Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2012/RSBO---v9---n01/725482 http://hdl.handle.net/11449/140847 |
Resumo: | Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. |
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Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure timeForensic dentistryForensic anthropologyForensic sciencesIntroduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed.Universidade de São Paulo (USP), Faculdade de Odontologia de Ribeirão Preto (FORP), Departamento de Clínica Infantil e Odontologia Preventiva e Social, Ribeirão Preto, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências (IBB), Departamento de Genética, Botucatu, SP, BrasilUniversidade de São Paulo (USP), Faculdade de Odontologia (FO), São Paulo, SP, BrasilUniversidade de São Paulo (USP), Faculdade de Odontologia de Bauru (FOB), Bauru, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências (IBB), Departamento de Genética, Botucatu, SP, BrasilUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Silva, Ricardo Henrique Alves daQuiezi, Rodrigo Gonçalves [UNESP]Bertolacini, Claudia Danielli Pereira [UNESP]Carvalho, Suzana Papile MacielGasque, Kellen Cristina da SilvaSilva, Carina Thais de Almeida eBicudo, Lucilene Arilho Ribeiro2016-07-07T12:35:41Z2016-07-07T12:35:41Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article67-73application/pdfhttp://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2012/RSBO---v9---n01/725482RSBO. Revista Sul-Brasileira de Odontologia, v. 9, n. 1, p. 67-73, 2012.1806-7727http://hdl.handle.net/11449/140847ISSN1806-7727-2012-09-01-67-73.pdfCurrículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRSBO. Revista Sul-Brasileira de Odontologiainfo:eu-repo/semantics/openAccess2023-11-08T06:08:44Zoai:repositorio.unesp.br:11449/140847Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:08:22.803981Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
title |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
spellingShingle |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time Silva, Ricardo Henrique Alves da Forensic dentistry Forensic anthropology Forensic sciences |
title_short |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
title_full |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
title_fullStr |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
title_full_unstemmed |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
title_sort |
Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
author |
Silva, Ricardo Henrique Alves da |
author_facet |
Silva, Ricardo Henrique Alves da Quiezi, Rodrigo Gonçalves [UNESP] Bertolacini, Claudia Danielli Pereira [UNESP] Carvalho, Suzana Papile Maciel Gasque, Kellen Cristina da Silva Silva, Carina Thais de Almeida e Bicudo, Lucilene Arilho Ribeiro |
author_role |
author |
author2 |
Quiezi, Rodrigo Gonçalves [UNESP] Bertolacini, Claudia Danielli Pereira [UNESP] Carvalho, Suzana Papile Maciel Gasque, Kellen Cristina da Silva Silva, Carina Thais de Almeida e Bicudo, Lucilene Arilho Ribeiro |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Ricardo Henrique Alves da Quiezi, Rodrigo Gonçalves [UNESP] Bertolacini, Claudia Danielli Pereira [UNESP] Carvalho, Suzana Papile Maciel Gasque, Kellen Cristina da Silva Silva, Carina Thais de Almeida e Bicudo, Lucilene Arilho Ribeiro |
dc.subject.por.fl_str_mv |
Forensic dentistry Forensic anthropology Forensic sciences |
topic |
Forensic dentistry Forensic anthropology Forensic sciences |
description |
Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2016-07-07T12:35:41Z 2016-07-07T12:35:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2012/RSBO---v9---n01/725482 RSBO. Revista Sul-Brasileira de Odontologia, v. 9, n. 1, p. 67-73, 2012. 1806-7727 http://hdl.handle.net/11449/140847 ISSN1806-7727-2012-09-01-67-73.pdf |
url |
http://www.univille.edu.br/pt-BR/departamentos/odontologia/revista-rsbo/edicoes--2012/RSBO---v9---n01/725482 http://hdl.handle.net/11449/140847 |
identifier_str_mv |
RSBO. Revista Sul-Brasileira de Odontologia, v. 9, n. 1, p. 67-73, 2012. 1806-7727 ISSN1806-7727-2012-09-01-67-73.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RSBO. Revista Sul-Brasileira de Odontologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
67-73 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128760763908096 |