Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.exppara.2016.09.006 http://hdl.handle.net/11449/159199 |
Resumo: | The var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved. |
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Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum populationPlasmodium falciparumVariant surface antigenPfEMP-1AntibodiesAmazonThe var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Inst Biomed Sci, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, BrazilState Univ Sao Paulo, Inst Biotechnol, Al Tecomarias S-N, BR-18607440 Botucatu, SP, BrazilUniv Estadual Campinas, Inst Biol, R Monteiro Lobato 255,Cidade Univ, BR-13083862 Campinas, SP, BrazilUniv Fed Bahia, Hosp Prof Edgard Santos, Med Genet Serv, R Dr Augusto Viana S-N, BR-40110060 Salvador, BA, BrazilState Univ Sao Paulo, Inst Biotechnol, Al Tecomarias S-N, BR-18607440 Botucatu, SP, BrazilFAPESP: 2009/17114-3FAPESP: 2010/50333-8CNPq: 576128/2008-2FAPESP: 2009/53702-7Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade Federal da Bahia (UFBA)Carlos, Bianca C. [UNESP]Fotoran, Wesley L.Menezes, Maria J.Cabral, Fernanda J.Bastos, Marcele F.Costa, Fabio T. M.Sousa-Neto, Jayme A. [UNESP]Ribolla, Paulo E. M. [UNESP]Wunderlich, GerhardFerreira, Marcelo U.2018-11-26T15:37:23Z2018-11-26T15:37:23Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article90-99application/pdfhttp://dx.doi.org/10.1016/j.exppara.2016.09.006Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016.0014-4894http://hdl.handle.net/11449/15919910.1016/j.exppara.2016.09.006WOS:000388059400012WOS000388059400012.pdf35771497484568800000-0001-8735-6090Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Parasitology0,635info:eu-repo/semantics/openAccess2023-11-13T06:12:37Zoai:repositorio.unesp.br:11449/159199Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:08.388555Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
title |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
spellingShingle |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population Carlos, Bianca C. [UNESP] Plasmodium falciparum Variant surface antigen PfEMP-1 Antibodies Amazon |
title_short |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
title_full |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
title_fullStr |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
title_full_unstemmed |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
title_sort |
Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population |
author |
Carlos, Bianca C. [UNESP] |
author_facet |
Carlos, Bianca C. [UNESP] Fotoran, Wesley L. Menezes, Maria J. Cabral, Fernanda J. Bastos, Marcele F. Costa, Fabio T. M. Sousa-Neto, Jayme A. [UNESP] Ribolla, Paulo E. M. [UNESP] Wunderlich, Gerhard Ferreira, Marcelo U. |
author_role |
author |
author2 |
Fotoran, Wesley L. Menezes, Maria J. Cabral, Fernanda J. Bastos, Marcele F. Costa, Fabio T. M. Sousa-Neto, Jayme A. [UNESP] Ribolla, Paulo E. M. [UNESP] Wunderlich, Gerhard Ferreira, Marcelo U. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade Federal da Bahia (UFBA) |
dc.contributor.author.fl_str_mv |
Carlos, Bianca C. [UNESP] Fotoran, Wesley L. Menezes, Maria J. Cabral, Fernanda J. Bastos, Marcele F. Costa, Fabio T. M. Sousa-Neto, Jayme A. [UNESP] Ribolla, Paulo E. M. [UNESP] Wunderlich, Gerhard Ferreira, Marcelo U. |
dc.subject.por.fl_str_mv |
Plasmodium falciparum Variant surface antigen PfEMP-1 Antibodies Amazon |
topic |
Plasmodium falciparum Variant surface antigen PfEMP-1 Antibodies Amazon |
description |
The var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T15:37:23Z 2018-11-26T15:37:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.exppara.2016.09.006 Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016. 0014-4894 http://hdl.handle.net/11449/159199 10.1016/j.exppara.2016.09.006 WOS:000388059400012 WOS000388059400012.pdf 3577149748456880 0000-0001-8735-6090 |
url |
http://dx.doi.org/10.1016/j.exppara.2016.09.006 http://hdl.handle.net/11449/159199 |
identifier_str_mv |
Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016. 0014-4894 10.1016/j.exppara.2016.09.006 WOS:000388059400012 WOS000388059400012.pdf 3577149748456880 0000-0001-8735-6090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental Parasitology 0,635 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
90-99 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128830020255744 |