Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/polym14194170 http://hdl.handle.net/11449/248988 |
Resumo: | In this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T. |
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Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defectsbiomaterialbone repairbone substitutefibrin sealantlow-level laser therapyphotobiomodulation therapypolymersscaffoldsIn this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T.Department of Biological Sciences Bauru School of Dentistry FOB/USP University of São PauloStructural and Functional Interactions in Rehabilitation Postgraduate Department University of Marilia (UNIMAR)Teaching and Research Coordination of the Medical School University Center of Adamantina (UNIFAI)Department of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (FOB/USP)Department of Basic and Oral Biology School of Dentistry of Ribeirão Preto University of São Paulo (FORP/USP)Department of Basic Sciences School of Dentistry São Paulo State University (UNESP)Dentistry School University of Marilia (UNIMAR)Pro-Rectory of Research and Graduate Studies University Center of Adamantina (UNIFAI)Rector/President University Center of Adamantina (UNIFAI)Vice-Rector/President University Center of Adamantina (UNIFAI)Graduate Program in Anatomy of Domestic and Wild Animals Faculty of Veterinary Medicine and Animal Science University of São Paulo (FMVZ/USP)Department of Basic Sciences School of Dentistry São Paulo State University (UNESP)Universidade de São Paulo (USP)University of Marilia (UNIMAR)University Center of Adamantina (UNIFAI)Universidade Estadual Paulista (UNESP)Pomini, Karina TorresBuchaim, Daniela VieiraBighetti, Ana Carolina CestariAndreo, Jesus CarlosRosso, Marcelie Priscila de OliveiraEscudero, José Stalin BayasDella Coletta, Bruna BotteonAlcalde, Murilo PrioriDuarte, Marco Antonio HungaroPitol, Dimitrius LeonardoIssa, João Paulo MardeganErvolino, Edilson [UNESP]Moscatel, Matheus Bento MedeirosBellini, Márcia ZilioliSouza, Alexandre Teixeira deSoares, Wendel CleberBuchaim, Rogerio Leone2023-07-29T13:59:15Z2023-07-29T13:59:15Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/polym14194170Polymers, v. 14, n. 19, 2022.2073-4360http://hdl.handle.net/11449/24898810.3390/polym141941702-s2.0-85139870521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolymersinfo:eu-repo/semantics/openAccess2024-04-23T15:22:59Zoai:repositorio.unesp.br:11449/248988Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:36:24.322232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
title |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
spellingShingle |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects Pomini, Karina Torres biomaterial bone repair bone substitute fibrin sealant low-level laser therapy photobiomodulation therapy polymers scaffolds |
title_short |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
title_full |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
title_fullStr |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
title_full_unstemmed |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
title_sort |
Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects |
author |
Pomini, Karina Torres |
author_facet |
Pomini, Karina Torres Buchaim, Daniela Vieira Bighetti, Ana Carolina Cestari Andreo, Jesus Carlos Rosso, Marcelie Priscila de Oliveira Escudero, José Stalin Bayas Della Coletta, Bruna Botteon Alcalde, Murilo Priori Duarte, Marco Antonio Hungaro Pitol, Dimitrius Leonardo Issa, João Paulo Mardegan Ervolino, Edilson [UNESP] Moscatel, Matheus Bento Medeiros Bellini, Márcia Zilioli Souza, Alexandre Teixeira de Soares, Wendel Cleber Buchaim, Rogerio Leone |
author_role |
author |
author2 |
Buchaim, Daniela Vieira Bighetti, Ana Carolina Cestari Andreo, Jesus Carlos Rosso, Marcelie Priscila de Oliveira Escudero, José Stalin Bayas Della Coletta, Bruna Botteon Alcalde, Murilo Priori Duarte, Marco Antonio Hungaro Pitol, Dimitrius Leonardo Issa, João Paulo Mardegan Ervolino, Edilson [UNESP] Moscatel, Matheus Bento Medeiros Bellini, Márcia Zilioli Souza, Alexandre Teixeira de Soares, Wendel Cleber Buchaim, Rogerio Leone |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) University of Marilia (UNIMAR) University Center of Adamantina (UNIFAI) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Pomini, Karina Torres Buchaim, Daniela Vieira Bighetti, Ana Carolina Cestari Andreo, Jesus Carlos Rosso, Marcelie Priscila de Oliveira Escudero, José Stalin Bayas Della Coletta, Bruna Botteon Alcalde, Murilo Priori Duarte, Marco Antonio Hungaro Pitol, Dimitrius Leonardo Issa, João Paulo Mardegan Ervolino, Edilson [UNESP] Moscatel, Matheus Bento Medeiros Bellini, Márcia Zilioli Souza, Alexandre Teixeira de Soares, Wendel Cleber Buchaim, Rogerio Leone |
dc.subject.por.fl_str_mv |
biomaterial bone repair bone substitute fibrin sealant low-level laser therapy photobiomodulation therapy polymers scaffolds |
topic |
biomaterial bone repair bone substitute fibrin sealant low-level laser therapy photobiomodulation therapy polymers scaffolds |
description |
In this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-07-29T13:59:15Z 2023-07-29T13:59:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/polym14194170 Polymers, v. 14, n. 19, 2022. 2073-4360 http://hdl.handle.net/11449/248988 10.3390/polym14194170 2-s2.0-85139870521 |
url |
http://dx.doi.org/10.3390/polym14194170 http://hdl.handle.net/11449/248988 |
identifier_str_mv |
Polymers, v. 14, n. 19, 2022. 2073-4360 10.3390/polym14194170 2-s2.0-85139870521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Polymers |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128386771451904 |