Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content

Detalhes bibliográficos
Autor(a) principal: Amaral, Vanessa Cristiane Santana [UNESP]
Data de Publicação: 2020
Outros Autores: Morais-Silva, Gessynger [UNESP], Laverde, Celina F. [UNESP], Marin, Marcelo T. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.neures.2020.07.002
http://hdl.handle.net/11449/199107
Resumo: Individual susceptibility to alcohol effects plays an important role in the development of alcohol addiction and studies have shown that glutamate release is altered after chronic ethanol consumption. The cystine-glutamate antiporter (xCT) is a protein that regulates glutamate release. However, little is known about the relationship between xCT levels and this individual susceptibility. Thus, this study aimed to evaluate the relationship between the extinction and stress-induced reinstatement of ethanol conditioned place preference (CPP) and xCT levels in the medial prefrontal cortex (mPFC), nucleus accumbens (NAcc) and amygdala (Amy). Male Swiss mice were submitted to a CPP procedure followed by an extinction protocol and then identified as those which extinguished the CPP and those that did not. In another cohort, mice that extinguished the CPP were submitted to a protocol of stress-induced reinstatement. Immediately after the tests, brains were removed for xCT quantification. The xCT levels were significantly lower in the mPFC and NAcc of mice that did not extinguish CPP. Moreover, mice that were susceptible to stress-induced reinstatement of CPP had lower levels of xCT in the NAcc. Our results suggest that individual susceptibility to the extinction and reinstatement of ethanol CPP is related to alterations in xCT levels.
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spelling Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter contentConditioned place preferenceCystine-glutamate antiporterEthanolMedial prefrontal cortexNucleus accumbensReinstatementIndividual susceptibility to alcohol effects plays an important role in the development of alcohol addiction and studies have shown that glutamate release is altered after chronic ethanol consumption. The cystine-glutamate antiporter (xCT) is a protein that regulates glutamate release. However, little is known about the relationship between xCT levels and this individual susceptibility. Thus, this study aimed to evaluate the relationship between the extinction and stress-induced reinstatement of ethanol conditioned place preference (CPP) and xCT levels in the medial prefrontal cortex (mPFC), nucleus accumbens (NAcc) and amygdala (Amy). Male Swiss mice were submitted to a CPP procedure followed by an extinction protocol and then identified as those which extinguished the CPP and those that did not. In another cohort, mice that extinguished the CPP were submitted to a protocol of stress-induced reinstatement. Immediately after the tests, brains were removed for xCT quantification. The xCT levels were significantly lower in the mPFC and NAcc of mice that did not extinguish CPP. Moreover, mice that were susceptible to stress-induced reinstatement of CPP had lower levels of xCT in the NAcc. Our results suggest that individual susceptibility to the extinction and reinstatement of ethanol CPP is related to alterations in xCT levels.Laboratory of Pharmacology and Toxicology of Natural and Synthetic Products State University of Goias Exact and Technological Sciences CampusSão Paulo State University (UNESP) School of Pharmaceutical Sciences Laboratory of PharmacologyJoint Graduate Program in Physiological Sciences (PIPGCF) UFSCar/UNESPSão Paulo State University (UNESP) School of Pharmaceutical Sciences Laboratory of PharmacologyJoint Graduate Program in Physiological Sciences (PIPGCF) UFSCar/UNESPExact and Technological Sciences CampusUniversidade Estadual Paulista (Unesp)Amaral, Vanessa Cristiane Santana [UNESP]Morais-Silva, Gessynger [UNESP]Laverde, Celina F. [UNESP]Marin, Marcelo T. [UNESP]2020-12-12T01:30:55Z2020-12-12T01:30:55Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.neures.2020.07.002Neuroscience Research.1872-81110168-0102http://hdl.handle.net/11449/19910710.1016/j.neures.2020.07.0022-s2.0-85087972598Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuroscience Researchinfo:eu-repo/semantics/openAccess2021-10-23T03:12:23Zoai:repositorio.unesp.br:11449/199107Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:31:17.644903Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
title Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
spellingShingle Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
Amaral, Vanessa Cristiane Santana [UNESP]
Conditioned place preference
Cystine-glutamate antiporter
Ethanol
Medial prefrontal cortex
Nucleus accumbens
Reinstatement
title_short Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
title_full Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
title_fullStr Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
title_full_unstemmed Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
title_sort Susceptibility to extinction and reinstatement of ethanol-induced conditioned place preference is related to differences in astrocyte cystine-glutamate antiporter content
author Amaral, Vanessa Cristiane Santana [UNESP]
author_facet Amaral, Vanessa Cristiane Santana [UNESP]
Morais-Silva, Gessynger [UNESP]
Laverde, Celina F. [UNESP]
Marin, Marcelo T. [UNESP]
author_role author
author2 Morais-Silva, Gessynger [UNESP]
Laverde, Celina F. [UNESP]
Marin, Marcelo T. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Exact and Technological Sciences Campus
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Amaral, Vanessa Cristiane Santana [UNESP]
Morais-Silva, Gessynger [UNESP]
Laverde, Celina F. [UNESP]
Marin, Marcelo T. [UNESP]
dc.subject.por.fl_str_mv Conditioned place preference
Cystine-glutamate antiporter
Ethanol
Medial prefrontal cortex
Nucleus accumbens
Reinstatement
topic Conditioned place preference
Cystine-glutamate antiporter
Ethanol
Medial prefrontal cortex
Nucleus accumbens
Reinstatement
description Individual susceptibility to alcohol effects plays an important role in the development of alcohol addiction and studies have shown that glutamate release is altered after chronic ethanol consumption. The cystine-glutamate antiporter (xCT) is a protein that regulates glutamate release. However, little is known about the relationship between xCT levels and this individual susceptibility. Thus, this study aimed to evaluate the relationship between the extinction and stress-induced reinstatement of ethanol conditioned place preference (CPP) and xCT levels in the medial prefrontal cortex (mPFC), nucleus accumbens (NAcc) and amygdala (Amy). Male Swiss mice were submitted to a CPP procedure followed by an extinction protocol and then identified as those which extinguished the CPP and those that did not. In another cohort, mice that extinguished the CPP were submitted to a protocol of stress-induced reinstatement. Immediately after the tests, brains were removed for xCT quantification. The xCT levels were significantly lower in the mPFC and NAcc of mice that did not extinguish CPP. Moreover, mice that were susceptible to stress-induced reinstatement of CPP had lower levels of xCT in the NAcc. Our results suggest that individual susceptibility to the extinction and reinstatement of ethanol CPP is related to alterations in xCT levels.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:30:55Z
2020-12-12T01:30:55Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.neures.2020.07.002
Neuroscience Research.
1872-8111
0168-0102
http://hdl.handle.net/11449/199107
10.1016/j.neures.2020.07.002
2-s2.0-85087972598
url http://dx.doi.org/10.1016/j.neures.2020.07.002
http://hdl.handle.net/11449/199107
identifier_str_mv Neuroscience Research.
1872-8111
0168-0102
10.1016/j.neures.2020.07.002
2-s2.0-85087972598
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neuroscience Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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