The Role of Lipotoxicity in Smoke Cardiomyopathy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0113739 http://hdl.handle.net/11449/117417 |
Resumo: | Background/Aims: Experimental and clinical studies have shown the direct toxic effects of cigarette smoke (CS) on the myocardium, independent of vascular effects. However, the underlying mechanisms are not well known.Methods: Wistar rats were allocated to control (C) and cigarette smoke (CS) groups. CS rats were exposed to cigarette smoke for 2 months.Results: After that morphometric, functional and biochemical parameters were measured. The echocardiographic study showed enlargement of the left atria, increase in the left ventricular systolic volume and reduced systolic function. Within the cardiac metabolism, exposure to CS decreased beta hydroxy acyl coenzyme A dehydrogenases and citrate synthases and increased lactate dehydrogenases. Peroxisome proliferator-activated receptor alpha (PPAR alpha) and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) were expressed similarly in both groups. CS increased serum lipids and myocardial triacylglycerols (TGs). These data suggest that impairment in fatty acid oxidation and the accumulation of cardiac lipids characterize lipotoxicity. CS group exhibited increased oxidative stress and decreased antioxidant defense. Finally, the myocyte cross-sectional area and active Caspase 3 were increased in the CS group.Conclusion: The cardiac remodeling that was observed in the CS exposure model may be explained by abnormalities in energy metabolism, including lipotoxicity and oxidative stress. |
id |
UNSP_0027239795f864e2b38180024208f132 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/117417 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
The Role of Lipotoxicity in Smoke CardiomyopathyBackground/Aims: Experimental and clinical studies have shown the direct toxic effects of cigarette smoke (CS) on the myocardium, independent of vascular effects. However, the underlying mechanisms are not well known.Methods: Wistar rats were allocated to control (C) and cigarette smoke (CS) groups. CS rats were exposed to cigarette smoke for 2 months.Results: After that morphometric, functional and biochemical parameters were measured. The echocardiographic study showed enlargement of the left atria, increase in the left ventricular systolic volume and reduced systolic function. Within the cardiac metabolism, exposure to CS decreased beta hydroxy acyl coenzyme A dehydrogenases and citrate synthases and increased lactate dehydrogenases. Peroxisome proliferator-activated receptor alpha (PPAR alpha) and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) were expressed similarly in both groups. CS increased serum lipids and myocardial triacylglycerols (TGs). These data suggest that impairment in fatty acid oxidation and the accumulation of cardiac lipids characterize lipotoxicity. CS group exhibited increased oxidative stress and decreased antioxidant defense. Finally, the myocyte cross-sectional area and active Caspase 3 were increased in the CS group.Conclusion: The cardiac remodeling that was observed in the CS exposure model may be explained by abnormalities in energy metabolism, including lipotoxicity and oxidative stress.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Botucatu Medical School, UNESP - Univ Estadual Paulista, Botucatu, Sao Paulo, BrasilUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Chem & Biochem, Botucatu, SP, BrazilCornell Univ, Weill Med Coll, Ctr Vasc Biol, Dept Pathol & Lab Med, New York, NY 10021 USAUNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUNESP Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Chem & Biochem, Botucatu, SP, BrazilPublic Library ScienceUniversidade Estadual Paulista (Unesp)Cornell UnivSantos, Priscila P. [UNESP]Oliveira, Fernando [UNESP]Ferreira, Vanessa C. M. P. [UNESP]Polegato, Bertha Furlan [UNESP]Roscani, Meliza Goi [UNESP]Fernandes, Ana Angelica [UNESP]Modesto, Pamela [UNESP]Rafacho, Bruna P. M. [UNESP]Zanati, Silmeia G. [UNESP]Di Lorenzo, AnnaritaMatsubara, Luiz Shiguero [UNESP]Paiva, Sergio Alberto Rupp de [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Minicucci, Marcos Ferreira [UNESP]Gaiolla, Paula Schmidt Azevedo [UNESP]2015-03-18T15:56:05Z2015-03-18T15:56:05Z2014-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://dx.doi.org/10.1371/journal.pone.0113739Plos One. San Francisco: Public Library Science, v. 9, n. 12, 13 p., 2014.1932-6203http://hdl.handle.net/11449/11741710.1371/journal.pone.0113739WOS:000345869700045WOS000345869700045.pdf63098351379987665016839015394547121314080140264774387040344716730000-0002-5843-6232Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2024-08-14T17:22:25Zoai:repositorio.unesp.br:11449/117417Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
title |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
spellingShingle |
The Role of Lipotoxicity in Smoke Cardiomyopathy Santos, Priscila P. [UNESP] |
title_short |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
title_full |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
title_fullStr |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
title_full_unstemmed |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
title_sort |
The Role of Lipotoxicity in Smoke Cardiomyopathy |
author |
Santos, Priscila P. [UNESP] |
author_facet |
Santos, Priscila P. [UNESP] Oliveira, Fernando [UNESP] Ferreira, Vanessa C. M. P. [UNESP] Polegato, Bertha Furlan [UNESP] Roscani, Meliza Goi [UNESP] Fernandes, Ana Angelica [UNESP] Modesto, Pamela [UNESP] Rafacho, Bruna P. M. [UNESP] Zanati, Silmeia G. [UNESP] Di Lorenzo, Annarita Matsubara, Luiz Shiguero [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] Gaiolla, Paula Schmidt Azevedo [UNESP] |
author_role |
author |
author2 |
Oliveira, Fernando [UNESP] Ferreira, Vanessa C. M. P. [UNESP] Polegato, Bertha Furlan [UNESP] Roscani, Meliza Goi [UNESP] Fernandes, Ana Angelica [UNESP] Modesto, Pamela [UNESP] Rafacho, Bruna P. M. [UNESP] Zanati, Silmeia G. [UNESP] Di Lorenzo, Annarita Matsubara, Luiz Shiguero [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] Gaiolla, Paula Schmidt Azevedo [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Cornell Univ |
dc.contributor.author.fl_str_mv |
Santos, Priscila P. [UNESP] Oliveira, Fernando [UNESP] Ferreira, Vanessa C. M. P. [UNESP] Polegato, Bertha Furlan [UNESP] Roscani, Meliza Goi [UNESP] Fernandes, Ana Angelica [UNESP] Modesto, Pamela [UNESP] Rafacho, Bruna P. M. [UNESP] Zanati, Silmeia G. [UNESP] Di Lorenzo, Annarita Matsubara, Luiz Shiguero [UNESP] Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] Gaiolla, Paula Schmidt Azevedo [UNESP] |
description |
Background/Aims: Experimental and clinical studies have shown the direct toxic effects of cigarette smoke (CS) on the myocardium, independent of vascular effects. However, the underlying mechanisms are not well known.Methods: Wistar rats were allocated to control (C) and cigarette smoke (CS) groups. CS rats were exposed to cigarette smoke for 2 months.Results: After that morphometric, functional and biochemical parameters were measured. The echocardiographic study showed enlargement of the left atria, increase in the left ventricular systolic volume and reduced systolic function. Within the cardiac metabolism, exposure to CS decreased beta hydroxy acyl coenzyme A dehydrogenases and citrate synthases and increased lactate dehydrogenases. Peroxisome proliferator-activated receptor alpha (PPAR alpha) and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) were expressed similarly in both groups. CS increased serum lipids and myocardial triacylglycerols (TGs). These data suggest that impairment in fatty acid oxidation and the accumulation of cardiac lipids characterize lipotoxicity. CS group exhibited increased oxidative stress and decreased antioxidant defense. Finally, the myocyte cross-sectional area and active Caspase 3 were increased in the CS group.Conclusion: The cardiac remodeling that was observed in the CS exposure model may be explained by abnormalities in energy metabolism, including lipotoxicity and oxidative stress. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-02 2015-03-18T15:56:05Z 2015-03-18T15:56:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0113739 Plos One. San Francisco: Public Library Science, v. 9, n. 12, 13 p., 2014. 1932-6203 http://hdl.handle.net/11449/117417 10.1371/journal.pone.0113739 WOS:000345869700045 WOS000345869700045.pdf 6309835137998766 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
url |
http://dx.doi.org/10.1371/journal.pone.0113739 http://hdl.handle.net/11449/117417 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 12, 13 p., 2014. 1932-6203 10.1371/journal.pone.0113739 WOS:000345869700045 WOS000345869700045.pdf 6309835137998766 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128117419540480 |