Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.domaniend.2022.106751 http://hdl.handle.net/11449/245886 |
Resumo: | This study investigated the effectiveness of different doses of estradiol benzoate (EB) to promote cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. Multiparous (MULT) and nulliparous (NULL) crossbred Lacaune X Santa Inês ewes were superovulated and naturally bred. Seven days after progesterone withdrawal, females were randomly assigned to one of three distinct cervical relaxation protocols, consisting of i.m. treatment with 37.5 µg d-cloprostenol and different doses of EB: 0.0 mg (0.0EB group; n = 3 NULL and 14 MULT); 0.5 mg (0.5EB group; n = 4 NULL and 12 MULT) or 1.0 mg (1.0EB group, n = 6 NULL and 11 MULT) 16 h before NSER. All ewes received 50 IU of oxytocin 20 min before NSER (D17). Blood samples were collected and ultrasound exams (B-mode and color Doppler) were performed at two timepoints: immediately before d-cloprostenol and EB treatments and prior to NSER. Estrous behavior, corpora lutea count and NSER success outcomes were not affected by EB treatments nor parity (P > 0.05). Embryo recovery rate was greater for ewes in the 0.5EB group and in the NULL ewes (P < 0.05). Ovarian biometrics differed between the two evaluation timepoints in all groups (P < 0.05). Plasma estradiol increased over time, reaching a significant greater level in 1.0EB ewes compared to controls on D17 (P < 0.05), whereas progesterone concentrations decreased over time in all groups (P > 0.05). In conclusion, treatments did not affect NSER success but they did affect luteal function by altering P4 and E2 concentrations. Therefore, the NSER technique can be successfully performed in ewes with or without prior treatment with EB. |
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Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewesCervical dilationNSERProgesteroneSheepThis study investigated the effectiveness of different doses of estradiol benzoate (EB) to promote cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. Multiparous (MULT) and nulliparous (NULL) crossbred Lacaune X Santa Inês ewes were superovulated and naturally bred. Seven days after progesterone withdrawal, females were randomly assigned to one of three distinct cervical relaxation protocols, consisting of i.m. treatment with 37.5 µg d-cloprostenol and different doses of EB: 0.0 mg (0.0EB group; n = 3 NULL and 14 MULT); 0.5 mg (0.5EB group; n = 4 NULL and 12 MULT) or 1.0 mg (1.0EB group, n = 6 NULL and 11 MULT) 16 h before NSER. All ewes received 50 IU of oxytocin 20 min before NSER (D17). Blood samples were collected and ultrasound exams (B-mode and color Doppler) were performed at two timepoints: immediately before d-cloprostenol and EB treatments and prior to NSER. Estrous behavior, corpora lutea count and NSER success outcomes were not affected by EB treatments nor parity (P > 0.05). Embryo recovery rate was greater for ewes in the 0.5EB group and in the NULL ewes (P < 0.05). Ovarian biometrics differed between the two evaluation timepoints in all groups (P < 0.05). Plasma estradiol increased over time, reaching a significant greater level in 1.0EB ewes compared to controls on D17 (P < 0.05), whereas progesterone concentrations decreased over time in all groups (P > 0.05). In conclusion, treatments did not affect NSER success but they did affect luteal function by altering P4 and E2 concentrations. Therefore, the NSER technique can be successfully performed in ewes with or without prior treatment with EB.Universidade Federal de Viçosa Av. Peter Henry Rolfs, s/n, 36570-000Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Via de acesso Prof. Paulo Donato Castellane, s/n, 14884-900Universidade Federal Rural do Rio de Janeiro Rodovia BR 465, Km7, s/n, 23890-000Universidade Federal Fluminense Rua Vital Brazil Filho, 64, 24230-340Universidade Estadual do Norte Fluminense Darcy Ribeiro Av. Alberto Lamego, n° 2000, 28013-642Embrapa Gado de Leite Av. Eugênio do Nascimento, 610, 36038-330Embrapa Caprinos e Ovinos Três Lagoas Farm, Road Sobral –Groaíras, km 04, CP 145, 62010-970Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Via de acesso Prof. Paulo Donato Castellane, s/n, 14884-900Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (UNESP)Rodovia BR 465Universidade Federal Fluminense (UFF)Av. Alberto LamegoEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Dias, J. H.Gonçalves, J. D. [UNESP]Arrais, A. M.Souza-Fabjan, J. M.G.Bastos, R.Batista, R. I.T.P.Siqueira, L. G.B.Oliveira, M. E.F. [UNESP]Fonseca, J. F.2023-07-29T12:25:54Z2023-07-29T12:25:54Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.domaniend.2022.106751Domestic Animal Endocrinology, v. 82.0739-7240http://hdl.handle.net/11449/24588610.1016/j.domaniend.2022.1067512-s2.0-85135693214Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDomestic Animal Endocrinologyinfo:eu-repo/semantics/openAccess2023-07-29T12:25:54Zoai:repositorio.unesp.br:11449/245886Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:25:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
title |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
spellingShingle |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes Dias, J. H. Cervical dilation NSER Progesterone Sheep |
title_short |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
title_full |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
title_fullStr |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
title_full_unstemmed |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
title_sort |
Effects of different doses of estradiol benzoate used in a cervical relaxation protocol on the success of non-surgical embryo recovery and luteal function in superovulated ewes |
author |
Dias, J. H. |
author_facet |
Dias, J. H. Gonçalves, J. D. [UNESP] Arrais, A. M. Souza-Fabjan, J. M.G. Bastos, R. Batista, R. I.T.P. Siqueira, L. G.B. Oliveira, M. E.F. [UNESP] Fonseca, J. F. |
author_role |
author |
author2 |
Gonçalves, J. D. [UNESP] Arrais, A. M. Souza-Fabjan, J. M.G. Bastos, R. Batista, R. I.T.P. Siqueira, L. G.B. Oliveira, M. E.F. [UNESP] Fonseca, J. F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (UNESP) Rodovia BR 465 Universidade Federal Fluminense (UFF) Av. Alberto Lamego Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Dias, J. H. Gonçalves, J. D. [UNESP] Arrais, A. M. Souza-Fabjan, J. M.G. Bastos, R. Batista, R. I.T.P. Siqueira, L. G.B. Oliveira, M. E.F. [UNESP] Fonseca, J. F. |
dc.subject.por.fl_str_mv |
Cervical dilation NSER Progesterone Sheep |
topic |
Cervical dilation NSER Progesterone Sheep |
description |
This study investigated the effectiveness of different doses of estradiol benzoate (EB) to promote cervical relaxation and their effects on luteal function and outcomes of non-surgical embryo recovery (NSER) in sheep. Multiparous (MULT) and nulliparous (NULL) crossbred Lacaune X Santa Inês ewes were superovulated and naturally bred. Seven days after progesterone withdrawal, females were randomly assigned to one of three distinct cervical relaxation protocols, consisting of i.m. treatment with 37.5 µg d-cloprostenol and different doses of EB: 0.0 mg (0.0EB group; n = 3 NULL and 14 MULT); 0.5 mg (0.5EB group; n = 4 NULL and 12 MULT) or 1.0 mg (1.0EB group, n = 6 NULL and 11 MULT) 16 h before NSER. All ewes received 50 IU of oxytocin 20 min before NSER (D17). Blood samples were collected and ultrasound exams (B-mode and color Doppler) were performed at two timepoints: immediately before d-cloprostenol and EB treatments and prior to NSER. Estrous behavior, corpora lutea count and NSER success outcomes were not affected by EB treatments nor parity (P > 0.05). Embryo recovery rate was greater for ewes in the 0.5EB group and in the NULL ewes (P < 0.05). Ovarian biometrics differed between the two evaluation timepoints in all groups (P < 0.05). Plasma estradiol increased over time, reaching a significant greater level in 1.0EB ewes compared to controls on D17 (P < 0.05), whereas progesterone concentrations decreased over time in all groups (P > 0.05). In conclusion, treatments did not affect NSER success but they did affect luteal function by altering P4 and E2 concentrations. Therefore, the NSER technique can be successfully performed in ewes with or without prior treatment with EB. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:25:54Z 2023-07-29T12:25:54Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.domaniend.2022.106751 Domestic Animal Endocrinology, v. 82. 0739-7240 http://hdl.handle.net/11449/245886 10.1016/j.domaniend.2022.106751 2-s2.0-85135693214 |
url |
http://dx.doi.org/10.1016/j.domaniend.2022.106751 http://hdl.handle.net/11449/245886 |
identifier_str_mv |
Domestic Animal Endocrinology, v. 82. 0739-7240 10.1016/j.domaniend.2022.106751 2-s2.0-85135693214 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Domestic Animal Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965459669319680 |