Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells

Detalhes bibliográficos
Autor(a) principal: Bernardo, Victoria Simões [UNESP]
Data de Publicação: 2022
Outros Autores: Torres, Flaviene Felix [UNESP], de Paula, Carla Peres, da Silva, João Pedro Maia de Oliveira, de Almeida, Eduardo Alves, da Cunha, Anderson Ferreira, da Silva, Danilo Grünig Humberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/genes13122368
http://hdl.handle.net/11449/246515
Resumo: This study aimed to establish the importance of ergothioneine (ERT) in the erythroid adaptation mechanisms by appraising the expression levels of redox-related genes associated with the PI3K/AKT/FoxO3 and Nrf2-ARE pathways using K562 cells induced to erythroid differentiation and H2O2-oxidative stress. Cell viability and gene expression were evaluated. Two concentrations of ERT were assessed, 1 nM (C1) and 100 µM (C2), with and without stress induction (100 µM H2O2). Assessments were made in three periods of the cellular differentiation process (D0, D2, and D4). The C1 treatment promoted the induction of FOXO3 (D0 and 2), PSMB5, and 6 expressions (D4); C1 + H2O2 treatment showed the highest levels of NRF2 transcripts, KEAP1 (D0), YWHAQ (D2 and 4), PSMB5 (D2) and PSMB6 (D4); and C2 + H2O2 (D2) an increase in FOXO3 and MST1 expression, with a decrease of YWHAQ and NRF2 was observed. in C2 + H2O2 (D2) an increase in FOXO3 and MST1, with a decrease in YWHAQ and NRF2 was observed All ERT treatments increased gamma-globin expression. Statistical multivariate analyzes highlighted that the Nrf2-ARE pathway presented a greater contribution in the production of PRDX1, SOD1, CAT, and PSBM5 mRNAs, whereas the PI3K/AKT/FoxO3 pathway was associated with the PRDX2 and TRX transcripts. In conclusion, ERT presented a cytoprotective action through Nrf2 and FoxO3, with the latter seeming to contribute to erythroid proliferation/differentiation.
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spelling Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cellsantioxidantsproteasomeredox adaptationtherapeutic agentThis study aimed to establish the importance of ergothioneine (ERT) in the erythroid adaptation mechanisms by appraising the expression levels of redox-related genes associated with the PI3K/AKT/FoxO3 and Nrf2-ARE pathways using K562 cells induced to erythroid differentiation and H2O2-oxidative stress. Cell viability and gene expression were evaluated. Two concentrations of ERT were assessed, 1 nM (C1) and 100 µM (C2), with and without stress induction (100 µM H2O2). Assessments were made in three periods of the cellular differentiation process (D0, D2, and D4). The C1 treatment promoted the induction of FOXO3 (D0 and 2), PSMB5, and 6 expressions (D4); C1 + H2O2 treatment showed the highest levels of NRF2 transcripts, KEAP1 (D0), YWHAQ (D2 and 4), PSMB5 (D2) and PSMB6 (D4); and C2 + H2O2 (D2) an increase in FOXO3 and MST1 expression, with a decrease of YWHAQ and NRF2 was observed. in C2 + H2O2 (D2) an increase in FOXO3 and MST1, with a decrease in YWHAQ and NRF2 was observed All ERT treatments increased gamma-globin expression. Statistical multivariate analyzes highlighted that the Nrf2-ARE pathway presented a greater contribution in the production of PRDX1, SOD1, CAT, and PSBM5 mRNAs, whereas the PI3K/AKT/FoxO3 pathway was associated with the PRDX2 and TRX transcripts. In conclusion, ERT presented a cytoprotective action through Nrf2 and FoxO3, with the latter seeming to contribute to erythroid proliferation/differentiation.Department of Biology Universidade Estadual Paulista (UNESP), SPDepartment of Genetics and Evolution Universidade Federal de São Carlos (UFSCar), SPDepartment of Natural Sciences Fundação Universidade Regional de Blumenau (FURB), SCUniversidade Federal de Mato Grosso do Sul (CPTL/UFMS), Campus de Três Lagoas, MSDepartment of Biology Universidade Estadual Paulista (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Fundação Universidade Regional de Blumenau (FURB)Universidade Federal de Mato Grosso do Sul (UFMS)Bernardo, Victoria Simões [UNESP]Torres, Flaviene Felix [UNESP]de Paula, Carla Peresda Silva, João Pedro Maia de Oliveirade Almeida, Eduardo Alvesda Cunha, Anderson Ferreirada Silva, Danilo Grünig Humberto2023-07-29T12:43:07Z2023-07-29T12:43:07Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/genes13122368Genes, v. 13, n. 12, 2022.2073-4425http://hdl.handle.net/11449/24651510.3390/genes131223682-s2.0-85144517144Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2023-07-29T12:43:07Zoai:repositorio.unesp.br:11449/246515Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:58.268950Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
title Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
spellingShingle Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
Bernardo, Victoria Simões [UNESP]
antioxidants
proteasome
redox adaptation
therapeutic agent
title_short Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
title_full Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
title_fullStr Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
title_full_unstemmed Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
title_sort Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells
author Bernardo, Victoria Simões [UNESP]
author_facet Bernardo, Victoria Simões [UNESP]
Torres, Flaviene Felix [UNESP]
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
author_role author
author2 Torres, Flaviene Felix [UNESP]
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
Fundação Universidade Regional de Blumenau (FURB)
Universidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.author.fl_str_mv Bernardo, Victoria Simões [UNESP]
Torres, Flaviene Felix [UNESP]
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
dc.subject.por.fl_str_mv antioxidants
proteasome
redox adaptation
therapeutic agent
topic antioxidants
proteasome
redox adaptation
therapeutic agent
description This study aimed to establish the importance of ergothioneine (ERT) in the erythroid adaptation mechanisms by appraising the expression levels of redox-related genes associated with the PI3K/AKT/FoxO3 and Nrf2-ARE pathways using K562 cells induced to erythroid differentiation and H2O2-oxidative stress. Cell viability and gene expression were evaluated. Two concentrations of ERT were assessed, 1 nM (C1) and 100 µM (C2), with and without stress induction (100 µM H2O2). Assessments were made in three periods of the cellular differentiation process (D0, D2, and D4). The C1 treatment promoted the induction of FOXO3 (D0 and 2), PSMB5, and 6 expressions (D4); C1 + H2O2 treatment showed the highest levels of NRF2 transcripts, KEAP1 (D0), YWHAQ (D2 and 4), PSMB5 (D2) and PSMB6 (D4); and C2 + H2O2 (D2) an increase in FOXO3 and MST1 expression, with a decrease of YWHAQ and NRF2 was observed. in C2 + H2O2 (D2) an increase in FOXO3 and MST1, with a decrease in YWHAQ and NRF2 was observed All ERT treatments increased gamma-globin expression. Statistical multivariate analyzes highlighted that the Nrf2-ARE pathway presented a greater contribution in the production of PRDX1, SOD1, CAT, and PSBM5 mRNAs, whereas the PI3K/AKT/FoxO3 pathway was associated with the PRDX2 and TRX transcripts. In conclusion, ERT presented a cytoprotective action through Nrf2 and FoxO3, with the latter seeming to contribute to erythroid proliferation/differentiation.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-01
2023-07-29T12:43:07Z
2023-07-29T12:43:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/genes13122368
Genes, v. 13, n. 12, 2022.
2073-4425
http://hdl.handle.net/11449/246515
10.3390/genes13122368
2-s2.0-85144517144
url http://dx.doi.org/10.3390/genes13122368
http://hdl.handle.net/11449/246515
identifier_str_mv Genes, v. 13, n. 12, 2022.
2073-4425
10.3390/genes13122368
2-s2.0-85144517144
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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