Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia

Detalhes bibliográficos
Autor(a) principal: Da Silva, Daniele Caetano [UNESP]
Data de Publicação: 2014
Outros Autores: Trídico, Camila Pereira [UNESP], Serrano, Lenard [UNESP], De Almeida, Eduardo Alves [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.15343/0104-7809.20143801009015
http://hdl.handle.net/11449/227729
Resumo: Currently, with the growing contamination of aquatic ecosystems, many compounds, such as pesticides and hydrocarbons, are improperly released into rivers and lakes. Studies about the exposure of single pollutants causing biochemical variations are abundant in the literature. However, there are few studies focused on the biological effects of complex mixtures. The aim of this investigation was to evaluate whether mixture diazinon and benzo[a]pyrene can affect the biochemical activities of classic biomarkers such as acetylcholinesterase (AChE), carboxylesterase (CbE), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in Nile tilapia and compare the effects on enzymatic systems of the exposure to a mixture of compounds and the effects observed when they are exposed separately. We measured the activity of enzymes in gills and liver of Nile Tilapia (Oreochromis niloticus) after 2 and 7 days of exposure to Diazinon (0.5 mg/L) and benzo[a]pyrene (1.0 mg/L) individually and in mixture. The results showed that in the mixture group after 7 days of exposure, the benzo[a]pyrene increased the inhibitory action of Diazinon in GST enzyme activity, in liver tissue. This indicates that the toxicity of the interactions between pesticide and polycyclic aromatic hydrocarbon may show synergistic effect. These results suggest the importance of studies with mixture of compounds, because these data will help to understand the results obtained in field studies, such as those from environmental monitoring.
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spelling Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapiaBenzo[a]pyreneBiomarkersCichlidsDiazinonPharmacologicalCurrently, with the growing contamination of aquatic ecosystems, many compounds, such as pesticides and hydrocarbons, are improperly released into rivers and lakes. Studies about the exposure of single pollutants causing biochemical variations are abundant in the literature. However, there are few studies focused on the biological effects of complex mixtures. The aim of this investigation was to evaluate whether mixture diazinon and benzo[a]pyrene can affect the biochemical activities of classic biomarkers such as acetylcholinesterase (AChE), carboxylesterase (CbE), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in Nile tilapia and compare the effects on enzymatic systems of the exposure to a mixture of compounds and the effects observed when they are exposed separately. We measured the activity of enzymes in gills and liver of Nile Tilapia (Oreochromis niloticus) after 2 and 7 days of exposure to Diazinon (0.5 mg/L) and benzo[a]pyrene (1.0 mg/L) individually and in mixture. The results showed that in the mixture group after 7 days of exposure, the benzo[a]pyrene increased the inhibitory action of Diazinon in GST enzyme activity, in liver tissue. This indicates that the toxicity of the interactions between pesticide and polycyclic aromatic hydrocarbon may show synergistic effect. These results suggest the importance of studies with mixture of compounds, because these data will help to understand the results obtained in field studies, such as those from environmental monitoring.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, São José do Rio Preto-SPUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, São José do Rio Preto-SPUniversidade Estadual Paulista (UNESP)Da Silva, Daniele Caetano [UNESP]Trídico, Camila Pereira [UNESP]Serrano, Lenard [UNESP]De Almeida, Eduardo Alves [UNESP]2022-04-29T07:14:51Z2022-04-29T07:14:51Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9-15http://dx.doi.org/10.15343/0104-7809.20143801009015Mundo da Saude, v. 38, n. 1, p. 9-15, 2014.1980-39900104-7809http://hdl.handle.net/11449/22772910.15343/0104-7809.201438010090152-s2.0-84899826915Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMundo da Saudeinfo:eu-repo/semantics/openAccess2022-04-29T07:14:51Zoai:repositorio.unesp.br:11449/227729Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:48:47.049395Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
title Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
spellingShingle Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
Da Silva, Daniele Caetano [UNESP]
Benzo[a]pyrene
Biomarkers
Cichlids
Diazinon
Pharmacological
title_short Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
title_full Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
title_fullStr Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
title_full_unstemmed Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
title_sort Effect of mixture of diazinon and benzo[a]pyrene in Glutathione S-transferase of Nile tilapia
author Da Silva, Daniele Caetano [UNESP]
author_facet Da Silva, Daniele Caetano [UNESP]
Trídico, Camila Pereira [UNESP]
Serrano, Lenard [UNESP]
De Almeida, Eduardo Alves [UNESP]
author_role author
author2 Trídico, Camila Pereira [UNESP]
Serrano, Lenard [UNESP]
De Almeida, Eduardo Alves [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Da Silva, Daniele Caetano [UNESP]
Trídico, Camila Pereira [UNESP]
Serrano, Lenard [UNESP]
De Almeida, Eduardo Alves [UNESP]
dc.subject.por.fl_str_mv Benzo[a]pyrene
Biomarkers
Cichlids
Diazinon
Pharmacological
topic Benzo[a]pyrene
Biomarkers
Cichlids
Diazinon
Pharmacological
description Currently, with the growing contamination of aquatic ecosystems, many compounds, such as pesticides and hydrocarbons, are improperly released into rivers and lakes. Studies about the exposure of single pollutants causing biochemical variations are abundant in the literature. However, there are few studies focused on the biological effects of complex mixtures. The aim of this investigation was to evaluate whether mixture diazinon and benzo[a]pyrene can affect the biochemical activities of classic biomarkers such as acetylcholinesterase (AChE), carboxylesterase (CbE), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in Nile tilapia and compare the effects on enzymatic systems of the exposure to a mixture of compounds and the effects observed when they are exposed separately. We measured the activity of enzymes in gills and liver of Nile Tilapia (Oreochromis niloticus) after 2 and 7 days of exposure to Diazinon (0.5 mg/L) and benzo[a]pyrene (1.0 mg/L) individually and in mixture. The results showed that in the mixture group after 7 days of exposure, the benzo[a]pyrene increased the inhibitory action of Diazinon in GST enzyme activity, in liver tissue. This indicates that the toxicity of the interactions between pesticide and polycyclic aromatic hydrocarbon may show synergistic effect. These results suggest the importance of studies with mixture of compounds, because these data will help to understand the results obtained in field studies, such as those from environmental monitoring.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2022-04-29T07:14:51Z
2022-04-29T07:14:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.15343/0104-7809.20143801009015
Mundo da Saude, v. 38, n. 1, p. 9-15, 2014.
1980-3990
0104-7809
http://hdl.handle.net/11449/227729
10.15343/0104-7809.20143801009015
2-s2.0-84899826915
url http://dx.doi.org/10.15343/0104-7809.20143801009015
http://hdl.handle.net/11449/227729
identifier_str_mv Mundo da Saude, v. 38, n. 1, p. 9-15, 2014.
1980-3990
0104-7809
10.15343/0104-7809.20143801009015
2-s2.0-84899826915
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mundo da Saude
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9-15
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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