Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects

Detalhes bibliográficos
Autor(a) principal: Pomini, Karina Torres
Data de Publicação: 2022
Outros Autores: Buchaim, Daniela Vieira, Bighetti, Ana Carolina Cestari, Andreo, Jesus Carlos, Rosso, Marcelie Priscila de Oliveira, Escudero, José Stalin Bayas, Della Coletta, Bruna Botteon, Alcalde, Murilo Priori, Duarte, Marco Antonio Hungaro, Pitol, Dimitrius Leonardo, Issa, João Paulo Mardegan, Ervolino, Edilson [UNESP], Moscatel, Matheus Bento Medeiros, Bellini, Márcia Zilioli, Souza, Alexandre Teixeira de, Soares, Wendel Cleber, Buchaim, Rogerio Leone
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/polym14194170
http://hdl.handle.net/11449/248988
Resumo: In this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T.
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spelling Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defectsbiomaterialbone repairbone substitutefibrin sealantlow-level laser therapyphotobiomodulation therapypolymersscaffoldsIn this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T.Department of Biological Sciences Bauru School of Dentistry FOB/USP University of São PauloStructural and Functional Interactions in Rehabilitation Postgraduate Department University of Marilia (UNIMAR)Teaching and Research Coordination of the Medical School University Center of Adamantina (UNIFAI)Department of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (FOB/USP)Department of Basic and Oral Biology School of Dentistry of Ribeirão Preto University of São Paulo (FORP/USP)Department of Basic Sciences School of Dentistry São Paulo State University (UNESP)Dentistry School University of Marilia (UNIMAR)Pro-Rectory of Research and Graduate Studies University Center of Adamantina (UNIFAI)Rector/President University Center of Adamantina (UNIFAI)Vice-Rector/President University Center of Adamantina (UNIFAI)Graduate Program in Anatomy of Domestic and Wild Animals Faculty of Veterinary Medicine and Animal Science University of São Paulo (FMVZ/USP)Department of Basic Sciences School of Dentistry São Paulo State University (UNESP)Universidade de São Paulo (USP)University of Marilia (UNIMAR)University Center of Adamantina (UNIFAI)Universidade Estadual Paulista (UNESP)Pomini, Karina TorresBuchaim, Daniela VieiraBighetti, Ana Carolina CestariAndreo, Jesus CarlosRosso, Marcelie Priscila de OliveiraEscudero, José Stalin BayasDella Coletta, Bruna BotteonAlcalde, Murilo PrioriDuarte, Marco Antonio HungaroPitol, Dimitrius LeonardoIssa, João Paulo MardeganErvolino, Edilson [UNESP]Moscatel, Matheus Bento MedeirosBellini, Márcia ZilioliSouza, Alexandre Teixeira deSoares, Wendel CleberBuchaim, Rogerio Leone2023-07-29T13:59:15Z2023-07-29T13:59:15Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/polym14194170Polymers, v. 14, n. 19, 2022.2073-4360http://hdl.handle.net/11449/24898810.3390/polym141941702-s2.0-85139870521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolymersinfo:eu-repo/semantics/openAccess2024-04-23T15:22:59Zoai:repositorio.unesp.br:11449/248988Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:36:24.322232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
title Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
spellingShingle Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
Pomini, Karina Torres
biomaterial
bone repair
bone substitute
fibrin sealant
low-level laser therapy
photobiomodulation therapy
polymers
scaffolds
title_short Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
title_full Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
title_fullStr Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
title_full_unstemmed Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
title_sort Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects
author Pomini, Karina Torres
author_facet Pomini, Karina Torres
Buchaim, Daniela Vieira
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Rosso, Marcelie Priscila de Oliveira
Escudero, José Stalin Bayas
Della Coletta, Bruna Botteon
Alcalde, Murilo Priori
Duarte, Marco Antonio Hungaro
Pitol, Dimitrius Leonardo
Issa, João Paulo Mardegan
Ervolino, Edilson [UNESP]
Moscatel, Matheus Bento Medeiros
Bellini, Márcia Zilioli
Souza, Alexandre Teixeira de
Soares, Wendel Cleber
Buchaim, Rogerio Leone
author_role author
author2 Buchaim, Daniela Vieira
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Rosso, Marcelie Priscila de Oliveira
Escudero, José Stalin Bayas
Della Coletta, Bruna Botteon
Alcalde, Murilo Priori
Duarte, Marco Antonio Hungaro
Pitol, Dimitrius Leonardo
Issa, João Paulo Mardegan
Ervolino, Edilson [UNESP]
Moscatel, Matheus Bento Medeiros
Bellini, Márcia Zilioli
Souza, Alexandre Teixeira de
Soares, Wendel Cleber
Buchaim, Rogerio Leone
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
University of Marilia (UNIMAR)
University Center of Adamantina (UNIFAI)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pomini, Karina Torres
Buchaim, Daniela Vieira
Bighetti, Ana Carolina Cestari
Andreo, Jesus Carlos
Rosso, Marcelie Priscila de Oliveira
Escudero, José Stalin Bayas
Della Coletta, Bruna Botteon
Alcalde, Murilo Priori
Duarte, Marco Antonio Hungaro
Pitol, Dimitrius Leonardo
Issa, João Paulo Mardegan
Ervolino, Edilson [UNESP]
Moscatel, Matheus Bento Medeiros
Bellini, Márcia Zilioli
Souza, Alexandre Teixeira de
Soares, Wendel Cleber
Buchaim, Rogerio Leone
dc.subject.por.fl_str_mv biomaterial
bone repair
bone substitute
fibrin sealant
low-level laser therapy
photobiomodulation therapy
polymers
scaffolds
topic biomaterial
bone repair
bone substitute
fibrin sealant
low-level laser therapy
photobiomodulation therapy
polymers
scaffolds
description In this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-01
2023-07-29T13:59:15Z
2023-07-29T13:59:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/polym14194170
Polymers, v. 14, n. 19, 2022.
2073-4360
http://hdl.handle.net/11449/248988
10.3390/polym14194170
2-s2.0-85139870521
url http://dx.doi.org/10.3390/polym14194170
http://hdl.handle.net/11449/248988
identifier_str_mv Polymers, v. 14, n. 19, 2022.
2073-4360
10.3390/polym14194170
2-s2.0-85139870521
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Polymers
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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