Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population

Detalhes bibliográficos
Autor(a) principal: Carlos, Bianca C. [UNESP]
Data de Publicação: 2016
Outros Autores: Fotoran, Wesley L., Menezes, Maria J., Cabral, Fernanda J., Bastos, Marcele F., Costa, Fabio T. M., Sousa-Neto, Jayme A. [UNESP], Ribolla, Paulo E. M. [UNESP], Wunderlich, Gerhard, Ferreira, Marcelo U.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.exppara.2016.09.006
http://hdl.handle.net/11449/159199
Resumo: The var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved.
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spelling Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum populationPlasmodium falciparumVariant surface antigenPfEMP-1AntibodiesAmazonThe var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Inst Biomed Sci, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, BrazilState Univ Sao Paulo, Inst Biotechnol, Al Tecomarias S-N, BR-18607440 Botucatu, SP, BrazilUniv Estadual Campinas, Inst Biol, R Monteiro Lobato 255,Cidade Univ, BR-13083862 Campinas, SP, BrazilUniv Fed Bahia, Hosp Prof Edgard Santos, Med Genet Serv, R Dr Augusto Viana S-N, BR-40110060 Salvador, BA, BrazilState Univ Sao Paulo, Inst Biotechnol, Al Tecomarias S-N, BR-18607440 Botucatu, SP, BrazilFAPESP: 2009/17114-3FAPESP: 2010/50333-8CNPq: 576128/2008-2FAPESP: 2009/53702-7Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade Federal da Bahia (UFBA)Carlos, Bianca C. [UNESP]Fotoran, Wesley L.Menezes, Maria J.Cabral, Fernanda J.Bastos, Marcele F.Costa, Fabio T. M.Sousa-Neto, Jayme A. [UNESP]Ribolla, Paulo E. M. [UNESP]Wunderlich, GerhardFerreira, Marcelo U.2018-11-26T15:37:23Z2018-11-26T15:37:23Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article90-99application/pdfhttp://dx.doi.org/10.1016/j.exppara.2016.09.006Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016.0014-4894http://hdl.handle.net/11449/15919910.1016/j.exppara.2016.09.006WOS:000388059400012WOS000388059400012.pdf35771497484568800000-0001-8735-6090Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Parasitology0,635info:eu-repo/semantics/openAccess2023-11-13T06:12:37Zoai:repositorio.unesp.br:11449/159199Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:35:08.388555Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
title Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
spellingShingle Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
Carlos, Bianca C. [UNESP]
Plasmodium falciparum
Variant surface antigen
PfEMP-1
Antibodies
Amazon
title_short Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
title_full Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
title_fullStr Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
title_full_unstemmed Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
title_sort Expressed var gene repertoire and variant surface antigen diversity in a shrinking Plasmodium falciparum population
author Carlos, Bianca C. [UNESP]
author_facet Carlos, Bianca C. [UNESP]
Fotoran, Wesley L.
Menezes, Maria J.
Cabral, Fernanda J.
Bastos, Marcele F.
Costa, Fabio T. M.
Sousa-Neto, Jayme A. [UNESP]
Ribolla, Paulo E. M. [UNESP]
Wunderlich, Gerhard
Ferreira, Marcelo U.
author_role author
author2 Fotoran, Wesley L.
Menezes, Maria J.
Cabral, Fernanda J.
Bastos, Marcele F.
Costa, Fabio T. M.
Sousa-Neto, Jayme A. [UNESP]
Ribolla, Paulo E. M. [UNESP]
Wunderlich, Gerhard
Ferreira, Marcelo U.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal da Bahia (UFBA)
dc.contributor.author.fl_str_mv Carlos, Bianca C. [UNESP]
Fotoran, Wesley L.
Menezes, Maria J.
Cabral, Fernanda J.
Bastos, Marcele F.
Costa, Fabio T. M.
Sousa-Neto, Jayme A. [UNESP]
Ribolla, Paulo E. M. [UNESP]
Wunderlich, Gerhard
Ferreira, Marcelo U.
dc.subject.por.fl_str_mv Plasmodium falciparum
Variant surface antigen
PfEMP-1
Antibodies
Amazon
topic Plasmodium falciparum
Variant surface antigen
PfEMP-1
Antibodies
Amazon
description The var gene-encoded erythrocyte membrane protein-1 of Plasmodium falciparum (PfEMP-1) is the main variant surface antigen (VSA) expressed on infected erythrocytes. The rate at which antibody responses to VSA expressed by circulating parasites are acquired depends on the size of the local VSA repertoire and the frequency of exposure to new VSA. Because parasites from areas with declining malaria endemicity, such as the Amazon, typically express a restricted PfEMP-1 repertoire, we hypothesized that Amazonians would rapidly acquire antibodies to most locally circulating VSA. Consistent with our expectations, the analysis of 5878 sequence tags expressed by 10 local P. falciparum samples revealed little PfEMP-1 DBL1 alpha domain diversity. Among the most commonly expressed DBL1 alpha types, 45% were shared by two or more independent parasite lines. Nevertheless, Amazonians displayed major gaps in their repertoire of anti-VSA antibodies, although the breadth of anti-VSA antibody responses correlated positively with their cumulative exposure to malaria. We found little antibody cross-reactivity even when testing VSA from related parasites expressing the same dominant DBL1 alpha types. We conclude that variant-specific immunity to P. falciparum VSAs develops slowly despite the relatively restricted PfEMP-1 repertoire found in low-endemicity settings. (C) 2016 Elsevier Inc. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
2018-11-26T15:37:23Z
2018-11-26T15:37:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.exppara.2016.09.006
Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016.
0014-4894
http://hdl.handle.net/11449/159199
10.1016/j.exppara.2016.09.006
WOS:000388059400012
WOS000388059400012.pdf
3577149748456880
0000-0001-8735-6090
url http://dx.doi.org/10.1016/j.exppara.2016.09.006
http://hdl.handle.net/11449/159199
identifier_str_mv Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 170, p. 90-99, 2016.
0014-4894
10.1016/j.exppara.2016.09.006
WOS:000388059400012
WOS000388059400012.pdf
3577149748456880
0000-0001-8735-6090
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Parasitology
0,635
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 90-99
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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