Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim

Detalhes bibliográficos
Autor(a) principal: Homczinski, Isabel
Data de Publicação: 2023
Outros Autores: dos Santos, Jonathan Matheus, Sanson, Daniela, Garrett, Alexandre Techy de Almeida, Peres, Fabiana Schmidt Bandeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Evidência - Ciência e Biotecnologia - Interdisciplinar
Texto Completo: https://periodicos.unoesc.edu.br/evidencia/article/view/32526
Resumo: Prunus campanulata is a species largely adopted for landscape composition in Brazil with limited seedling production by seed dormancy, lacking studies about in vitro propagation. This study aims to evaluate different P. campanulata seed pretreatments on germination, and the effect of GA3 on dormancy break and seedlings biometry. Seeds were processed in three treatments: complete seeds, seeds without endocarp, and isolated zygotic embryos incubated in a WPM medium containing different GA3 concentrations (0.0, 2.0, and 4.0 mg L-1). After 30 days of in vitro incubation were evaluated the germination percentage, mean germination time, percentage of normal seedlings, percentage of seedlings with leaves, and survival percentage were. Seedlings greater than 3.0 cm were selected for ex vitro adaptation, evaluating after 32 days the biometric variables: total length, aerial part length, root length, and the number of leaves. Complete seeds do not germinate, suggesting dormancy associated with the endocarp, whereas seeds without endocarp and isolated zygotic embryos showed the same germination rate, with no effect on GA3. The GA3 promoted greater seedling growth at concentrations between 1.89 mg L-1 for the total length of the isolated zygotic embryo and 2.24 mg L-1 for the length of the aerial part of seeds without endocarp. In conclusion, seed processing, i.e., removing the endocarp or isolating the zygotic embryo can overcome dormancy, improving germination and seedling production of P. campanulata in vitro.
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spelling Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata MaximQuebra de dormência induzida por ácido giberélico e germinação in vitro de sementes e embriões zigóticos de Prunus campanulata MaximGA3Japanese cherry treeIn vitro embryosBiometryGA3Cerejeira japonesaEmbriões in vitroBiometriaPrunus campanulata is a species largely adopted for landscape composition in Brazil with limited seedling production by seed dormancy, lacking studies about in vitro propagation. This study aims to evaluate different P. campanulata seed pretreatments on germination, and the effect of GA3 on dormancy break and seedlings biometry. Seeds were processed in three treatments: complete seeds, seeds without endocarp, and isolated zygotic embryos incubated in a WPM medium containing different GA3 concentrations (0.0, 2.0, and 4.0 mg L-1). After 30 days of in vitro incubation were evaluated the germination percentage, mean germination time, percentage of normal seedlings, percentage of seedlings with leaves, and survival percentage were. Seedlings greater than 3.0 cm were selected for ex vitro adaptation, evaluating after 32 days the biometric variables: total length, aerial part length, root length, and the number of leaves. Complete seeds do not germinate, suggesting dormancy associated with the endocarp, whereas seeds without endocarp and isolated zygotic embryos showed the same germination rate, with no effect on GA3. The GA3 promoted greater seedling growth at concentrations between 1.89 mg L-1 for the total length of the isolated zygotic embryo and 2.24 mg L-1 for the length of the aerial part of seeds without endocarp. In conclusion, seed processing, i.e., removing the endocarp or isolating the zygotic embryo can overcome dormancy, improving germination and seedling production of P. campanulata in vitro.Prunus campanulata é uma espécie amplamente utilizada para composição de paisagens no Brasil com limitada produção de mudas por dormência de sementes, carecendo de estudos sobre propagação in vitro. Este trabalho tem como objetivo avaliar diferentes pré-tratamentos de sementes de P. campanulata na germinação e o efeito do GA3 na quebra de dormência e na biometria de plântulas. As sementes foram processadas em três tratamentos: sementes completas, sementes sem endocarpo e embriões zigóticos isolados incubados em meio WPM contendo diferentes concentrações de GA3 (0,0; 2,0 e 4,0 mg L-1). Após 30 dias de incubação in vitro foram avaliados o percentual de germinação, o tempo médio de germinação, o percentual de plântulas normais, o percentual de plântulas com folhas e o percentual de sobrevivência. Mudas maiores que 3,0 cm foram selecionadas para adaptação ex vitro, avaliando-se após 32 dias as variáveis biométricas: comprimento total, comprimento da parte aérea, comprimento da raiz e número de folhas. Sementes completas não germinam, sugerindo dormência associada ao endocarpo, enquanto sementes sem endocarpo e embriões zigóticos isolados apresentaram a mesma taxa de germinação, sem efeito sobre GA3. O GA3 promoveu maior crescimento de plântulas em concentrações entre 1,89 mg L-1 para o comprimento total do embrião zigótico isolado e 2,24 mg L-1 para o comprimento da parte aérea das sementes sem endocarpo. Em conclusão, o processamento de sementes, ou seja, a remoção do endocarpo ou o isolamento do embrião zigótico pode superar a dormência, melhorando a germinação e a produção de plântulas de P. campanulata in vitro.Universidade do Oeste de Santa Catarina2023-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://periodicos.unoesc.edu.br/evidencia/article/view/3252610.18593/evid.32526Evidence; Vol. 23 No. 1 (2023): Evidência; 9-22Evidência; v. 23 n. 1 (2023): Evidência; 9-222236-60591519-5287reponame:Evidência - Ciência e Biotecnologia - Interdisciplinarinstname:Universidade do Oeste de Santa Catarina (UNOESC)instacron:UNOESCenghttps://periodicos.unoesc.edu.br/evidencia/article/view/32526/18424https://periodicos.unoesc.edu.br/evidencia/article/view/32526/18488Copyright (c) 2023 Evidênciahttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessHomczinski, Isabeldos Santos, Jonathan MatheusSanson, DanielaGarrett, Alexandre Techy de AlmeidaPeres, Fabiana Schmidt Bandeira2023-11-27T14:12:10Zoai:ojs.periodicos.unoesc.edu.br:article/32526Revistahttps://periodicos.unoesc.edu.br/evidencia/PUBhttps://periodicos.unoesc.edu.br/evidencia/oaieditora@unoesc.edu.br||evidencia@unoesc.edu.br||jane.gelinski@unoesc.edu.br|| debora.pereira@unoesc.edu.br2236-60591519-5287opendoar:2023-11-27T14:12:10Evidência - Ciência e Biotecnologia - Interdisciplinar - Universidade do Oeste de Santa Catarina (UNOESC)false
dc.title.none.fl_str_mv Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
Quebra de dormência induzida por ácido giberélico e germinação in vitro de sementes e embriões zigóticos de Prunus campanulata Maxim
title Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
spellingShingle Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
Homczinski, Isabel
GA3
Japanese cherry tree
In vitro embryos
Biometry
GA3
Cerejeira japonesa
Embriões in vitro
Biometria
title_short Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
title_full Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
title_fullStr Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
title_full_unstemmed Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
title_sort Dormancy break induced by gibberellic acid and in vitro germination of seeds and zygotic embryos of Prunus campanulata Maxim
author Homczinski, Isabel
author_facet Homczinski, Isabel
dos Santos, Jonathan Matheus
Sanson, Daniela
Garrett, Alexandre Techy de Almeida
Peres, Fabiana Schmidt Bandeira
author_role author
author2 dos Santos, Jonathan Matheus
Sanson, Daniela
Garrett, Alexandre Techy de Almeida
Peres, Fabiana Schmidt Bandeira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Homczinski, Isabel
dos Santos, Jonathan Matheus
Sanson, Daniela
Garrett, Alexandre Techy de Almeida
Peres, Fabiana Schmidt Bandeira
dc.subject.por.fl_str_mv GA3
Japanese cherry tree
In vitro embryos
Biometry
GA3
Cerejeira japonesa
Embriões in vitro
Biometria
topic GA3
Japanese cherry tree
In vitro embryos
Biometry
GA3
Cerejeira japonesa
Embriões in vitro
Biometria
description Prunus campanulata is a species largely adopted for landscape composition in Brazil with limited seedling production by seed dormancy, lacking studies about in vitro propagation. This study aims to evaluate different P. campanulata seed pretreatments on germination, and the effect of GA3 on dormancy break and seedlings biometry. Seeds were processed in three treatments: complete seeds, seeds without endocarp, and isolated zygotic embryos incubated in a WPM medium containing different GA3 concentrations (0.0, 2.0, and 4.0 mg L-1). After 30 days of in vitro incubation were evaluated the germination percentage, mean germination time, percentage of normal seedlings, percentage of seedlings with leaves, and survival percentage were. Seedlings greater than 3.0 cm were selected for ex vitro adaptation, evaluating after 32 days the biometric variables: total length, aerial part length, root length, and the number of leaves. Complete seeds do not germinate, suggesting dormancy associated with the endocarp, whereas seeds without endocarp and isolated zygotic embryos showed the same germination rate, with no effect on GA3. The GA3 promoted greater seedling growth at concentrations between 1.89 mg L-1 for the total length of the isolated zygotic embryo and 2.24 mg L-1 for the length of the aerial part of seeds without endocarp. In conclusion, seed processing, i.e., removing the endocarp or isolating the zygotic embryo can overcome dormancy, improving germination and seedling production of P. campanulata in vitro.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.unoesc.edu.br/evidencia/article/view/32526
10.18593/evid.32526
url https://periodicos.unoesc.edu.br/evidencia/article/view/32526
identifier_str_mv 10.18593/evid.32526
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.unoesc.edu.br/evidencia/article/view/32526/18424
https://periodicos.unoesc.edu.br/evidencia/article/view/32526/18488
dc.rights.driver.fl_str_mv Copyright (c) 2023 Evidência
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Evidência
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade do Oeste de Santa Catarina
publisher.none.fl_str_mv Universidade do Oeste de Santa Catarina
dc.source.none.fl_str_mv Evidence; Vol. 23 No. 1 (2023): Evidência; 9-22
Evidência; v. 23 n. 1 (2023): Evidência; 9-22
2236-6059
1519-5287
reponame:Evidência - Ciência e Biotecnologia - Interdisciplinar
instname:Universidade do Oeste de Santa Catarina (UNOESC)
instacron:UNOESC
instname_str Universidade do Oeste de Santa Catarina (UNOESC)
instacron_str UNOESC
institution UNOESC
reponame_str Evidência - Ciência e Biotecnologia - Interdisciplinar
collection Evidência - Ciência e Biotecnologia - Interdisciplinar
repository.name.fl_str_mv Evidência - Ciência e Biotecnologia - Interdisciplinar - Universidade do Oeste de Santa Catarina (UNOESC)
repository.mail.fl_str_mv editora@unoesc.edu.br||evidencia@unoesc.edu.br||jane.gelinski@unoesc.edu.br|| debora.pereira@unoesc.edu.br
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