Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.analchem.0c02355 http://hdl.handle.net/11449/208146 |
Resumo: | Emerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater. |
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Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater SamplesEmerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater.Center for Optics Photonics and Laser (COPL) Université LavalNational Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry São Paulo State University (UNESP)Institute of Chemistry São Paulo State University (UNESP)National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Institute of Chemistry São Paulo State University (UNESP)Institute of Chemistry São Paulo State University (UNESP)Université LavalUniversidade Estadual Paulista (Unesp)Lamarca, Rafaela Silva [UNESP]Franco, Douglas Faza [UNESP]Nalin, Marcelo [UNESP]De Lima Gomes, Paulo Clairmont Feitosa [UNESP]Messaddeq, Younès [UNESP]2021-06-25T11:07:13Z2021-06-25T11:07:13Z2020-11-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14415-14422http://dx.doi.org/10.1021/acs.analchem.0c02355Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020.1520-68820003-2700http://hdl.handle.net/11449/20814610.1021/acs.analchem.0c023552-s2.0-85096096769Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytical Chemistryinfo:eu-repo/semantics/openAccess2021-10-23T18:56:38Zoai:repositorio.unesp.br:11449/208146Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:23:14.666348Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
title |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
spellingShingle |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples Lamarca, Rafaela Silva [UNESP] |
title_short |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
title_full |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
title_fullStr |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
title_full_unstemmed |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
title_sort |
Label-Free Ultrasensitive and Environment-Friendly Immunosensor Based on a Silica Optical Fiber for the Determination of Ciprofloxacin in Wastewater Samples |
author |
Lamarca, Rafaela Silva [UNESP] |
author_facet |
Lamarca, Rafaela Silva [UNESP] Franco, Douglas Faza [UNESP] Nalin, Marcelo [UNESP] De Lima Gomes, Paulo Clairmont Feitosa [UNESP] Messaddeq, Younès [UNESP] |
author_role |
author |
author2 |
Franco, Douglas Faza [UNESP] Nalin, Marcelo [UNESP] De Lima Gomes, Paulo Clairmont Feitosa [UNESP] Messaddeq, Younès [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Université Laval Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lamarca, Rafaela Silva [UNESP] Franco, Douglas Faza [UNESP] Nalin, Marcelo [UNESP] De Lima Gomes, Paulo Clairmont Feitosa [UNESP] Messaddeq, Younès [UNESP] |
description |
Emerging contaminants, including pharmaceutical compounds, are receiving research attention as a result of their widespread presence in effluents and wastewater treatment plants (WWTPs). The antibiotic ciprofloxacin (CIP) is extensively employed to treat infections in animal and human medicine. Both CIP and its metabolites are common contaminants found in WWTPs. In this study, a label-free ultrasensitive U-bent optical fiber-based immunosensor for the determination of CIP in wastewater samples was developed using the properties of the conducting polymer polyaniline (PANI). The anti-CIP immunoglobulin G (IgG) was deposited on a silica optical fiber surface previously functionalized with PANI. Scanning electron microscopy and micro-Raman spectroscopy were used to investigate the surface of the immunosensor. The analysis of CIP in wastewater was performed without the use of an organic solvent or sample preparation steps, with only the sample dilution in saline buffer (pH = 7.4). The linear range for CIP was from 0.01 to 10,000 ng L-1. The detection limit was 3.30 × 10-3 ng L-1 and the quantification limit was 0.01 ng L-1. The immunosensor provided a high average recovery of 91% after spiking wastewater samples with CIP at a concentration of 9,100 ng L-1. The method was applied in triplicate to wastewater samples from Quebec (Canada), obtaining concentrations of 549 and 267 ng L-1. A comparison with a reference method showed no significant difference (t-test at 95% confidence). The new technique developed is selective, allowing a quantitative analysis of CIP in wastewater. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-03 2021-06-25T11:07:13Z 2021-06-25T11:07:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.analchem.0c02355 Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020. 1520-6882 0003-2700 http://hdl.handle.net/11449/208146 10.1021/acs.analchem.0c02355 2-s2.0-85096096769 |
url |
http://dx.doi.org/10.1021/acs.analchem.0c02355 http://hdl.handle.net/11449/208146 |
identifier_str_mv |
Analytical Chemistry, v. 92, n. 21, p. 14415-14422, 2020. 1520-6882 0003-2700 10.1021/acs.analchem.0c02355 2-s2.0-85096096769 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytical Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14415-14422 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129061285789696 |