Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test

Detalhes bibliográficos
Autor(a) principal: Mazzeo, Dânia Elisa Christofoletti [UNESP]
Data de Publicação: 2015
Outros Autores: Fernandes, Thaís Cristina Casimiro [UNESP], Levy, Carlos Emílio, Fontanetti, Carmem Silvia [UNESP], Marin-Morales, Maria Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ecolind.2015.03.026
http://hdl.handle.net/11449/177339
Resumo: Appropriate final disposal of sewage sludge (SS) generated by wastewater treatment plants (WWTP) has been considered a serious environmental problem, but also a viable alternative to be applied in agriculture, once SS is rich in organic matter and nutrients. However, SS can be a source of contamination of several toxic agents. Therefore, its use in agriculture requires special care to avoid possible damage to the environment and exposed organisms. Detoxification of toxic wastes can be performed using the monitored natural attenuation, which involves biological, physical and chemical processes that frequently occur in the environment. This study aimed to assess the feasibility of decontaminating SS after different periods of monitored natural attenuation. To this end, samples of SS and associations of soil/SS with proportions of 10, 25 and 50% SS were buried for 0, 2, 6 and 12 months in holes prepared in a place free of contamination. Allium cepa was used as an indicator to assess the efficiency of the natural attenuation process. According to chemical analysis, the SS samples presented a high concentration of m- and p-cresol, especially for samples analyzed after 0 or 2 months of natural attenuation. The microorganisms present in the SS belonged to 17 different genera of bacteria, which varied in the microbial composition among samples. Both, raw SS and aqueous SS extracts induced DNA damage in A. cepa, even when associated with soil. However, this effect was observed to decline during the attenuation period, although significant effects were detected for the highest tested concentration (100% SS) even at the end of this process. These results thus indicated the necessity of applying a stabilization process associating SS and soil for a period of at least 12 months and showed that the studied raw SS is not a viable material for use as a soil reconditioner, even after natural attenuation. A. cepa test proved to be a useful tool to assess the efficiency of SS detoxification process. Therefore, we suggest that the application of SS in agriculture should be approached with caution and that the SS must be previously submitted to methodologies that evaluate its toxic potential.
id UNSP_02c4cc3837cbcc1ebd807eafb75895c7
oai_identifier_str oai:repositorio.unesp.br:11449/177339
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa testChromosomal aberrationCytotoxicityGenotoxicityMicronucleusSolid wasteWaste managementAppropriate final disposal of sewage sludge (SS) generated by wastewater treatment plants (WWTP) has been considered a serious environmental problem, but also a viable alternative to be applied in agriculture, once SS is rich in organic matter and nutrients. However, SS can be a source of contamination of several toxic agents. Therefore, its use in agriculture requires special care to avoid possible damage to the environment and exposed organisms. Detoxification of toxic wastes can be performed using the monitored natural attenuation, which involves biological, physical and chemical processes that frequently occur in the environment. This study aimed to assess the feasibility of decontaminating SS after different periods of monitored natural attenuation. To this end, samples of SS and associations of soil/SS with proportions of 10, 25 and 50% SS were buried for 0, 2, 6 and 12 months in holes prepared in a place free of contamination. Allium cepa was used as an indicator to assess the efficiency of the natural attenuation process. According to chemical analysis, the SS samples presented a high concentration of m- and p-cresol, especially for samples analyzed after 0 or 2 months of natural attenuation. The microorganisms present in the SS belonged to 17 different genera of bacteria, which varied in the microbial composition among samples. Both, raw SS and aqueous SS extracts induced DNA damage in A. cepa, even when associated with soil. However, this effect was observed to decline during the attenuation period, although significant effects were detected for the highest tested concentration (100% SS) even at the end of this process. These results thus indicated the necessity of applying a stabilization process associating SS and soil for a period of at least 12 months and showed that the studied raw SS is not a viable material for use as a soil reconditioner, even after natural attenuation. A. cepa test proved to be a useful tool to assess the efficiency of SS detoxification process. Therefore, we suggest that the application of SS in agriculture should be approached with caution and that the SS must be previously submitted to methodologies that evaluate its toxic potential.Department of Biology, Institute of Biosciences, UNESP - Univ Estadual Paulista, Av. 24-A, 1515Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas-UNICAMP, Rua Alexander Fleming, 105Department of Biology, Institute of Biosciences, UNESP - Univ Estadual Paulista, Av. 24-A, 1515Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Mazzeo, Dânia Elisa Christofoletti [UNESP]Fernandes, Thaís Cristina Casimiro [UNESP]Levy, Carlos EmílioFontanetti, Carmem Silvia [UNESP]Marin-Morales, Maria Aparecida [UNESP]2018-12-11T17:25:01Z2018-12-11T17:25:01Z2015-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article60-69application/pdfhttp://dx.doi.org/10.1016/j.ecolind.2015.03.026Ecological Indicators, v. 56, p. 60-69.1470-160Xhttp://hdl.handle.net/11449/17733910.1016/j.ecolind.2015.03.0262-s2.0-849284739822-s2.0-84928473982.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcological Indicatorsinfo:eu-repo/semantics/openAccess2023-12-27T06:17:58Zoai:repositorio.unesp.br:11449/177339Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:25:25.557177Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
title Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
spellingShingle Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
Mazzeo, Dânia Elisa Christofoletti [UNESP]
Chromosomal aberration
Cytotoxicity
Genotoxicity
Micronucleus
Solid waste
Waste management
title_short Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
title_full Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
title_fullStr Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
title_full_unstemmed Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
title_sort Monitoring the natural attenuation of a sewage sludge toxicity using the Allium cepa test
author Mazzeo, Dânia Elisa Christofoletti [UNESP]
author_facet Mazzeo, Dânia Elisa Christofoletti [UNESP]
Fernandes, Thaís Cristina Casimiro [UNESP]
Levy, Carlos Emílio
Fontanetti, Carmem Silvia [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
author_role author
author2 Fernandes, Thaís Cristina Casimiro [UNESP]
Levy, Carlos Emílio
Fontanetti, Carmem Silvia [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Mazzeo, Dânia Elisa Christofoletti [UNESP]
Fernandes, Thaís Cristina Casimiro [UNESP]
Levy, Carlos Emílio
Fontanetti, Carmem Silvia [UNESP]
Marin-Morales, Maria Aparecida [UNESP]
dc.subject.por.fl_str_mv Chromosomal aberration
Cytotoxicity
Genotoxicity
Micronucleus
Solid waste
Waste management
topic Chromosomal aberration
Cytotoxicity
Genotoxicity
Micronucleus
Solid waste
Waste management
description Appropriate final disposal of sewage sludge (SS) generated by wastewater treatment plants (WWTP) has been considered a serious environmental problem, but also a viable alternative to be applied in agriculture, once SS is rich in organic matter and nutrients. However, SS can be a source of contamination of several toxic agents. Therefore, its use in agriculture requires special care to avoid possible damage to the environment and exposed organisms. Detoxification of toxic wastes can be performed using the monitored natural attenuation, which involves biological, physical and chemical processes that frequently occur in the environment. This study aimed to assess the feasibility of decontaminating SS after different periods of monitored natural attenuation. To this end, samples of SS and associations of soil/SS with proportions of 10, 25 and 50% SS were buried for 0, 2, 6 and 12 months in holes prepared in a place free of contamination. Allium cepa was used as an indicator to assess the efficiency of the natural attenuation process. According to chemical analysis, the SS samples presented a high concentration of m- and p-cresol, especially for samples analyzed after 0 or 2 months of natural attenuation. The microorganisms present in the SS belonged to 17 different genera of bacteria, which varied in the microbial composition among samples. Both, raw SS and aqueous SS extracts induced DNA damage in A. cepa, even when associated with soil. However, this effect was observed to decline during the attenuation period, although significant effects were detected for the highest tested concentration (100% SS) even at the end of this process. These results thus indicated the necessity of applying a stabilization process associating SS and soil for a period of at least 12 months and showed that the studied raw SS is not a viable material for use as a soil reconditioner, even after natural attenuation. A. cepa test proved to be a useful tool to assess the efficiency of SS detoxification process. Therefore, we suggest that the application of SS in agriculture should be approached with caution and that the SS must be previously submitted to methodologies that evaluate its toxic potential.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
2018-12-11T17:25:01Z
2018-12-11T17:25:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ecolind.2015.03.026
Ecological Indicators, v. 56, p. 60-69.
1470-160X
http://hdl.handle.net/11449/177339
10.1016/j.ecolind.2015.03.026
2-s2.0-84928473982
2-s2.0-84928473982.pdf
url http://dx.doi.org/10.1016/j.ecolind.2015.03.026
http://hdl.handle.net/11449/177339
identifier_str_mv Ecological Indicators, v. 56, p. 60-69.
1470-160X
10.1016/j.ecolind.2015.03.026
2-s2.0-84928473982
2-s2.0-84928473982.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ecological Indicators
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 60-69
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129318194249728