Propriedades mecânicas, físico-químicas e biológica de adesivo experimental com incorporação de epigalocatequina-3-galato

• Autor(a) principal: Fonseca, Beatriz Maria da [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/136681; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/28-03-2016/000860767.pdf
• topic: Adesivos dentarios; Dentina; Monomeros; Resinas compostas; Dentin

The aim of this study was to evaluate the effect of EGCG in an experimental adhesive to reduce the hybrid layer degradation, through mechanical, physical-chemical and biological analysis. The first step of this study involved manipulation of the adhesive model with the incorporation three different concentrations of EGCG (0.5%, 1.0% and 1.5%) in relation BisGMA 55% / 45% HEMA, 0.5% camphorquinone and 0.5% DMAEMA. The conversion degree (DC) of adhesive monomers was evaluated by micro-Raman spectroscopy (GC: EGCG 1.5 (1.5%) - 99,11a; EGCG 1.0 (1.0%) - 99,02a; EGCG 0.5 (0.5%) - 96,58ab; Control - 85,47b). The second step involved the mechanical and physicochemical analysis [flexural strength (FS) and elastic modulus (EM); resiliency modulus (RM) and compressive bond strength (CBS), sorption (A) and solubility (S) in water; shrinkage polymerization (SP)]; microtensile bond strength test (μTBS) immediately (24 h) and longitudinal (6 months) and biological analyze [cytotoxicity assay (CY)]. The results were: FS, EM, RM, CBS and SP showed no significant difference between groups; A and S tests showed reduced values only for EGCG 0.5 and 1.0 groups. RA immediately (24) did not differ between groups. After six months there was a reduction of the values only for the control and EGCG 1.5 groups, EGCG groups 0.5 and 1.0 maintained the RA values. CY: EGCG incorporation increased the cell viability in 0.1% and 1.0% dilutions compared to the control group. It was concluded incorporation of EGCG at 0.5, 1.0 and 1.5 concentrations did not affect the degree of conversion of the monomers. Thus, the incorporation of 0.5% (100 μg/ml) and 1.0% (200 μg/ml) EGCG amount can preserve the hybrid layer formed and prolong longevity composite resin restorations in addition to increasing cell viability.