Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0270-2016 http://hdl.handle.net/11449/162158 |
Resumo: | Introduction: Road-killed wild animals host zoonotic pathogens such as Toxoplasma gondii, offering a new opportunity for the epidemiological study of these infectious organisms. Methods: This investigation aimed to determine the presence of T. gondii and other apicomplexan parasites in tissue samples of 64 road-killed wild animals, using polymerase chain reaction (PCR). Positive samples were then typed by PCR-restriction fragment length polymorphism (RFLP) using 7 markers: SAG1, 5'-3' SAG2, SAG3, BTUB, c29-6, PK1, and Apico. PCR-RFLP targeting 18S ribosomal RNA (rRNA) genes was also performed on all samples to detect other apicomplexan parasites. Results: T. gondii DNA was detected in 16 tissue samples from 8 individual animals, as follows: 1 Cerdocyon thous (crab-eating fox), 1 Didelphis albiventris (white-eared opossum), 1 Lutreolina crassicaudata (lutrine opossum), 2 Myrmecophaga tridactyla (giant anteater), 1 Procyon cancrivorus (crab-eating raccoon), and 2 Sphiggurus spinosus (Paraguay hairy dwarf porcupine). Seven different T. gondii genotypes were identified, 6 of which were novel. Typing by 18S rRNA verified these 16 T. gondii-infected samples, and identified 1 Sarcocystis spp.-infected animal [Dasypus novemcinctus (nine-banded armadillo)]. The amplified T. gondii (GenBank accession No. L37415.1) and Sarcocystis spp. 18S rRNA products were confirmed by sequencing. Conclusions: Our results indicate that T. gondii is commonly present in wild mammals, which act as sources of infection for humans and animals, including other wild species. The approach employed herein proved useful for detecting T. gondii and Sarcocystis spp. in the environment and identifying their natural reservoirs, contributing to our understanding of host-parasite interactions. |
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Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, BrazilRoad-killed animalToxoplasma gondiiSarcocystis sppGenotypingMolecular techniquesIntroduction: Road-killed wild animals host zoonotic pathogens such as Toxoplasma gondii, offering a new opportunity for the epidemiological study of these infectious organisms. Methods: This investigation aimed to determine the presence of T. gondii and other apicomplexan parasites in tissue samples of 64 road-killed wild animals, using polymerase chain reaction (PCR). Positive samples were then typed by PCR-restriction fragment length polymorphism (RFLP) using 7 markers: SAG1, 5'-3' SAG2, SAG3, BTUB, c29-6, PK1, and Apico. PCR-RFLP targeting 18S ribosomal RNA (rRNA) genes was also performed on all samples to detect other apicomplexan parasites. Results: T. gondii DNA was detected in 16 tissue samples from 8 individual animals, as follows: 1 Cerdocyon thous (crab-eating fox), 1 Didelphis albiventris (white-eared opossum), 1 Lutreolina crassicaudata (lutrine opossum), 2 Myrmecophaga tridactyla (giant anteater), 1 Procyon cancrivorus (crab-eating raccoon), and 2 Sphiggurus spinosus (Paraguay hairy dwarf porcupine). Seven different T. gondii genotypes were identified, 6 of which were novel. Typing by 18S rRNA verified these 16 T. gondii-infected samples, and identified 1 Sarcocystis spp.-infected animal [Dasypus novemcinctus (nine-banded armadillo)]. The amplified T. gondii (GenBank accession No. L37415.1) and Sarcocystis spp. 18S rRNA products were confirmed by sequencing. Conclusions: Our results indicate that T. gondii is commonly present in wild mammals, which act as sources of infection for humans and animals, including other wild species. The approach employed herein proved useful for detecting T. gondii and Sarcocystis spp. in the environment and identifying their natural reservoirs, contributing to our understanding of host-parasite interactions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Adolfo Lutz Inst, Lab Reg Bauru, Sao Paulo, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP, BrazilMississippi State Univ, Dept Pathobiol & Populat Med, Coll Vet Med, Mississippi State, MS 39762 USAUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP, BrazilFAPESP: 08/09378-8FAPESP: 08/08291-6Soc Brasileira Medicina TropicalAdolfo Lutz InstUniversidade Estadual Paulista (Unesp)Mississippi State UnivRichini-Pereira, Virginia BodelaoMarson, Pamela Merlo [UNESP]Silva, Rodrigo Costa daLangoni, Helio [UNESP]2018-11-26T17:10:37Z2018-11-26T17:10:37Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article602-607application/pdfhttp://dx.doi.org/10.1590/0037-8682-0270-2016Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 49, n. 5, p. 602-607, 2016.0037-8682http://hdl.handle.net/11449/16215810.1590/0037-8682-0270-2016S0037-86822016000500602WOS:000387657500011S0037-86822016000500602.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Da Sociedade Brasileira De Medicina Tropical0,658info:eu-repo/semantics/openAccess2023-12-17T06:22:16Zoai:repositorio.unesp.br:11449/162158Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:35:21.993547Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
title |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
spellingShingle |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil Richini-Pereira, Virginia Bodelao Road-killed animal Toxoplasma gondii Sarcocystis spp Genotyping Molecular techniques |
title_short |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
title_full |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
title_fullStr |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
title_full_unstemmed |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
title_sort |
Genotyping of Toxoplasma gondii and Sarcocystis spp. in road-killed wild mammals from the Central Western Region of the State of Sao Paulo, Brazil |
author |
Richini-Pereira, Virginia Bodelao |
author_facet |
Richini-Pereira, Virginia Bodelao Marson, Pamela Merlo [UNESP] Silva, Rodrigo Costa da Langoni, Helio [UNESP] |
author_role |
author |
author2 |
Marson, Pamela Merlo [UNESP] Silva, Rodrigo Costa da Langoni, Helio [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Adolfo Lutz Inst Universidade Estadual Paulista (Unesp) Mississippi State Univ |
dc.contributor.author.fl_str_mv |
Richini-Pereira, Virginia Bodelao Marson, Pamela Merlo [UNESP] Silva, Rodrigo Costa da Langoni, Helio [UNESP] |
dc.subject.por.fl_str_mv |
Road-killed animal Toxoplasma gondii Sarcocystis spp Genotyping Molecular techniques |
topic |
Road-killed animal Toxoplasma gondii Sarcocystis spp Genotyping Molecular techniques |
description |
Introduction: Road-killed wild animals host zoonotic pathogens such as Toxoplasma gondii, offering a new opportunity for the epidemiological study of these infectious organisms. Methods: This investigation aimed to determine the presence of T. gondii and other apicomplexan parasites in tissue samples of 64 road-killed wild animals, using polymerase chain reaction (PCR). Positive samples were then typed by PCR-restriction fragment length polymorphism (RFLP) using 7 markers: SAG1, 5'-3' SAG2, SAG3, BTUB, c29-6, PK1, and Apico. PCR-RFLP targeting 18S ribosomal RNA (rRNA) genes was also performed on all samples to detect other apicomplexan parasites. Results: T. gondii DNA was detected in 16 tissue samples from 8 individual animals, as follows: 1 Cerdocyon thous (crab-eating fox), 1 Didelphis albiventris (white-eared opossum), 1 Lutreolina crassicaudata (lutrine opossum), 2 Myrmecophaga tridactyla (giant anteater), 1 Procyon cancrivorus (crab-eating raccoon), and 2 Sphiggurus spinosus (Paraguay hairy dwarf porcupine). Seven different T. gondii genotypes were identified, 6 of which were novel. Typing by 18S rRNA verified these 16 T. gondii-infected samples, and identified 1 Sarcocystis spp.-infected animal [Dasypus novemcinctus (nine-banded armadillo)]. The amplified T. gondii (GenBank accession No. L37415.1) and Sarcocystis spp. 18S rRNA products were confirmed by sequencing. Conclusions: Our results indicate that T. gondii is commonly present in wild mammals, which act as sources of infection for humans and animals, including other wild species. The approach employed herein proved useful for detecting T. gondii and Sarcocystis spp. in the environment and identifying their natural reservoirs, contributing to our understanding of host-parasite interactions. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-11-26T17:10:37Z 2018-11-26T17:10:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0270-2016 Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 49, n. 5, p. 602-607, 2016. 0037-8682 http://hdl.handle.net/11449/162158 10.1590/0037-8682-0270-2016 S0037-86822016000500602 WOS:000387657500011 S0037-86822016000500602.pdf |
url |
http://dx.doi.org/10.1590/0037-8682-0270-2016 http://hdl.handle.net/11449/162158 |
identifier_str_mv |
Revista Da Sociedade Brasileira De Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 49, n. 5, p. 602-607, 2016. 0037-8682 10.1590/0037-8682-0270-2016 S0037-86822016000500602 WOS:000387657500011 S0037-86822016000500602.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Da Sociedade Brasileira De Medicina Tropical 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
602-607 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129223244644352 |