Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2020.07.051 http://hdl.handle.net/11449/200846 |
Resumo: | Streptococcus agalactiae is considered the main bacterial pathogen in cultured Nile tilapia. Formaldehyde-inactivated vaccines are the most accepted method for prevention and control of the disease. However, alternative inactivation methods for S. agalactiae vaccines have not been fully explored. Recently, we developed a hydrogen peroxide-inactivated vaccine against S. agalactiae with moderate efficacy, with the possibility to improve vaccine efficacy by adding adjuvants. The current study compared the efficacy of aluminum hydroxide and Freund's incomplete adjuvant (FIA) incorporated into a novel hydrogen peroxide-inactivated intraperitoneal vaccine against S. agalactiae for Nile tilapia fingerlings. The relative percentage survival (RPS) for aluminum hydroxide-adjuvanted vaccine (59.3%), and FIA-adjuvanted vaccine (77.8%) were higher than the vaccine without adjuvant (40.7%). In addition, fish immunized with aluminum hydroxide-adjuvanted vaccine had significantly higher levels of specific antibodies than control fish at 4 weeks post vaccination (wpv). Blood lymphocytes counts showed a decrease in vaccinated groups when compared to control fish, suggesting white cells migration to the tissues where antigen presentation is ongoing. Fish that received FIA-adjuvanted vaccine exhibited persistence of adjuvant deposits on intraperitoneal surfaces for at least 4 wpv that may be related to its superior performance compared to aluminum hydroxide adjuvanted vaccine, which did not evidence any type of deposit at any sampling times. The results observed in this study demonstrate that hydrogen peroxide-inactivated vaccine administered with either aluminum hydroxide or FIA induce optimal levels of protection, with a superior performance for FIA vaccine, which could be a good alternative to conventional formaldehyde-inactivated vaccines against S. agalactiae, due to its shorter manufacture time, and less toxicity. |
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Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlingsAluminum hydroxideFreund incomplete adjuvantImmune responseOreochromis niloticusVaccine efficacyStreptococcus agalactiae is considered the main bacterial pathogen in cultured Nile tilapia. Formaldehyde-inactivated vaccines are the most accepted method for prevention and control of the disease. However, alternative inactivation methods for S. agalactiae vaccines have not been fully explored. Recently, we developed a hydrogen peroxide-inactivated vaccine against S. agalactiae with moderate efficacy, with the possibility to improve vaccine efficacy by adding adjuvants. The current study compared the efficacy of aluminum hydroxide and Freund's incomplete adjuvant (FIA) incorporated into a novel hydrogen peroxide-inactivated intraperitoneal vaccine against S. agalactiae for Nile tilapia fingerlings. The relative percentage survival (RPS) for aluminum hydroxide-adjuvanted vaccine (59.3%), and FIA-adjuvanted vaccine (77.8%) were higher than the vaccine without adjuvant (40.7%). In addition, fish immunized with aluminum hydroxide-adjuvanted vaccine had significantly higher levels of specific antibodies than control fish at 4 weeks post vaccination (wpv). Blood lymphocytes counts showed a decrease in vaccinated groups when compared to control fish, suggesting white cells migration to the tissues where antigen presentation is ongoing. Fish that received FIA-adjuvanted vaccine exhibited persistence of adjuvant deposits on intraperitoneal surfaces for at least 4 wpv that may be related to its superior performance compared to aluminum hydroxide adjuvanted vaccine, which did not evidence any type of deposit at any sampling times. The results observed in this study demonstrate that hydrogen peroxide-inactivated vaccine administered with either aluminum hydroxide or FIA induce optimal levels of protection, with a superior performance for FIA vaccine, which could be a good alternative to conventional formaldehyde-inactivated vaccines against S. agalactiae, due to its shorter manufacture time, and less toxicity.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05Aquatic Pathobiology Laboratory Sea Institute of Peru (IMARPE), Esquina Gamarra y GeneralValle s/n, ChucuitoDepartment of Veterinary Pathology School of Agricultural and Veterinary Sciences (FCAV) Unesp, Via Prof. Paulo Donato Castellane, km 05Aquaculture Center of Unesp (Caunesp) Sao Paulo State University (Unesp), Via Prof. Paulo Donato Castellane, km 05Department of Veterinary Pathology School of Agricultural and Veterinary Sciences (FCAV) Unesp, Via Prof. Paulo Donato Castellane, km 05CNPq: 141835/2018-4FAPESP: 2018/06137-1FAPESP: 2019/02339-1Universidade Estadual Paulista (Unesp)Sea Institute of Peru (IMARPE)Ramos-Espinoza, Fernando Carlos [UNESP]Cueva-Quiroz, Victor Alexander [UNESP]Yunis-Aguinaga, Jefferson [UNESP]Alvarez-Rubio, Norquis Caled [UNESP]Paganoti de Mello, Nicoli [UNESP]Engrácia de Moraes, Julieta Rodini [UNESP]2020-12-12T02:17:39Z2020-12-12T02:17:39Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article350-358http://dx.doi.org/10.1016/j.fsi.2020.07.051Fish and Shellfish Immunology, v. 105, p. 350-358.1095-99471050-4648http://hdl.handle.net/11449/20084610.1016/j.fsi.2020.07.0512-s2.0-85088962521Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/200846Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:25:44.221084Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
title |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
spellingShingle |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings Ramos-Espinoza, Fernando Carlos [UNESP] Aluminum hydroxide Freund incomplete adjuvant Immune response Oreochromis niloticus Vaccine efficacy |
title_short |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
title_full |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
title_fullStr |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
title_full_unstemmed |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
title_sort |
Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings |
author |
Ramos-Espinoza, Fernando Carlos [UNESP] |
author_facet |
Ramos-Espinoza, Fernando Carlos [UNESP] Cueva-Quiroz, Victor Alexander [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Alvarez-Rubio, Norquis Caled [UNESP] Paganoti de Mello, Nicoli [UNESP] Engrácia de Moraes, Julieta Rodini [UNESP] |
author_role |
author |
author2 |
Cueva-Quiroz, Victor Alexander [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Alvarez-Rubio, Norquis Caled [UNESP] Paganoti de Mello, Nicoli [UNESP] Engrácia de Moraes, Julieta Rodini [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Sea Institute of Peru (IMARPE) |
dc.contributor.author.fl_str_mv |
Ramos-Espinoza, Fernando Carlos [UNESP] Cueva-Quiroz, Victor Alexander [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Alvarez-Rubio, Norquis Caled [UNESP] Paganoti de Mello, Nicoli [UNESP] Engrácia de Moraes, Julieta Rodini [UNESP] |
dc.subject.por.fl_str_mv |
Aluminum hydroxide Freund incomplete adjuvant Immune response Oreochromis niloticus Vaccine efficacy |
topic |
Aluminum hydroxide Freund incomplete adjuvant Immune response Oreochromis niloticus Vaccine efficacy |
description |
Streptococcus agalactiae is considered the main bacterial pathogen in cultured Nile tilapia. Formaldehyde-inactivated vaccines are the most accepted method for prevention and control of the disease. However, alternative inactivation methods for S. agalactiae vaccines have not been fully explored. Recently, we developed a hydrogen peroxide-inactivated vaccine against S. agalactiae with moderate efficacy, with the possibility to improve vaccine efficacy by adding adjuvants. The current study compared the efficacy of aluminum hydroxide and Freund's incomplete adjuvant (FIA) incorporated into a novel hydrogen peroxide-inactivated intraperitoneal vaccine against S. agalactiae for Nile tilapia fingerlings. The relative percentage survival (RPS) for aluminum hydroxide-adjuvanted vaccine (59.3%), and FIA-adjuvanted vaccine (77.8%) were higher than the vaccine without adjuvant (40.7%). In addition, fish immunized with aluminum hydroxide-adjuvanted vaccine had significantly higher levels of specific antibodies than control fish at 4 weeks post vaccination (wpv). Blood lymphocytes counts showed a decrease in vaccinated groups when compared to control fish, suggesting white cells migration to the tissues where antigen presentation is ongoing. Fish that received FIA-adjuvanted vaccine exhibited persistence of adjuvant deposits on intraperitoneal surfaces for at least 4 wpv that may be related to its superior performance compared to aluminum hydroxide adjuvanted vaccine, which did not evidence any type of deposit at any sampling times. The results observed in this study demonstrate that hydrogen peroxide-inactivated vaccine administered with either aluminum hydroxide or FIA induce optimal levels of protection, with a superior performance for FIA vaccine, which could be a good alternative to conventional formaldehyde-inactivated vaccines against S. agalactiae, due to its shorter manufacture time, and less toxicity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:17:39Z 2020-12-12T02:17:39Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2020.07.051 Fish and Shellfish Immunology, v. 105, p. 350-358. 1095-9947 1050-4648 http://hdl.handle.net/11449/200846 10.1016/j.fsi.2020.07.051 2-s2.0-85088962521 |
url |
http://dx.doi.org/10.1016/j.fsi.2020.07.051 http://hdl.handle.net/11449/200846 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 105, p. 350-358. 1095-9947 1050-4648 10.1016/j.fsi.2020.07.051 2-s2.0-85088962521 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
350-358 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129200403513344 |