Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis

Detalhes bibliográficos
Autor(a) principal: Saad-Hossne, Rogério [UNESP]
Data de Publicação: 2006
Outros Autores: Prado, René Gamberini [UNESP], Hossne, William Saad [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502006000300006
http://hdl.handle.net/11449/68871
Resumo: Purpose: To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death. Methods: The VX2 tumor cells (107 cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy. Results: Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions. Conclusion: Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.
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spelling Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysisAcetic acidAspirinCarcinomaIn vitroacetic acidacetylsalicylic acidbicarbonatesodium chloridetrypan blueanimal cellcarcinoma cellcell deathcell suspensioncell viabilitycontrolled studycorrelation analysisdrug blood levelin vitro studyincubation timemicroscopynonhumanAcetic AcidAnimalsCell DeathCell SurvivalDrug CombinationsDrug Screening Assays, AntitumorIndicators and ReagentsMicroscopy, FluorescenceNeoplasms, ExperimentalRabbitsPurpose: To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death. Methods: The VX2 tumor cells (107 cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy. Results: Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions. Conclusion: Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.Department of Surgery and Orthopedics Paulista State University Botucatu Medical SchoolPaulista State University Botucatu Medical School UNESPDepartamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu UNESP, 18618-970 Botucatu - SPDepartment of Surgery and Orthopedics Paulista State University Botucatu Medical SchoolPaulista State University Botucatu Medical School UNESPDepartamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu UNESP, 18618-970 Botucatu - SPUniversidade Estadual Paulista (Unesp)Saad-Hossne, Rogério [UNESP]Prado, René Gamberini [UNESP]Hossne, William Saad [UNESP]2014-05-27T11:21:51Z2014-05-27T11:21:51Z2006-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article151-154application/pdfhttp://dx.doi.org/10.1590/S0102-86502006000300006Acta Cirurgica Brasileira, v. 21, n. 3, p. 151-154, 2006.0102-86501678-2674http://hdl.handle.net/11449/6887110.1590/S0102-86502006000300006S0102-865020060003000062-s2.0-337449100622-s2.0-33744910062.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-08-14T14:19:30Zoai:repositorio.unesp.br:11449/68871Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T14:19:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
title Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
spellingShingle Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
Saad-Hossne, Rogério [UNESP]
Acetic acid
Aspirin
Carcinoma
In vitro
acetic acid
acetylsalicylic acid
bicarbonate
sodium chloride
trypan blue
animal cell
carcinoma cell
cell death
cell suspension
cell viability
controlled study
correlation analysis
drug blood level
in vitro study
incubation time
microscopy
nonhuman
Acetic Acid
Animals
Cell Death
Cell Survival
Drug Combinations
Drug Screening Assays, Antitumor
Indicators and Reagents
Microscopy, Fluorescence
Neoplasms, Experimental
Rabbits
title_short Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
title_full Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
title_fullStr Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
title_full_unstemmed Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
title_sort Effects of acetylsalicylic acid and acetic acid solutions in VX2 carcinoma cells. In vitro analysis
author Saad-Hossne, Rogério [UNESP]
author_facet Saad-Hossne, Rogério [UNESP]
Prado, René Gamberini [UNESP]
Hossne, William Saad [UNESP]
author_role author
author2 Prado, René Gamberini [UNESP]
Hossne, William Saad [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Saad-Hossne, Rogério [UNESP]
Prado, René Gamberini [UNESP]
Hossne, William Saad [UNESP]
dc.subject.por.fl_str_mv Acetic acid
Aspirin
Carcinoma
In vitro
acetic acid
acetylsalicylic acid
bicarbonate
sodium chloride
trypan blue
animal cell
carcinoma cell
cell death
cell suspension
cell viability
controlled study
correlation analysis
drug blood level
in vitro study
incubation time
microscopy
nonhuman
Acetic Acid
Animals
Cell Death
Cell Survival
Drug Combinations
Drug Screening Assays, Antitumor
Indicators and Reagents
Microscopy, Fluorescence
Neoplasms, Experimental
Rabbits
topic Acetic acid
Aspirin
Carcinoma
In vitro
acetic acid
acetylsalicylic acid
bicarbonate
sodium chloride
trypan blue
animal cell
carcinoma cell
cell death
cell suspension
cell viability
controlled study
correlation analysis
drug blood level
in vitro study
incubation time
microscopy
nonhuman
Acetic Acid
Animals
Cell Death
Cell Survival
Drug Combinations
Drug Screening Assays, Antitumor
Indicators and Reagents
Microscopy, Fluorescence
Neoplasms, Experimental
Rabbits
description Purpose: To analyze, in vitro, the effects of acetylsalicylic acid (aspirin) and acetic acid solutions on VX2 carcinoma cells in suspension and to examine the correlation between these effects and neoplastic cell death. Methods: The VX2 tumor cells (107 cells/ml) were incubated in solutions containing differing concentrations (2.5% and 5%) of either acetylsalicylic acid or acetic acid, or in saline solution (controls). Every five minutes, cell viability was tested (using the trypan blue test) and analyzed under light microscopy. Results: Tumor cell viability (in %) decreased progressively and, by 30 minutes, neoplastic cell death had occurred in all solutions. Conclusion: Based on this experimental model and the methodology employed, we conclude that these solutions cause neoplastic cell death in vitro.
publishDate 2006
dc.date.none.fl_str_mv 2006-05-01
2014-05-27T11:21:51Z
2014-05-27T11:21:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502006000300006
Acta Cirurgica Brasileira, v. 21, n. 3, p. 151-154, 2006.
0102-8650
1678-2674
http://hdl.handle.net/11449/68871
10.1590/S0102-86502006000300006
S0102-86502006000300006
2-s2.0-33744910062
2-s2.0-33744910062.pdf
url http://dx.doi.org/10.1590/S0102-86502006000300006
http://hdl.handle.net/11449/68871
identifier_str_mv Acta Cirurgica Brasileira, v. 21, n. 3, p. 151-154, 2006.
0102-8650
1678-2674
10.1590/S0102-86502006000300006
S0102-86502006000300006
2-s2.0-33744910062
2-s2.0-33744910062.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirúrgica Brasileira
0.933
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 151-154
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128185834930176

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