Cheap assays of malate, glycerol and ethanol in fermenting must and food samples

Detalhes bibliográficos
Autor(a) principal: Gattas, EAL
Data de Publicação: 2003
Outros Autores: Tininis, CRCS, Lauce, C.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/7614
Resumo: A preparation, enriched with malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glycerol -3- P dehydrogenase (GPDH) and glycerol kinase (GK), was obtained from dry baker's yeast. This preparation was used to assay glycerol, ethanol and malate measuring the variations in absorbance (NADH formation) at 340 nm. Good degrees of recoveries were obtained when glycerol was added to red wine and fermenting sugar-cane juice and when L-malate was added to commercial apple juice samples. Good results were also obtained when ethanol was assayed in fermented sugar-cane juice and wine samples, using both the partially purified preparation obtained from dry yeast and a purified commercial alcohol dehydrogenase.
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spelling Cheap assays of malate, glycerol and ethanol in fermenting must and food samplesdry baker's yeastglycerolethanolmalate assayA preparation, enriched with malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glycerol -3- P dehydrogenase (GPDH) and glycerol kinase (GK), was obtained from dry baker's yeast. This preparation was used to assay glycerol, ethanol and malate measuring the variations in absorbance (NADH formation) at 340 nm. Good degrees of recoveries were obtained when glycerol was added to red wine and fermenting sugar-cane juice and when L-malate was added to commercial apple juice samples. Good results were also obtained when ethanol was assayed in fermented sugar-cane juice and wine samples, using both the partially purified preparation obtained from dry yeast and a purified commercial alcohol dehydrogenase.UNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, BrazilUNESP, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, BrazilMedimond S R LUniversidade Estadual Paulista (Unesp)Gattas, EALTininis, CRCSLauce, C.2014-05-20T13:24:30Z2014-05-20T13:24:30Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject383-386Euromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine. 40128 Bologna: Medimond S R L, p. 383-386, 2003.http://hdl.handle.net/11449/7614WOS:000184180200070Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicineinfo:eu-repo/semantics/openAccess2024-06-21T12:47:34Zoai:repositorio.unesp.br:11449/7614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-21T12:47:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
title Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
spellingShingle Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
Gattas, EAL
dry baker's yeast
glycerol
ethanol
malate assay
title_short Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
title_full Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
title_fullStr Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
title_full_unstemmed Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
title_sort Cheap assays of malate, glycerol and ethanol in fermenting must and food samples
author Gattas, EAL
author_facet Gattas, EAL
Tininis, CRCS
Lauce, C.
author_role author
author2 Tininis, CRCS
Lauce, C.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gattas, EAL
Tininis, CRCS
Lauce, C.
dc.subject.por.fl_str_mv dry baker's yeast
glycerol
ethanol
malate assay
topic dry baker's yeast
glycerol
ethanol
malate assay
description A preparation, enriched with malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glycerol -3- P dehydrogenase (GPDH) and glycerol kinase (GK), was obtained from dry baker's yeast. This preparation was used to assay glycerol, ethanol and malate measuring the variations in absorbance (NADH formation) at 340 nm. Good degrees of recoveries were obtained when glycerol was added to red wine and fermenting sugar-cane juice and when L-malate was added to commercial apple juice samples. Good results were also obtained when ethanol was assayed in fermented sugar-cane juice and wine samples, using both the partially purified preparation obtained from dry yeast and a purified commercial alcohol dehydrogenase.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
2014-05-20T13:24:30Z
2014-05-20T13:24:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv Euromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine. 40128 Bologna: Medimond S R L, p. 383-386, 2003.
http://hdl.handle.net/11449/7614
WOS:000184180200070
identifier_str_mv Euromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine. 40128 Bologna: Medimond S R L, p. 383-386, 2003.
WOS:000184180200070
url http://hdl.handle.net/11449/7614
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Euromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 383-386
dc.publisher.none.fl_str_mv Medimond S R L
publisher.none.fl_str_mv Medimond S R L
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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