Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/195995 |
Resumo: | Introduction: HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate and adaptive immune responses, and the expression of these molecules in virus infected cells has been associated with subversion of the immune response. Objective: In this study we performed a cross-sectional study, systematically comparing the expression of HLA-G and HLA-E in benign, premalignant and malignant laryngeal lesions, correlating with demographic and clinical variables and with the presence of high-risk and low-risk HPV types. Materials and methods: Laryngeal lesions were collected from 109 patients and stratified into 27 laryngeal papillomas, 17 dysplasias, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, 28 laryngeal carcinomas with metastasis along with their respective draining cervical lymph nodes, and 10 normal larynx specimens. The expression of HLA-G and HLA-E molecules was determined by immunohistochemistry. HPV DNA detection and typing was performed using generic and specific primers. Results: HLA nonclassical molecules showed a distinct distribution pattern, according to the larynx lesion grade. HLA-G expression increased in benign and premalignant lesions, and gradually decreased in invasive carcinomas and in respective draining cervical lymph nodes. Conversely, HLA-E expression increased as far as lesion grade increased, including increased molecule expression in the draining lymph nodes of malignant lesions. Only 17 (15.6%) patients were HPV DNA positive. Conclusions: Overexpression of HLA-E and underexpression of HLA-G appear to be good markers for malignant larynx lesion. |
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Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasivenessHLA-GHLA-ELaryngeal lesionsHPVBiomarkersIntroduction: HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate and adaptive immune responses, and the expression of these molecules in virus infected cells has been associated with subversion of the immune response. Objective: In this study we performed a cross-sectional study, systematically comparing the expression of HLA-G and HLA-E in benign, premalignant and malignant laryngeal lesions, correlating with demographic and clinical variables and with the presence of high-risk and low-risk HPV types. Materials and methods: Laryngeal lesions were collected from 109 patients and stratified into 27 laryngeal papillomas, 17 dysplasias, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, 28 laryngeal carcinomas with metastasis along with their respective draining cervical lymph nodes, and 10 normal larynx specimens. The expression of HLA-G and HLA-E molecules was determined by immunohistochemistry. HPV DNA detection and typing was performed using generic and specific primers. Results: HLA nonclassical molecules showed a distinct distribution pattern, according to the larynx lesion grade. HLA-G expression increased in benign and premalignant lesions, and gradually decreased in invasive carcinomas and in respective draining cervical lymph nodes. Conversely, HLA-E expression increased as far as lesion grade increased, including increased molecule expression in the draining lymph nodes of malignant lesions. Only 17 (15.6%) patients were HPV DNA positive. Conclusions: Overexpression of HLA-E and underexpression of HLA-G appear to be good markers for malignant larynx lesion.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP, Fac Pharmaceut Sci, Lab Cytol & Cell Biol, Sch Pharmaceut Sci,Dept Clin Anal, BR-14801902 Araraquara, SP, BrazilUniv Fed Rio Grande do Norte, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Natal, RN, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Div Clin Immunol, Dept Med, BR-05508 Sao Paulo, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Pathol, BR-05508 Sao Paulo, BrazilUniv Fed Mato Grosso, Dept Internal Med, Cuiaba, Mato Grosso, BrazilUNESP, Fac Pharmaceut Sci, Lab Cytol & Cell Biol, Sch Pharmaceut Sci,Dept Clin Anal, BR-14801902 Araraquara, SP, BrazilF HernandezUniversidade Estadual Paulista (Unesp)Univ Fed Rio Grande do NorteUniversidade de São Paulo (USP)Universidade Federal de Mato Grosso do Sul (UFMS)Silva, Tarsia G.Crispim, Janaina C. O.Miranda, Fabiana A.Hassumi, Marcela K.Mello, Julia M. Y. deSimoes, Renata T.Souto, FranciscoSoares, Edson G.Donadi, Eduardo A.Soares, Christiane P. [UNESP]2020-12-10T19:01:04Z2020-12-10T19:01:04Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1487-1497Histology And Histopathology. Murcia: F Hernandez, v. 26, n. 12, p. 1487-1497, 2011.0213-3911http://hdl.handle.net/11449/195995WOS:00029884310000117680252903736690000-0003-1740-7360Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology And Histopathologyinfo:eu-repo/semantics/openAccess2021-10-22T21:02:55Zoai:repositorio.unesp.br:11449/195995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:02:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
title |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
spellingShingle |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness Silva, Tarsia G. HLA-G HLA-E Laryngeal lesions HPV Biomarkers |
title_short |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
title_full |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
title_fullStr |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
title_full_unstemmed |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
title_sort |
Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness |
author |
Silva, Tarsia G. |
author_facet |
Silva, Tarsia G. Crispim, Janaina C. O. Miranda, Fabiana A. Hassumi, Marcela K. Mello, Julia M. Y. de Simoes, Renata T. Souto, Francisco Soares, Edson G. Donadi, Eduardo A. Soares, Christiane P. [UNESP] |
author_role |
author |
author2 |
Crispim, Janaina C. O. Miranda, Fabiana A. Hassumi, Marcela K. Mello, Julia M. Y. de Simoes, Renata T. Souto, Francisco Soares, Edson G. Donadi, Eduardo A. Soares, Christiane P. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Fed Rio Grande do Norte Universidade de São Paulo (USP) Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Silva, Tarsia G. Crispim, Janaina C. O. Miranda, Fabiana A. Hassumi, Marcela K. Mello, Julia M. Y. de Simoes, Renata T. Souto, Francisco Soares, Edson G. Donadi, Eduardo A. Soares, Christiane P. [UNESP] |
dc.subject.por.fl_str_mv |
HLA-G HLA-E Laryngeal lesions HPV Biomarkers |
topic |
HLA-G HLA-E Laryngeal lesions HPV Biomarkers |
description |
Introduction: HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate and adaptive immune responses, and the expression of these molecules in virus infected cells has been associated with subversion of the immune response. Objective: In this study we performed a cross-sectional study, systematically comparing the expression of HLA-G and HLA-E in benign, premalignant and malignant laryngeal lesions, correlating with demographic and clinical variables and with the presence of high-risk and low-risk HPV types. Materials and methods: Laryngeal lesions were collected from 109 patients and stratified into 27 laryngeal papillomas, 17 dysplasias, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, 28 laryngeal carcinomas with metastasis along with their respective draining cervical lymph nodes, and 10 normal larynx specimens. The expression of HLA-G and HLA-E molecules was determined by immunohistochemistry. HPV DNA detection and typing was performed using generic and specific primers. Results: HLA nonclassical molecules showed a distinct distribution pattern, according to the larynx lesion grade. HLA-G expression increased in benign and premalignant lesions, and gradually decreased in invasive carcinomas and in respective draining cervical lymph nodes. Conversely, HLA-E expression increased as far as lesion grade increased, including increased molecule expression in the draining lymph nodes of malignant lesions. Only 17 (15.6%) patients were HPV DNA positive. Conclusions: Overexpression of HLA-E and underexpression of HLA-G appear to be good markers for malignant larynx lesion. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 2020-12-10T19:01:04Z 2020-12-10T19:01:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 26, n. 12, p. 1487-1497, 2011. 0213-3911 http://hdl.handle.net/11449/195995 WOS:000298843100001 1768025290373669 0000-0003-1740-7360 |
identifier_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 26, n. 12, p. 1487-1497, 2011. 0213-3911 WOS:000298843100001 1768025290373669 0000-0003-1740-7360 |
url |
http://hdl.handle.net/11449/195995 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology And Histopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1487-1497 |
dc.publisher.none.fl_str_mv |
F Hernandez |
publisher.none.fl_str_mv |
F Hernandez |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965708777422848 |