Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples

Detalhes bibliográficos
Autor(a) principal: Lamarca, Rafaela Silva [UNESP]
Data de Publicação: 2020
Outros Autores: Franco, Douglas Faza [UNESP], Nalin, Marcelo [UNESP], De Lima Gomes, Paulo Clairmont Feitosa [UNESP], Messaddeq, Younès [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1021/acs.analchem.0c02355
http://hdl.handle.net/11449/208146
Resumo: Emerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater.
id UNSP_03e8311d141b76d6472b9b9879473366
oai_identifier_str oai:repositorio.unesp.br:11449/208146
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater SamplesEmerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater.Center for Optics Photonics and Laser (COPL) Université LavalNational Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry São Paulo State University (UNESP)Institute of Chemistry São Paulo State University (UNESP)National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry São Paulo State University (UNESP)Institute of Chemistry São Paulo State University (UNESP)Université LavalUniversidade Estadual Paulista (Unesp)Lamarca, Rafaela Silva [UNESP]Franco, Douglas Faza [UNESP]Nalin, Marcelo [UNESP]De Lima Gomes, Paulo Clairmont Feitosa [UNESP]Messaddeq, Younès [UNESP]2021-06-25T11:07:13Z2021-06-25T11:07:13Z2020-11-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14415-14422http://dx.doi.org/10.1021/acs.analchem.0c02355Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020.1520-68820003-2700http://hdl.handle.net/11449/20814610.1021/acs.analchem.0c023552-s2.0-85096096769Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytical Chemistryinfo:eu-repo/semantics/openAccess2021-10-23T18:56:38Zoai:repositorio.unesp.br:11449/208146Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:23:14.666348Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
title Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
spellingShingle Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
Lamarca, Rafaela Silva [UNESP]
title_short Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
title_full Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
title_fullStr Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
title_full_unstemmed Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
title_sort Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
author Lamarca, Rafaela Silva [UNESP]
author_facet Lamarca, Rafaela Silva [UNESP]
Franco, Douglas Faza [UNESP]
Nalin, Marcelo [UNESP]
De Lima Gomes, Paulo Clairmont Feitosa [UNESP]
Messaddeq, Younès [UNESP]
author_role author
author2 Franco, Douglas Faza [UNESP]
Nalin, Marcelo [UNESP]
De Lima Gomes, Paulo Clairmont Feitosa [UNESP]
Messaddeq, Younès [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Université Laval
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Lamarca, Rafaela Silva [UNESP]
Franco, Douglas Faza [UNESP]
Nalin, Marcelo [UNESP]
De Lima Gomes, Paulo Clairmont Feitosa [UNESP]
Messaddeq, Younès [UNESP]
description Emerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-03
2021-06-25T11:07:13Z
2021-06-25T11:07:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1021/acs.analchem.0c02355
Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020.
1520-6882
0003-2700
http://hdl.handle.net/11449/208146
10.1021/acs.analchem.0c02355
2-s2.0-85096096769
url http://dx.doi.org/10.1021/acs.analchem.0c02355
http://hdl.handle.net/11449/208146
identifier_str_mv Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020.
1520-6882
0003-2700
10.1021/acs.analchem.0c02355
2-s2.0-85096096769
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytical Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14415-14422
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129061285789696