CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION

Detalhes bibliográficos
Autor(a) principal: Grinet, Marco Antonio Vieira Macedo
Data de Publicação: 2017
Outros Autores: Pedroso, Thiago Moreira, Marciano, Fernanda Roberta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista UniVap (online)
Texto Completo: https://revista.univap.br/index.php/revistaunivap/article/view/1679
Resumo: Carbohydrates are vast constituents of cell surfaces and in many systems where cell adhesion plays a critical role, carbohydrate binding proteins have been shown to bind to cell surface carbohydrates and participate in cell-cell interactions. Jurkat cells are suspension cells that grow in clumps and have 20.7 (± 2.2) hours of population doubling time (PDT). In this experiment, Jurkat cells are studied to compare the effects of wheat germ agglutinin (WGA) lectin, and Maackia amurensis (MAA) lectin, for clumping and spheroid formation studies, as well as carbohydrate analog solutions in ethanol (C2H6O) Ac4ManNAc, and Ac5ManNTGc for concentration effect studies.
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spelling CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATIONCell AdhesionJurkat cellsSpheroidsCarbohydratesLectinCarbohydrates are vast constituents of cell surfaces and in many systems where cell adhesion plays a critical role, carbohydrate binding proteins have been shown to bind to cell surface carbohydrates and participate in cell-cell interactions. Jurkat cells are suspension cells that grow in clumps and have 20.7 (± 2.2) hours of population doubling time (PDT). In this experiment, Jurkat cells are studied to compare the effects of wheat germ agglutinin (WGA) lectin, and Maackia amurensis (MAA) lectin, for clumping and spheroid formation studies, as well as carbohydrate analog solutions in ethanol (C2H6O) Ac4ManNAc, and Ac5ManNTGc for concentration effect studies.IBICT2017-04-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.univap.br/index.php/revistaunivap/article/view/167910.18066/revistaunivap.v22i40.1679Revista Univap; Vol. 22 No. 40 (2016): Revista Univap online Edição Especial XX Encontro de Iniciação Científica, XVI Encontro de Pós-Graduação, X INIC Jr e VI INID da Universidade do Vale do Paraíba / ISSN 2237-1753; 834Revista Univap; v. 22 n. 40 (2016): Revista Univap online Edição Especial XX Encontro de Iniciação Científica, XVI Encontro de Pós-Graduação, X INIC Jr e VI INID da Universidade do Vale do Paraíba / ISSN 2237-1753; 8342237-17531517-327510.18066/revistaunivap.v22i40reponame:Revista UniVap (online)instname:Universidade do Vale do Paraíba (Univap)instacron:UNIVAPenghttps://revista.univap.br/index.php/revistaunivap/article/view/1679/1290Copyright (c) 2017 Revista Univapinfo:eu-repo/semantics/openAccessGrinet, Marco Antonio Vieira MacedoPedroso, Thiago MoreiraMarciano, Fernanda Roberta2020-01-08T15:48:39Zoai:ojs.biblioteca.univap.br:article/1679Revistahttps://revista.univap.br/index.php/revistaunivapPRIhttps://revista.univap.br/index.php/revistaunivap/oairevista@univap.br2237-17532237-1753opendoar:2020-01-08T15:48:39Revista UniVap (online) - Universidade do Vale do Paraíba (Univap)false
dc.title.none.fl_str_mv CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
title CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
spellingShingle CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
Grinet, Marco Antonio Vieira Macedo
Cell Adhesion
Jurkat cells
Spheroids
Carbohydrates
Lectin
title_short CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
title_full CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
title_fullStr CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
title_full_unstemmed CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
title_sort CARBOHYDRATE-BASED CELL ADHESION: ANALYSIS OF SPHEROID FORMATION
author Grinet, Marco Antonio Vieira Macedo
author_facet Grinet, Marco Antonio Vieira Macedo
Pedroso, Thiago Moreira
Marciano, Fernanda Roberta
author_role author
author2 Pedroso, Thiago Moreira
Marciano, Fernanda Roberta
author2_role author
author
dc.contributor.author.fl_str_mv Grinet, Marco Antonio Vieira Macedo
Pedroso, Thiago Moreira
Marciano, Fernanda Roberta
dc.subject.por.fl_str_mv Cell Adhesion
Jurkat cells
Spheroids
Carbohydrates
Lectin
topic Cell Adhesion
Jurkat cells
Spheroids
Carbohydrates
Lectin
description Carbohydrates are vast constituents of cell surfaces and in many systems where cell adhesion plays a critical role, carbohydrate binding proteins have been shown to bind to cell surface carbohydrates and participate in cell-cell interactions. Jurkat cells are suspension cells that grow in clumps and have 20.7 (± 2.2) hours of population doubling time (PDT). In this experiment, Jurkat cells are studied to compare the effects of wheat germ agglutinin (WGA) lectin, and Maackia amurensis (MAA) lectin, for clumping and spheroid formation studies, as well as carbohydrate analog solutions in ethanol (C2H6O) Ac4ManNAc, and Ac5ManNTGc for concentration effect studies.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revista.univap.br/index.php/revistaunivap/article/view/1679
10.18066/revistaunivap.v22i40.1679
url https://revista.univap.br/index.php/revistaunivap/article/view/1679
identifier_str_mv 10.18066/revistaunivap.v22i40.1679
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revista.univap.br/index.php/revistaunivap/article/view/1679/1290
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista Univap
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista Univap
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IBICT
publisher.none.fl_str_mv IBICT
dc.source.none.fl_str_mv Revista Univap; Vol. 22 No. 40 (2016): Revista Univap online Edição Especial XX Encontro de Iniciação Científica, XVI Encontro de Pós-Graduação, X INIC Jr e VI INID da Universidade do Vale do Paraíba / ISSN 2237-1753; 834
Revista Univap; v. 22 n. 40 (2016): Revista Univap online Edição Especial XX Encontro de Iniciação Científica, XVI Encontro de Pós-Graduação, X INIC Jr e VI INID da Universidade do Vale do Paraíba / ISSN 2237-1753; 834
2237-1753
1517-3275
10.18066/revistaunivap.v22i40
reponame:Revista UniVap (online)
instname:Universidade do Vale do Paraíba (Univap)
instacron:UNIVAP
instname_str Universidade do Vale do Paraíba (Univap)
instacron_str UNIVAP
institution UNIVAP
reponame_str Revista UniVap (online)
collection Revista UniVap (online)
repository.name.fl_str_mv Revista UniVap (online) - Universidade do Vale do Paraíba (Univap)
repository.mail.fl_str_mv revista@univap.br
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