Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs

Detalhes bibliográficos
Autor(a) principal: Ambrósio, DL [UNESP]
Data de Publicação: 2007
Outros Autores: Silva, MTA [UNESP], Cicarelli, RMB [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762007000100017
http://hdl.handle.net/11449/8021
Resumo: Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.
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spelling Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAsTrypanosoma cruziribonucleoproteinsmall nuclear RNPsmall nuclear RNAspliceosometrans-splicingSmall nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências BiológicasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Ambrósio, DL [UNESP]Silva, MTA [UNESP]Cicarelli, RMB [UNESP]2014-05-20T13:25:21Z2014-05-20T13:25:21Z2007-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-106application/pdfhttp://dx.doi.org/10.1590/S0074-02762007000100017Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 97-106, 2007.0074-0276http://hdl.handle.net/11449/802110.1590/S0074-02762007000100017S0074-02762007000100017S0074-02762007000100017.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-24T13:07:51Zoai:repositorio.unesp.br:11449/8021Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:14:17.101230Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
title Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
spellingShingle Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Ambrósio, DL [UNESP]
Trypanosoma cruzi
ribonucleoprotein
small nuclear RNP
small nuclear RNA
spliceosome
trans-splicing
title_short Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
title_full Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
title_fullStr Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
title_full_unstemmed Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
title_sort Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
author Ambrósio, DL [UNESP]
author_facet Ambrósio, DL [UNESP]
Silva, MTA [UNESP]
Cicarelli, RMB [UNESP]
author_role author
author2 Silva, MTA [UNESP]
Cicarelli, RMB [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ambrósio, DL [UNESP]
Silva, MTA [UNESP]
Cicarelli, RMB [UNESP]
dc.subject.por.fl_str_mv Trypanosoma cruzi
ribonucleoprotein
small nuclear RNP
small nuclear RNA
spliceosome
trans-splicing
topic Trypanosoma cruzi
ribonucleoprotein
small nuclear RNP
small nuclear RNA
spliceosome
trans-splicing
description Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-01
2014-05-20T13:25:21Z
2014-05-20T13:25:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762007000100017
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 97-106, 2007.
0074-0276
http://hdl.handle.net/11449/8021
10.1590/S0074-02762007000100017
S0074-02762007000100017
S0074-02762007000100017.pdf
url http://dx.doi.org/10.1590/S0074-02762007000100017
http://hdl.handle.net/11449/8021
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 97-106, 2007.
0074-0276
10.1590/S0074-02762007000100017
S0074-02762007000100017
S0074-02762007000100017.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 97-106
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129039421931520

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