Antibiótico antifungico produzido por um estreptomiceto da região de Araraquara

• Autor(a) principal: Bachiega, G. L. [UNESP]
• Data de Publicação: 2005
• Outros Autores: Vilegas, Wagner [UNESP], Ujikawa, Keidi [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/viewArticle/396; http://hdl.handle.net/11449/68636
• topic: Antibiotic Ara 4014-75; Antifungal; Pentaene; Screening; Streptomyces; antibiotic agent; antifungal agent; ara 4014 75; ara 404 75; unclassified drug; antimicrobial activity; Aspergillus oryzae; bacterial strain; Brazil; Candida albicans; chromatography; Escherichia coli; nonhuman; soil analysis; Staphylococcus aureus

With the significant increase in the incidence of invasive fungal infections during the last decade, mainly in patients with cancer, AIDS and other hospitalized patients who stay for long periods in intensive care units, there is an urgent need to screen for new antifungal agents possessing some advantages over known ones. This article reports a search in the field for a microorganism producing antibacterial and antifungal substances. Strains from soil samples collected in the region of Araraquara, Brazil, were isolated and analyzed for their antimicrobial potential against standard microorganisms (fungi Candida albicans and Aspergillus oryzae and bacteria Staphylococcus aureus and Escherichia coli). Out of the 64 strains isolated, 34 produced detectable antimicrobial activity. The streptomycete strain Ar4014 was chosen for further study, owing to its good antimicrobial activity against Candida albicans. Two of the fermentation media tested, 608-K and 602-B, were found to be best for the production and extraction of the antibiotic from Ar4014. After chromatographic separation of the crude extract on a silica column, the active fractions obtained showed UV-VIS absorption peaks characteristic of normal pentaenic antibiotics. The antibiotic was provisionally designated Ara 4014-75.