Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0063 http://hdl.handle.net/11449/240049 |
Resumo: | Modified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility and bioactivity. Considering the relevance of osteoblastic cell response to mineralized tissue repair, human osteoblastic cells (Saos-2 cells overexpressing BMP-2) were exposed to mineral trioxide aggregate (MTA) (with calcium tungstate -CaWO4), MTA HP Repair, Bio-C Repair and Bio-C Pulpo. Cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR), and cell death, by flow cytometry. Gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX-2), and alkaline phosphatase (ALP) osteogenic markers were evaluated by real-time polymerase chain reaction (RT-qPCR). ALP activity and alizarin red staining (ARS) were used to detect mineralization nodule deposition. Bioactive cements presented no cytotoxic effect, and did not induce apoptosis at the higher dilution (1:12). MTA, Bio-C Repair and Bio-C Pulpo exhibited higher ALP activity than the control group (P< 0.05) after 7 days. MTA, MTA HP and Bio-C Pulpo affected the formation of mineralized nodules (p < 0.05). Exposure to all cement extracts for 1 day increased BMP-2 gene expression. RUNX-2 mRNA was greater in MTA, MTA HP and Bio-C Repair. MTA, MTA HP and Bio-C Pulpo increased the ALP mRNA expression, compared with BMP-2 unexposed cells (p<0.05). Calcium silicate cements showed osteogenic potential and biocompatibility in Saos-2 cells transfected BMP-2, and increased the mRNA expression of BMP-2, RUNX-2, and ALP osteogenic markers in the BMP-2 transfected system, thereby promoting a cellular response to undertake the mineralized tissue repair. |
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Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cellsBiocompatible MaterialsBone Morphogenetic Protein 2Cell ProliferationCell SurvivalEndodonticsModified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility and bioactivity. Considering the relevance of osteoblastic cell response to mineralized tissue repair, human osteoblastic cells (Saos-2 cells overexpressing BMP-2) were exposed to mineral trioxide aggregate (MTA) (with calcium tungstate -CaWO4), MTA HP Repair, Bio-C Repair and Bio-C Pulpo. Cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR), and cell death, by flow cytometry. Gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX-2), and alkaline phosphatase (ALP) osteogenic markers were evaluated by real-time polymerase chain reaction (RT-qPCR). ALP activity and alizarin red staining (ARS) were used to detect mineralization nodule deposition. Bioactive cements presented no cytotoxic effect, and did not induce apoptosis at the higher dilution (1:12). MTA, Bio-C Repair and Bio-C Pulpo exhibited higher ALP activity than the control group (P< 0.05) after 7 days. MTA, MTA HP and Bio-C Pulpo affected the formation of mineralized nodules (p < 0.05). Exposure to all cement extracts for 1 day increased BMP-2 gene expression. RUNX-2 mRNA was greater in MTA, MTA HP and Bio-C Repair. MTA, MTA HP and Bio-C Pulpo increased the ALP mRNA expression, compared with BMP-2 unexposed cells (p<0.05). Calcium silicate cements showed osteogenic potential and biocompatibility in Saos-2 cells transfected BMP-2, and increased the mRNA expression of BMP-2, RUNX-2, and ALP osteogenic markers in the BMP-2 transfected system, thereby promoting a cellular response to undertake the mineralized tissue repair.Universidade Estadual Paulista – Unesp Schoool of Dentistry Department of Restorative Dentistry, SPUniversidade Estadual Paulista – Unesp Schoool of Dentistry Department of Diagnosis and Surgery, SPUniversidade Estadual Paulista – Unesp Schoool of Dentistry Department of Restorative Dentistry, SPUniversidade Estadual Paulista – Unesp Schoool of Dentistry Department of Diagnosis and Surgery, SPUniversidade Estadual Paulista (UNESP)Rodrigues, Elisandra Márcia [UNESP]Viola, Kennia Scapin [UNESP]Maldonado, Laura Gonzalez [UNESP]Rossa-Junior, Carlos [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Tanomaru-Filho, Mario [UNESP]2023-03-01T19:59:10Z2023-03-01T19:59:10Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0063Brazilian Oral Research, v. 36.1807-31071806-8324http://hdl.handle.net/11449/24004910.1590/1807-3107bor-2022.vol36.00632-s2.0-85129966469Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-03-01T19:59:10Zoai:repositorio.unesp.br:11449/240049Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T19:59:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
title |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
spellingShingle |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells Rodrigues, Elisandra Márcia [UNESP] Biocompatible Materials Bone Morphogenetic Protein 2 Cell Proliferation Cell Survival Endodontics |
title_short |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
title_full |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
title_fullStr |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
title_full_unstemmed |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
title_sort |
Cytotoxicity and bioactive potential of new root repair materials for use with BMP-2 transfected human osteoblast cells |
author |
Rodrigues, Elisandra Márcia [UNESP] |
author_facet |
Rodrigues, Elisandra Márcia [UNESP] Viola, Kennia Scapin [UNESP] Maldonado, Laura Gonzalez [UNESP] Rossa-Junior, Carlos [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
author_role |
author |
author2 |
Viola, Kennia Scapin [UNESP] Maldonado, Laura Gonzalez [UNESP] Rossa-Junior, Carlos [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Rodrigues, Elisandra Márcia [UNESP] Viola, Kennia Scapin [UNESP] Maldonado, Laura Gonzalez [UNESP] Rossa-Junior, Carlos [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Tanomaru-Filho, Mario [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatible Materials Bone Morphogenetic Protein 2 Cell Proliferation Cell Survival Endodontics |
topic |
Biocompatible Materials Bone Morphogenetic Protein 2 Cell Proliferation Cell Survival Endodontics |
description |
Modified formulations of calcium silicate repair materials with additives have been developed to enhance handling, consistency, biocompatibility and bioactivity. Considering the relevance of osteoblastic cell response to mineralized tissue repair, human osteoblastic cells (Saos-2 cells overexpressing BMP-2) were exposed to mineral trioxide aggregate (MTA) (with calcium tungstate -CaWO4), MTA HP Repair, Bio-C Repair and Bio-C Pulpo. Cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR), and cell death, by flow cytometry. Gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX-2), and alkaline phosphatase (ALP) osteogenic markers were evaluated by real-time polymerase chain reaction (RT-qPCR). ALP activity and alizarin red staining (ARS) were used to detect mineralization nodule deposition. Bioactive cements presented no cytotoxic effect, and did not induce apoptosis at the higher dilution (1:12). MTA, Bio-C Repair and Bio-C Pulpo exhibited higher ALP activity than the control group (P< 0.05) after 7 days. MTA, MTA HP and Bio-C Pulpo affected the formation of mineralized nodules (p < 0.05). Exposure to all cement extracts for 1 day increased BMP-2 gene expression. RUNX-2 mRNA was greater in MTA, MTA HP and Bio-C Repair. MTA, MTA HP and Bio-C Pulpo increased the ALP mRNA expression, compared with BMP-2 unexposed cells (p<0.05). Calcium silicate cements showed osteogenic potential and biocompatibility in Saos-2 cells transfected BMP-2, and increased the mRNA expression of BMP-2, RUNX-2, and ALP osteogenic markers in the BMP-2 transfected system, thereby promoting a cellular response to undertake the mineralized tissue repair. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T19:59:10Z 2023-03-01T19:59:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0063 Brazilian Oral Research, v. 36. 1807-3107 1806-8324 http://hdl.handle.net/11449/240049 10.1590/1807-3107bor-2022.vol36.0063 2-s2.0-85129966469 |
url |
http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0063 http://hdl.handle.net/11449/240049 |
identifier_str_mv |
Brazilian Oral Research, v. 36. 1807-3107 1806-8324 10.1590/1807-3107bor-2022.vol36.0063 2-s2.0-85129966469 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046227335446528 |