Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage

Detalhes bibliográficos
Autor(a) principal: Pinto, Maria Etelvina
Data de Publicação: 2010
Outros Autores: Botta, Livia Silva [UNESP], Taboga, Sebastiao Roberto [UNESP], Goes, Rejane Maira [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/ar.21050
http://hdl.handle.net/11449/21559
Resumo: This study describes the neonatal differentiation of the Mongolian gerbil gonocytes, focusing on the relationship between its relocation to the basement membrane, apoptosis and postrelocation changes and also the distribution of androgen receptors (AR). Testes of gerbils from I to 35 days of age (d) were examined by high resolution light microscopy and immunocytochemistry for proteins PCNA, VASA, and AR as well as by the TUNEL method. Gonocytes were quantified according to degree of relocation into nonrelocated, relocating and relocated. Most of them were found in the center of seminiferous cords at 1 d but a small number of relocating and relocated gonocytes were already visible in the first postnatal day. After relocation, gonocytes change phenotypically to a transitional stage designated herein prospermatogonia. Both gonocyte relocation and transformation into spermatogonial lineage occur asynchronously in the seminiferous cords, mainly after 7 d. Gonocyte proliferation began before but peak after their relocation to basement membrane at the prospermatogonia stage. Higher levels of gonocyte apoptosis were found at 7 d and 21 d. From this time onward gonocytes were not found. Gonocytes and prospermatogonia showed high amounts of AR in their cytoplasm contrary to spermatogonial subtypes, indicating a possible AR inactivation in these cells. In conclusion, the process of gonocyte relocation in the gerbil extends until the second postnatal week, leads to their rapid differentiation into prospermatogonia and occurs simultaneously with the loss of androgen sensitivity. Differently from other laboratory rodents, the events regarding gonocyte maturation in the gerbil last longer and occur asynchronously in seminiferous cords. Anat Rec, 293:310319, 2010. (C) 2009 Wiley-Liss, Inc.
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spelling Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stagegonocytestestisproliferationandrogen receptorMongolian gerbilThis study describes the neonatal differentiation of the Mongolian gerbil gonocytes, focusing on the relationship between its relocation to the basement membrane, apoptosis and postrelocation changes and also the distribution of androgen receptors (AR). Testes of gerbils from I to 35 days of age (d) were examined by high resolution light microscopy and immunocytochemistry for proteins PCNA, VASA, and AR as well as by the TUNEL method. Gonocytes were quantified according to degree of relocation into nonrelocated, relocating and relocated. Most of them were found in the center of seminiferous cords at 1 d but a small number of relocating and relocated gonocytes were already visible in the first postnatal day. After relocation, gonocytes change phenotypically to a transitional stage designated herein prospermatogonia. Both gonocyte relocation and transformation into spermatogonial lineage occur asynchronously in the seminiferous cords, mainly after 7 d. Gonocyte proliferation began before but peak after their relocation to basement membrane at the prospermatogonia stage. Higher levels of gonocyte apoptosis were found at 7 d and 21 d. From this time onward gonocytes were not found. Gonocytes and prospermatogonia showed high amounts of AR in their cytoplasm contrary to spermatogonial subtypes, indicating a possible AR inactivation in these cells. In conclusion, the process of gonocyte relocation in the gerbil extends until the second postnatal week, leads to their rapid differentiation into prospermatogonia and occurs simultaneously with the loss of androgen sensitivity. Differently from other laboratory rodents, the events regarding gonocyte maturation in the gerbil last longer and occur asynchronously in seminiferous cords. Anat Rec, 293:310319, 2010. (C) 2009 Wiley-Liss, Inc.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)State Univ Campinas UNICAMP, Inst Biol, Dept Cell Biol, Campinas, SP, BrazilSão Paulo State Univ IBILCE UNESP, Dept Biol, Sao Jose do Rio Preto, SP, BrazilSão Paulo State Univ IBILCE UNESP, Dept Biol, Sao Jose do Rio Preto, SP, BrazilCNPq: 12/069/2008-7FAPESP: 06-07008Wiley-lissUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Pinto, Maria EtelvinaBotta, Livia Silva [UNESP]Taboga, Sebastiao Roberto [UNESP]Goes, Rejane Maira [UNESP]2014-05-20T14:01:01Z2014-05-20T14:01:01Z2010-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article310-319http://dx.doi.org/10.1002/ar.21050Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 293, n. 2, p. 310-319, 2010.1932-8486http://hdl.handle.net/11449/2155910.1002/ar.21050WOS:000274702300014144525946852618809471933473121570000-0002-0970-42880000-0002-3622-460XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnatomical Record-advances In Integrative Anatomy and Evolutionary Biology1.3730,766info:eu-repo/semantics/openAccess2021-10-22T16:54:12Zoai:repositorio.unesp.br:11449/21559Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:16:24.994754Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
title Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
spellingShingle Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
Pinto, Maria Etelvina
gonocytes
testis
proliferation
androgen receptor
Mongolian gerbil
title_short Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
title_full Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
title_fullStr Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
title_full_unstemmed Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
title_sort Neonatal Gonocyte Differentiation in Mongolian Gerbil Meriones unguiculatus Involves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage
author Pinto, Maria Etelvina
author_facet Pinto, Maria Etelvina
Botta, Livia Silva [UNESP]
Taboga, Sebastiao Roberto [UNESP]
Goes, Rejane Maira [UNESP]
author_role author
author2 Botta, Livia Silva [UNESP]
Taboga, Sebastiao Roberto [UNESP]
Goes, Rejane Maira [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pinto, Maria Etelvina
Botta, Livia Silva [UNESP]
Taboga, Sebastiao Roberto [UNESP]
Goes, Rejane Maira [UNESP]
dc.subject.por.fl_str_mv gonocytes
testis
proliferation
androgen receptor
Mongolian gerbil
topic gonocytes
testis
proliferation
androgen receptor
Mongolian gerbil
description This study describes the neonatal differentiation of the Mongolian gerbil gonocytes, focusing on the relationship between its relocation to the basement membrane, apoptosis and postrelocation changes and also the distribution of androgen receptors (AR). Testes of gerbils from I to 35 days of age (d) were examined by high resolution light microscopy and immunocytochemistry for proteins PCNA, VASA, and AR as well as by the TUNEL method. Gonocytes were quantified according to degree of relocation into nonrelocated, relocating and relocated. Most of them were found in the center of seminiferous cords at 1 d but a small number of relocating and relocated gonocytes were already visible in the first postnatal day. After relocation, gonocytes change phenotypically to a transitional stage designated herein prospermatogonia. Both gonocyte relocation and transformation into spermatogonial lineage occur asynchronously in the seminiferous cords, mainly after 7 d. Gonocyte proliferation began before but peak after their relocation to basement membrane at the prospermatogonia stage. Higher levels of gonocyte apoptosis were found at 7 d and 21 d. From this time onward gonocytes were not found. Gonocytes and prospermatogonia showed high amounts of AR in their cytoplasm contrary to spermatogonial subtypes, indicating a possible AR inactivation in these cells. In conclusion, the process of gonocyte relocation in the gerbil extends until the second postnatal week, leads to their rapid differentiation into prospermatogonia and occurs simultaneously with the loss of androgen sensitivity. Differently from other laboratory rodents, the events regarding gonocyte maturation in the gerbil last longer and occur asynchronously in seminiferous cords. Anat Rec, 293:310319, 2010. (C) 2009 Wiley-Liss, Inc.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
2014-05-20T14:01:01Z
2014-05-20T14:01:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/ar.21050
Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 293, n. 2, p. 310-319, 2010.
1932-8486
http://hdl.handle.net/11449/21559
10.1002/ar.21050
WOS:000274702300014
1445259468526188
0947193347312157
0000-0002-0970-4288
0000-0002-3622-460X
url http://dx.doi.org/10.1002/ar.21050
http://hdl.handle.net/11449/21559
identifier_str_mv Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 293, n. 2, p. 310-319, 2010.
1932-8486
10.1002/ar.21050
WOS:000274702300014
1445259468526188
0947193347312157
0000-0002-0970-4288
0000-0002-3622-460X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 310-319
dc.publisher.none.fl_str_mv Wiley-liss
publisher.none.fl_str_mv Wiley-liss
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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