The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000515234 http://hdl.handle.net/11449/207955 |
Resumo: | This study evaluated the effect of experimental solutions containing plant extracts on bacterial species and enamel caries prevention. Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for 5 days (3 days under anaerobiosis and 2 days under aerobiosis) at 37°C. From the 2nd day, the following treatments were applied (1 × 60 s/day): Vochysia tucanorum (10 mg/mL); Myrcia bella (5 mg/mL); Matricaria chamomilla (80 mg/mL); Malva sylvestris, fluoride, and xylitol (Malvatricin Plus®); 0.12% chlorhexidine (CHX, PerioGard®); and PBS (negative control). The medium pH was measured. Quantitative polymerase chain reaction was performed for the detection of Streptococcus mutans and Lactobacillus spp. Enamel demineralization was measured by spectral-domain optical coherence tomography. The data were compared by means of the Kruskal-Wallis/Dunn, two-way ANOVA/Bonferroni, and ANOVA/Tukey tests (p < 0.05). The pH decreased after sucrose exposure; only CHX reestablished pH >5.5 by the last day. CHX also eliminated Lactobacillusspp., but the other treatments did not differ significantly from PBS. Malvatricin Plus® and CHX eliminated S. mutans, but the other treatments did not differ from PBS. Similar results were seen concerning the reduction of lesion depth and reflectivity. The experimental natural-extract solutions were ineffective against cariogenic bacteria and in preventing the development of enamel caries. |
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The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries DevelopmentAntimicrobial agentsDental cariesDental hygieneOral biofilmThis study evaluated the effect of experimental solutions containing plant extracts on bacterial species and enamel caries prevention. Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for 5 days (3 days under anaerobiosis and 2 days under aerobiosis) at 37°C. From the 2nd day, the following treatments were applied (1 × 60 s/day): Vochysia tucanorum (10 mg/mL); Myrcia bella (5 mg/mL); Matricaria chamomilla (80 mg/mL); Malva sylvestris, fluoride, and xylitol (Malvatricin Plus®); 0.12% chlorhexidine (CHX, PerioGard®); and PBS (negative control). The medium pH was measured. Quantitative polymerase chain reaction was performed for the detection of Streptococcus mutans and Lactobacillus spp. Enamel demineralization was measured by spectral-domain optical coherence tomography. The data were compared by means of the Kruskal-Wallis/Dunn, two-way ANOVA/Bonferroni, and ANOVA/Tukey tests (p < 0.05). The pH decreased after sucrose exposure; only CHX reestablished pH >5.5 by the last day. CHX also eliminated Lactobacillusspp., but the other treatments did not differ significantly from PBS. Malvatricin Plus® and CHX eliminated S. mutans, but the other treatments did not differ from PBS. Similar results were seen concerning the reduction of lesion depth and reflectivity. The experimental natural-extract solutions were ineffective against cariogenic bacteria and in preventing the development of enamel caries.Department of Biological Sciences Bauru School of Dentistry University of São PauloDepartment of Biological Sciences School of Science São Paulo State University (UNESP)Department of Cariology Endodontology and Periodontology University of LeipzigDivision of Oral Microbiology and Immunology Department of Operative and Preventive Dentistry and Periodontology RWTH Aachen University HospitalDepartment of Restorative Preventive and Pediatric Dentistry University of BernDepartment of Biological Sciences School of Science São Paulo State University (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)University of LeipzigRWTH Aachen University HospitalUniversity of BernBraga, Aline SilvaDe Melo, Fernanda Pereira De Souza Rosa [UNESP]Saldanha, Luiz Leonardo [UNESP]Dokkedal, Anne Lígia [UNESP]Meissner, TobiasBemmann, MaximilianSchulz-Kornas, EllenHaak, RainerAbdelbary, Mohamed Mostafa HefnyConrads, GeorgMagalhães, Ana CarolinaEsteves-Oliveira, Marcella2021-06-25T11:03:49Z2021-06-25T11:03:49Z2021-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article193-204http://dx.doi.org/10.1159/000515234Caries Research, v. 55, n. 3, p. 193-204, 2021.1421-976X0008-6568http://hdl.handle.net/11449/20795510.1159/0005152342-s2.0-85107712935Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCaries Researchinfo:eu-repo/semantics/openAccess2024-04-23T15:23:39Zoai:repositorio.unesp.br:11449/207955Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
title |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
spellingShingle |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development Braga, Aline Silva Antimicrobial agents Dental caries Dental hygiene Oral biofilm |
title_short |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
title_full |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
title_fullStr |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
title_full_unstemmed |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
title_sort |
The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bella Cambess., Matricaria chamomilla L. And Malva sylvestris L. And Cariogenic Bacterial Species and Enamel Caries Development |
author |
Braga, Aline Silva |
author_facet |
Braga, Aline Silva De Melo, Fernanda Pereira De Souza Rosa [UNESP] Saldanha, Luiz Leonardo [UNESP] Dokkedal, Anne Lígia [UNESP] Meissner, Tobias Bemmann, Maximilian Schulz-Kornas, Ellen Haak, Rainer Abdelbary, Mohamed Mostafa Hefny Conrads, Georg Magalhães, Ana Carolina Esteves-Oliveira, Marcella |
author_role |
author |
author2 |
De Melo, Fernanda Pereira De Souza Rosa [UNESP] Saldanha, Luiz Leonardo [UNESP] Dokkedal, Anne Lígia [UNESP] Meissner, Tobias Bemmann, Maximilian Schulz-Kornas, Ellen Haak, Rainer Abdelbary, Mohamed Mostafa Hefny Conrads, Georg Magalhães, Ana Carolina Esteves-Oliveira, Marcella |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) University of Leipzig RWTH Aachen University Hospital University of Bern |
dc.contributor.author.fl_str_mv |
Braga, Aline Silva De Melo, Fernanda Pereira De Souza Rosa [UNESP] Saldanha, Luiz Leonardo [UNESP] Dokkedal, Anne Lígia [UNESP] Meissner, Tobias Bemmann, Maximilian Schulz-Kornas, Ellen Haak, Rainer Abdelbary, Mohamed Mostafa Hefny Conrads, Georg Magalhães, Ana Carolina Esteves-Oliveira, Marcella |
dc.subject.por.fl_str_mv |
Antimicrobial agents Dental caries Dental hygiene Oral biofilm |
topic |
Antimicrobial agents Dental caries Dental hygiene Oral biofilm |
description |
This study evaluated the effect of experimental solutions containing plant extracts on bacterial species and enamel caries prevention. Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for 5 days (3 days under anaerobiosis and 2 days under aerobiosis) at 37°C. From the 2nd day, the following treatments were applied (1 × 60 s/day): Vochysia tucanorum (10 mg/mL); Myrcia bella (5 mg/mL); Matricaria chamomilla (80 mg/mL); Malva sylvestris, fluoride, and xylitol (Malvatricin Plus®); 0.12% chlorhexidine (CHX, PerioGard®); and PBS (negative control). The medium pH was measured. Quantitative polymerase chain reaction was performed for the detection of Streptococcus mutans and Lactobacillus spp. Enamel demineralization was measured by spectral-domain optical coherence tomography. The data were compared by means of the Kruskal-Wallis/Dunn, two-way ANOVA/Bonferroni, and ANOVA/Tukey tests (p < 0.05). The pH decreased after sucrose exposure; only CHX reestablished pH >5.5 by the last day. CHX also eliminated Lactobacillusspp., but the other treatments did not differ significantly from PBS. Malvatricin Plus® and CHX eliminated S. mutans, but the other treatments did not differ from PBS. Similar results were seen concerning the reduction of lesion depth and reflectivity. The experimental natural-extract solutions were ineffective against cariogenic bacteria and in preventing the development of enamel caries. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:03:49Z 2021-06-25T11:03:49Z 2021-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000515234 Caries Research, v. 55, n. 3, p. 193-204, 2021. 1421-976X 0008-6568 http://hdl.handle.net/11449/207955 10.1159/000515234 2-s2.0-85107712935 |
url |
http://dx.doi.org/10.1159/000515234 http://hdl.handle.net/11449/207955 |
identifier_str_mv |
Caries Research, v. 55, n. 3, p. 193-204, 2021. 1421-976X 0008-6568 10.1159/000515234 2-s2.0-85107712935 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Caries Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
193-204 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046810176978944 |