Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet

Detalhes bibliográficos
Autor(a) principal: Santos e Cunha, Michel
Data de Publicação: 2019
Outros Autores: Bonato, Denis Vinicius, Vrisman, Dayane Priscila [UNESP], Taira, Augusto Ryonosuke [UNESP], Bueno Borges, Luisa Pucci, Albuquerque, Rodrigo dos Santos, Coutinho, Leandro Nassar, Hilario da Silva, Marlon Richard, Neumann, Mikael, Maia Teixeira, Pedro Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.livsci.2019.04.006
http://hdl.handle.net/11449/184542
Resumo: The objective of the present study was to carry out the andrological evaluation of young bulls fed a hyper-energetic diet by correlating traditional andrological data with the results of ultrasonographic and histological tests. The animals were kept in confinement for 94 days with an adaptation period of ten days. Three evaluations were performed with 28 day intervals between each. The animals were weighed and testicular morphometry, semen analysis, ultrasonographic testicular evaluation with a visual scale using the histogram method and histopathological evaluation were performed. Ponderal data, testicular biometry, sperm parameters and ultrasound data were not closely correlated after 94 days (r(2) < 0.3). Body weight (BW), scrotal circumference (SC) and testicular volume (TV) varied significantly across all the time periods (D28, D56 and D84). Visual ultrasound analysis revealed that the testicular stroma were homogenous until the 28th day, with minimal changes in echogenicity. By day 56, although the images remained homogeneous, hypoechoic areas had appeared. From the 84th day heterogenicity of the testicular stroma was observed in some animals. These included hypoechoic and hyperechoic areas, sometimes with both patterns in the same testis. Significant differences were observed between the visual and histological ultrasonographic analysis at the beginning of the experiment and on the 56th and 84th days (P < 0.05). The histological evaluation revealed several alterations which indicated inflammatory processes, such as the presence of fibrous connective tissue, as well as the atrophy and vacuolization of the cytoplasm, resulting in cellular degeneration. While nutritional management employing a hyperenergetic diet encouraged testicular and ponderal growth, it also resulted in the appearance of pathologies and visible changes to the echogenicity of the testicular stroma, confirmed by histological evaluation, which led to spermatogenesis disorders.
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spelling Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic dietUltrasoundTesticular stromaHistologyDegenerationCattleThe objective of the present study was to carry out the andrological evaluation of young bulls fed a hyper-energetic diet by correlating traditional andrological data with the results of ultrasonographic and histological tests. The animals were kept in confinement for 94 days with an adaptation period of ten days. Three evaluations were performed with 28 day intervals between each. The animals were weighed and testicular morphometry, semen analysis, ultrasonographic testicular evaluation with a visual scale using the histogram method and histopathological evaluation were performed. Ponderal data, testicular biometry, sperm parameters and ultrasound data were not closely correlated after 94 days (r(2) < 0.3). Body weight (BW), scrotal circumference (SC) and testicular volume (TV) varied significantly across all the time periods (D28, D56 and D84). Visual ultrasound analysis revealed that the testicular stroma were homogenous until the 28th day, with minimal changes in echogenicity. By day 56, although the images remained homogeneous, hypoechoic areas had appeared. From the 84th day heterogenicity of the testicular stroma was observed in some animals. These included hypoechoic and hyperechoic areas, sometimes with both patterns in the same testis. Significant differences were observed between the visual and histological ultrasonographic analysis at the beginning of the experiment and on the 56th and 84th days (P < 0.05). The histological evaluation revealed several alterations which indicated inflammatory processes, such as the presence of fibrous connective tissue, as well as the atrophy and vacuolization of the cytoplasm, resulting in cellular degeneration. While nutritional management employing a hyperenergetic diet encouraged testicular and ponderal growth, it also resulted in the appearance of pathologies and visible changes to the echogenicity of the testicular stroma, confirmed by histological evaluation, which led to spermatogenesis disorders.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Franca, BR-14404600 Franca, SP, BrazilSao Paulo State Univ, Coll Agr Sci & Vet Med, BR-14884900 Jaboticabal, SP, BrazilAmazonia Rural Fed Univ, Anim Hlth & Prod Inst, Belem, Para, BrazilMidwest State Univ, Dept Vet, BR-85040080 Guarapuava, Parana, BrazilPara Fed Univ, Vet Med Inst, BR-68740970 Castanhal, Para, BrazilSao Paulo State Univ, Coll Agr Sci & Vet Med, BR-14884900 Jaboticabal, SP, BrazilElsevier B.V.Univ FrancaUniversidade Estadual Paulista (Unesp)Amazonia Rural Fed UnivMidwest State UnivPara Fed UnivSantos e Cunha, MichelBonato, Denis ViniciusVrisman, Dayane Priscila [UNESP]Taira, Augusto Ryonosuke [UNESP]Bueno Borges, Luisa PucciAlbuquerque, Rodrigo dos SantosCoutinho, Leandro NassarHilario da Silva, Marlon RichardNeumann, MikaelMaia Teixeira, Pedro Paulo2019-10-04T12:14:32Z2019-10-04T12:14:32Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article85-90http://dx.doi.org/10.1016/j.livsci.2019.04.006Livestock Science. Amsterdam: Elsevier Science Bv, v. 225, p. 85-90, 2019.1871-1413http://hdl.handle.net/11449/18454210.1016/j.livsci.2019.04.006WOS:000472698000012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2021-10-22T23:59:54Zoai:repositorio.unesp.br:11449/184542Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:56:09.545256Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
title Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
spellingShingle Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
Santos e Cunha, Michel
Ultrasound
Testicular stroma
Histology
Degeneration
Cattle
title_short Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
title_full Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
title_fullStr Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
title_full_unstemmed Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
title_sort Histopathological and ultrasonographic testicular evaluation of young bulls fed a hyperenergetic diet
author Santos e Cunha, Michel
author_facet Santos e Cunha, Michel
Bonato, Denis Vinicius
Vrisman, Dayane Priscila [UNESP]
Taira, Augusto Ryonosuke [UNESP]
Bueno Borges, Luisa Pucci
Albuquerque, Rodrigo dos Santos
Coutinho, Leandro Nassar
Hilario da Silva, Marlon Richard
Neumann, Mikael
Maia Teixeira, Pedro Paulo
author_role author
author2 Bonato, Denis Vinicius
Vrisman, Dayane Priscila [UNESP]
Taira, Augusto Ryonosuke [UNESP]
Bueno Borges, Luisa Pucci
Albuquerque, Rodrigo dos Santos
Coutinho, Leandro Nassar
Hilario da Silva, Marlon Richard
Neumann, Mikael
Maia Teixeira, Pedro Paulo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Franca
Universidade Estadual Paulista (Unesp)
Amazonia Rural Fed Univ
Midwest State Univ
Para Fed Univ
dc.contributor.author.fl_str_mv Santos e Cunha, Michel
Bonato, Denis Vinicius
Vrisman, Dayane Priscila [UNESP]
Taira, Augusto Ryonosuke [UNESP]
Bueno Borges, Luisa Pucci
Albuquerque, Rodrigo dos Santos
Coutinho, Leandro Nassar
Hilario da Silva, Marlon Richard
Neumann, Mikael
Maia Teixeira, Pedro Paulo
dc.subject.por.fl_str_mv Ultrasound
Testicular stroma
Histology
Degeneration
Cattle
topic Ultrasound
Testicular stroma
Histology
Degeneration
Cattle
description The objective of the present study was to carry out the andrological evaluation of young bulls fed a hyper-energetic diet by correlating traditional andrological data with the results of ultrasonographic and histological tests. The animals were kept in confinement for 94 days with an adaptation period of ten days. Three evaluations were performed with 28 day intervals between each. The animals were weighed and testicular morphometry, semen analysis, ultrasonographic testicular evaluation with a visual scale using the histogram method and histopathological evaluation were performed. Ponderal data, testicular biometry, sperm parameters and ultrasound data were not closely correlated after 94 days (r(2) < 0.3). Body weight (BW), scrotal circumference (SC) and testicular volume (TV) varied significantly across all the time periods (D28, D56 and D84). Visual ultrasound analysis revealed that the testicular stroma were homogenous until the 28th day, with minimal changes in echogenicity. By day 56, although the images remained homogeneous, hypoechoic areas had appeared. From the 84th day heterogenicity of the testicular stroma was observed in some animals. These included hypoechoic and hyperechoic areas, sometimes with both patterns in the same testis. Significant differences were observed between the visual and histological ultrasonographic analysis at the beginning of the experiment and on the 56th and 84th days (P < 0.05). The histological evaluation revealed several alterations which indicated inflammatory processes, such as the presence of fibrous connective tissue, as well as the atrophy and vacuolization of the cytoplasm, resulting in cellular degeneration. While nutritional management employing a hyperenergetic diet encouraged testicular and ponderal growth, it also resulted in the appearance of pathologies and visible changes to the echogenicity of the testicular stroma, confirmed by histological evaluation, which led to spermatogenesis disorders.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:14:32Z
2019-10-04T12:14:32Z
2019-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.livsci.2019.04.006
Livestock Science. Amsterdam: Elsevier Science Bv, v. 225, p. 85-90, 2019.
1871-1413
http://hdl.handle.net/11449/184542
10.1016/j.livsci.2019.04.006
WOS:000472698000012
url http://dx.doi.org/10.1016/j.livsci.2019.04.006
http://hdl.handle.net/11449/184542
identifier_str_mv Livestock Science. Amsterdam: Elsevier Science Bv, v. 225, p. 85-90, 2019.
1871-1413
10.1016/j.livsci.2019.04.006
WOS:000472698000012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Livestock Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 85-90
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129475356917760