The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2164-10-330 http://hdl.handle.net/11449/42363 |
Resumo: | Background: For three decades the Mutator system was thought to be exclusive of plants, until the first homolog representatives were characterized in fungi and in early-diverging amoebas earlier in this decade.Results: Here, we describe and characterize four families of Mutator-like elements in a new eukaryotic group, the Parabasalids. These Trichomonas vaginalis Mutator-like elements, or TvMULEs, are active in T. vaginalis and patchily distributed among 12 trichomonad species and isolates. Despite their relatively distinctive amino acid composition, the inclusion of the repeats TvMULE1, TvMULE2, TvMULE3 and TvMULE4 into the Mutator superfamily is justified by sequence, structural and phylogenetic analyses. In addition, we identified three new TvMULE-related sequences in the genome sequence of Candida albicans. While TvMULE1 is a member of the MuDR clade, predominantly from plants, the other three TvMULEs, together with the C. albicans elements, represent a new and quite distinct Mutator lineage, which we named TvCaMULEs. The finding of TvMULE1 sequence inserted into other putative repeat suggests the occurrence a novel TE family not yet described.Conclusion: These findings expand the taxonomic distribution and the range of functional motif of MULEs among eukaryotes. The characterization of the dynamics of TvMULEs and other transposons in this organism is of particular interest because it is atypical for an asexual species to have such an extreme level of TE activity; this genetic landscape makes an interesting case study for causes and consequences of such activity. Finally, the extreme repetitiveness of the T. vaginalis genome and the remarkable degree of sequence identity within its repeat families highlights this species as an ideal system to characterize new transposable elements. |
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Repositório Institucional da UNESP |
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spelling |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elementsBackground: For three decades the Mutator system was thought to be exclusive of plants, until the first homolog representatives were characterized in fungi and in early-diverging amoebas earlier in this decade.Results: Here, we describe and characterize four families of Mutator-like elements in a new eukaryotic group, the Parabasalids. These Trichomonas vaginalis Mutator-like elements, or TvMULEs, are active in T. vaginalis and patchily distributed among 12 trichomonad species and isolates. Despite their relatively distinctive amino acid composition, the inclusion of the repeats TvMULE1, TvMULE2, TvMULE3 and TvMULE4 into the Mutator superfamily is justified by sequence, structural and phylogenetic analyses. In addition, we identified three new TvMULE-related sequences in the genome sequence of Candida albicans. While TvMULE1 is a member of the MuDR clade, predominantly from plants, the other three TvMULEs, together with the C. albicans elements, represent a new and quite distinct Mutator lineage, which we named TvCaMULEs. The finding of TvMULE1 sequence inserted into other putative repeat suggests the occurrence a novel TE family not yet described.Conclusion: These findings expand the taxonomic distribution and the range of functional motif of MULEs among eukaryotes. The characterization of the dynamics of TvMULEs and other transposons in this organism is of particular interest because it is atypical for an asexual species to have such an extreme level of TE activity; this genetic landscape makes an interesting case study for causes and consequences of such activity. Finally, the extreme repetitiveness of the T. vaginalis genome and the remarkable degree of sequence identity within its repeat families highlights this species as an ideal system to characterize new transposable elements.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State Univ, UNESP, Dept Biol, BR-15054000 São Paulo, BrazilUniv Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21201 USAUniv Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USAUSU Santa Ursula Univ, Inst Biol & Environm Sci, BR-22231010 Rio de Janeiro, BrazilUniv Estadual Campinas, UNICAMP, Dept Genet & Evolut, BR-13083970 Campinas, SP, BrazilSão Paulo State Univ, UNESP, Dept Biol, BR-15054000 São Paulo, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)University of MarylandUSU Santa Ursula UnivUniversidade Estadual de Campinas (UNICAMP)Lopes, Fabricio R. [UNESP]Silva, Joana C.Benchimol, MarleneCosta, Gustavo G. L.Pereira, Goncalo A. G.Carareto, Claudia M. A. [UNESP]2014-05-20T15:33:56Z2014-05-20T15:33:56Z2009-07-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1186/1471-2164-10-330Bmc Genomics. London: Biomed Central Ltd., v. 10, p. 16, 2009.1471-2164http://hdl.handle.net/11449/4236310.1186/1471-2164-10-330WOS:000269508100003WOS000269508100003.pdf34257729983192160000-0002-0298-1354Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Genomics3.7302,110info:eu-repo/semantics/openAccess2023-11-23T06:17:02Zoai:repositorio.unesp.br:11449/42363Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:17:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
title |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
spellingShingle |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements Lopes, Fabricio R. [UNESP] |
title_short |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
title_full |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
title_fullStr |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
title_full_unstemmed |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
title_sort |
The protist Trichomonas vaginalis harbors multiple lineages of transcriptionally active Mutator-like elements |
author |
Lopes, Fabricio R. [UNESP] |
author_facet |
Lopes, Fabricio R. [UNESP] Silva, Joana C. Benchimol, Marlene Costa, Gustavo G. L. Pereira, Goncalo A. G. Carareto, Claudia M. A. [UNESP] |
author_role |
author |
author2 |
Silva, Joana C. Benchimol, Marlene Costa, Gustavo G. L. Pereira, Goncalo A. G. Carareto, Claudia M. A. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Maryland USU Santa Ursula Univ Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Lopes, Fabricio R. [UNESP] Silva, Joana C. Benchimol, Marlene Costa, Gustavo G. L. Pereira, Goncalo A. G. Carareto, Claudia M. A. [UNESP] |
description |
Background: For three decades the Mutator system was thought to be exclusive of plants, until the first homolog representatives were characterized in fungi and in early-diverging amoebas earlier in this decade.Results: Here, we describe and characterize four families of Mutator-like elements in a new eukaryotic group, the Parabasalids. These Trichomonas vaginalis Mutator-like elements, or TvMULEs, are active in T. vaginalis and patchily distributed among 12 trichomonad species and isolates. Despite their relatively distinctive amino acid composition, the inclusion of the repeats TvMULE1, TvMULE2, TvMULE3 and TvMULE4 into the Mutator superfamily is justified by sequence, structural and phylogenetic analyses. In addition, we identified three new TvMULE-related sequences in the genome sequence of Candida albicans. While TvMULE1 is a member of the MuDR clade, predominantly from plants, the other three TvMULEs, together with the C. albicans elements, represent a new and quite distinct Mutator lineage, which we named TvCaMULEs. The finding of TvMULE1 sequence inserted into other putative repeat suggests the occurrence a novel TE family not yet described.Conclusion: These findings expand the taxonomic distribution and the range of functional motif of MULEs among eukaryotes. The characterization of the dynamics of TvMULEs and other transposons in this organism is of particular interest because it is atypical for an asexual species to have such an extreme level of TE activity; this genetic landscape makes an interesting case study for causes and consequences of such activity. Finally, the extreme repetitiveness of the T. vaginalis genome and the remarkable degree of sequence identity within its repeat families highlights this species as an ideal system to characterize new transposable elements. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-21 2014-05-20T15:33:56Z 2014-05-20T15:33:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2164-10-330 Bmc Genomics. London: Biomed Central Ltd., v. 10, p. 16, 2009. 1471-2164 http://hdl.handle.net/11449/42363 10.1186/1471-2164-10-330 WOS:000269508100003 WOS000269508100003.pdf 3425772998319216 0000-0002-0298-1354 |
url |
http://dx.doi.org/10.1186/1471-2164-10-330 http://hdl.handle.net/11449/42363 |
identifier_str_mv |
Bmc Genomics. London: Biomed Central Ltd., v. 10, p. 16, 2009. 1471-2164 10.1186/1471-2164-10-330 WOS:000269508100003 WOS000269508100003.pdf 3425772998319216 0000-0002-0298-1354 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Genomics 3.730 2,110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965046277668864 |