydrolysis was carried in a solvent free environment, in 4 hour periods, with temperatures varying from 30 to 50 °C, enzyme/substrate fractions between 0,6 and 4,0% (m/m) and a fixed water/oil fraction of 5% (m/m). with this goal in mind, experimental designs were developed. Results showed optimal reaction conditions on 40 °C and 1,7% (m/m). Subsequently, fixing the optimal parameters, water/oil fraction was evaluated between 2 and 10% (m/m) in a 12 hour period in order to obtain reaction kinetics. Applying a mathematical model to the kinetic curves it was possible to demonstrate that, for the tested fractions, the 2% water/oil ratio is the most favorable to the production of diacylglycerol, and the maximum quantity of this product is obtained at 27 minutes of reaction time.Óleos e gorduras são alimentos essenciais à dieta humana, porém, o impacto negativo cau- sado por seu consumo excessivo é motivo de grande preocupação no cenário mundial. Óleos ricos em diacilglicerol possuem características organolépticas muito semelhantes àquelas presentes nos óleos comestíveis convencionais, porém, esses óleos não tendem a se acu- mular no organismo, mesmo se consumidos em altas quantidades, tornando-os um grande recurso no combate à obesidade. O óleo de girassol está entre os óleos comestíveis mais consumidos no mundo e também é, naturalmente, um dos mais saudáveis. Esse trabalho teve como objetivo avaliar a influência do ultrassom na hidrólise enzimática do óleo de girassol com a enzima phospolipase A1 (Lecitase Ultra) e utilizar modelagem matemática para obter simulações de processos de produção de um óleo rico em diacilglicerol. O ultrassom gera emulsões de água em óleo, aumentando assim a área interfacial do sistema e produzindo maiores taxas de reação em relação aos processos enzimáticos convencionais. A hidrólise parcial do óleo de girassol foi realizada em meio livre de solventes, em períodos de 4 h, em temperaturas variando de 30 a 50 °C, frações enzima/substrato entre 0,6 e 4,0% (m/m) e fração água/óleo fixa em 5% (m/m). Para isso, foram desenvolvidos dois planejamentos ex- perimentais fatoriais e um delineamento central composto rotacional. Resultados mostraram condições ótimas reacionais em 40 °C e 1,7% (m/m). Posteriormente, com os parâmetros óti- mos fixos, a fração água/óleo foi avaliada no intervalo de 2 a 10% (m/m) em um período de 12 h para a obtenção de cinéticas reacionais. Através de modelagem matemática aplicada às cinéticas reacionais, demonstrou-se que, entre as frações testadas, a razão água/óleo de 2% é a mais favorável para a produção de diacilglicerol, e a quantidade máxima deste produto é obtida aos 27 minutos de reação.Made available in DSpace on 2017-07-10T18:08:06Z (GMT). No. of bitstreams: 1 Eduardo Raizer.pdf: 2599580 bytes, checksum: ba373a374904690cef9ddb1e35744381 (MD5) Previous issue date: 2015-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Estadual do Oeste do ParanaPrograma de Pós-Graduação Stricto Sensu em Engenharia QuímicaUNIOESTEBRDesenvolvimento de ProcessosÓleo de girassolHidróliseHidrólise enzimáticaLecitase UltraSunflower oilHidrolysisEnzymatic hydrolysisLecitase UltraCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAUso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassolUse of ultrasound in diacylglycerol synthesis via enzymatic hydrolysis of sunflower oilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALEduardo Raizer.pdfapplication/pdf2599580http://tede.unioeste.br:8080/tede/bitstream/tede/1861/1/Eduardo+Raizer.pdfba373a374904690cef9ddb1e35744381MD51tede/18612017-07-10 15:08:06.764oai:tede.unioeste.br:tede/1861Biblioteca Digital de Teses e Dissertaçõeshttp://tede.unioeste.br/PUBhttp://tede.unioeste.br/oai/requestbiblioteca.repositorio@unioeste.bropendoar:2017-07-10T18:08:06Biblioteca Digital de Teses e Dissertações do UNIOESTE - Universidade Estadual do Oeste do Paraná (UNIOESTE)false |
dc.title.por.fl_str_mv |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
dc.title.alternative.eng.fl_str_mv |
Use of ultrasound in diacylglycerol synthesis via enzymatic hydrolysis of sunflower oil |
title |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
spellingShingle |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol Raizer, Eduardo Óleo de girassol Hidrólise Hidrólise enzimática Lecitase Ultra Sunflower oil Hidrolysis Enzymatic hydrolysis Lecitase Ultra CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
title_full |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
title_fullStr |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
title_full_unstemmed |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
title_sort |
Uso de ultrassom na síntese de diacilglicerol via hidrólise enzimática de óleo de girassol |
author |
Raizer, Eduardo |
author_facet |
Raizer, Eduardo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cardozo Filho, Lúcio |
dc.contributor.advisor1ID.fl_str_mv |
CPF:10785177892 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2710474728753403 |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Edson Antônio Alves da |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:64656675920 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9304493875700070 |
dc.contributor.referee1.fl_str_mv |
Teleken, Joel Gustavo |
dc.contributor.referee1ID.fl_str_mv |
CPF:04253719996 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6288735286919040 |
dc.contributor.referee2.fl_str_mv |
Borba, Carlos Eduardo |
dc.contributor.referee2ID.fl_str_mv |
CPF:03533333957 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0750048720229101 |
dc.contributor.authorID.fl_str_mv |
CPF:05374424998 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0789820025444227 |
dc.contributor.author.fl_str_mv |
Raizer, Eduardo |
contributor_str_mv |
Cardozo Filho, Lúcio Silva, Edson Antônio Alves da Teleken, Joel Gustavo Borba, Carlos Eduardo |
dc.subject.por.fl_str_mv |
Óleo de girassol Hidrólise Hidrólise enzimática Lecitase Ultra |
topic |
Óleo de girassol Hidrólise Hidrólise enzimática Lecitase Ultra Sunflower oil Hidrolysis Enzymatic hydrolysis Lecitase Ultra CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Sunflower oil Hidrolysis Enzymatic hydrolysis Lecitase Ultra |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
Oils and fats are essential foods to the human diet, nevertheless, the negative impact caused by its overconsumption is of great concern worldwide. Diacylglycerol rich oils have orga- noleptic features very similar to those found in conventional edible oils, however, these oils do not tend to accumulate on the body, even when consumed in high quantities, becoming a great resource in the fight against obesity. Sunflower oil is among the most consumed edible oils in the world, and also, is naturally one of the healthiest. This study s goal was to evalu- ate the influence of ultrasound on the enzymatic hydrolysis of sunflower oil with the enzyme phospolipase A1 (Lecitase Ultra) and use mathematical modeling to obtain simulations of production processes of a diacylglycerol rich oil. Ultrasound generates emulsions of water and oil, thus enhancing the system s interfacial area, and producing higher reaction rates compared to conventional enzymatic processes. Sunflower oil partial hydrolysis was carried in a solvent free environment, in 4 hour periods, with temperatures varying from 30 to 50 °C, enzyme/substrate fractions between 0,6 and 4,0% (m/m) and a fixed water/oil fraction of 5% (m/m). with this goal in mind, experimental designs were developed. Results showed optimal reaction conditions on 40 °C and 1,7% (m/m). Subsequently, fixing the optimal parameters, water/oil fraction was evaluated between 2 and 10% (m/m) in a 12 hour period in order to obtain reaction kinetics. Applying a mathematical model to the kinetic curves it was possible to demonstrate that, for the tested fractions, the 2% water/oil ratio is the most favorable to the production of diacylglycerol, and the maximum quantity of this product is obtained at 27 minutes of reaction time. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-24 |
dc.date.accessioned.fl_str_mv |
2017-07-10T18:08:06Z |
dc.date.available.fl_str_mv |
2017-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RAIZER, Eduardo. Use of ultrasound in diacylglycerol synthesis via enzymatic hydrolysis of sunflower oil. 2015. 69 f. Dissertação (Mestrado em Desenvolvimento de Processos) - Universidade Estadual do Oeste do Parana, Toledo, 2015. |
dc.identifier.uri.fl_str_mv |
http://tede.unioeste.br:8080/tede/handle/tede/1861 |
identifier_str_mv |
RAIZER, Eduardo. Use of ultrasound in diacylglycerol synthesis via enzymatic hydrolysis of sunflower oil. 2015. 69 f. Dissertação (Mestrado em Desenvolvimento de Processos) - Universidade Estadual do Oeste do Parana, Toledo, 2015. |
url |
http://tede.unioeste.br:8080/tede/handle/tede/1861 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação Stricto Sensu em Engenharia Química |
dc.publisher.initials.fl_str_mv |
UNIOESTE |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Desenvolvimento de Processos |
publisher.none.fl_str_mv |
Universidade Estadual do Oeste do Parana |
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Biblioteca Digital de Teses e Dissertações do UNIOESTE |
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