Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00299-018-2310-9 http://hdl.handle.net/11449/179952 |
Resumo: | Key message: A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. Abstract: In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5′-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool. |
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Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomatoCoffeePromoterSeed maturation proteinSeed-specific expressionTomatoKey message: A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. Abstract: In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5′-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Genetics Institute of Biosciences Sao Paulo State University (UNESP)Department of Biological Sciences (LCB) Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ) University of Sao Paulo (USP)Embrapa Coffee and Coffee Center Alcides Carvalho Agronomic Institute of CampinasDepartment of Genetics Institute of Biosciences Sao Paulo State University (UNESP)FAPESP: 2009/54361-9Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Quintero, Fabíola OCampo [UNESP]Pinto, Layra G. [UNESP]Barsalobres-Cavallari, Carla F. [UNESP]Arcuri, Mariana de Lara Campos [UNESP]Pino, Lilian EllenPeres, Lázaro Eustáquio PereiraMaluf, Mirian P.Maia, Ivan G. [UNESP]2018-12-11T17:37:26Z2018-12-11T17:37:26Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1257-1268application/pdfhttp://dx.doi.org/10.1007/s00299-018-2310-9Plant Cell Reports, v. 37, n. 9, p. 1257-1268, 2018.0721-7714http://hdl.handle.net/11449/17995210.1007/s00299-018-2310-92-s2.0-850485027372-s2.0-85048502737.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Cell Reports1,291info:eu-repo/semantics/openAccess2023-12-21T06:23:02Zoai:repositorio.unesp.br:11449/179952Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-21T06:23:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
title |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
spellingShingle |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato Quintero, Fabíola OCampo [UNESP] Coffee Promoter Seed maturation protein Seed-specific expression Tomato |
title_short |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
title_full |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
title_fullStr |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
title_full_unstemmed |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
title_sort |
Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato |
author |
Quintero, Fabíola OCampo [UNESP] |
author_facet |
Quintero, Fabíola OCampo [UNESP] Pinto, Layra G. [UNESP] Barsalobres-Cavallari, Carla F. [UNESP] Arcuri, Mariana de Lara Campos [UNESP] Pino, Lilian Ellen Peres, Lázaro Eustáquio Pereira Maluf, Mirian P. Maia, Ivan G. [UNESP] |
author_role |
author |
author2 |
Pinto, Layra G. [UNESP] Barsalobres-Cavallari, Carla F. [UNESP] Arcuri, Mariana de Lara Campos [UNESP] Pino, Lilian Ellen Peres, Lázaro Eustáquio Pereira Maluf, Mirian P. Maia, Ivan G. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Quintero, Fabíola OCampo [UNESP] Pinto, Layra G. [UNESP] Barsalobres-Cavallari, Carla F. [UNESP] Arcuri, Mariana de Lara Campos [UNESP] Pino, Lilian Ellen Peres, Lázaro Eustáquio Pereira Maluf, Mirian P. Maia, Ivan G. [UNESP] |
dc.subject.por.fl_str_mv |
Coffee Promoter Seed maturation protein Seed-specific expression Tomato |
topic |
Coffee Promoter Seed maturation protein Seed-specific expression Tomato |
description |
Key message: A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. Abstract: In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5′-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:26Z 2018-12-11T17:37:26Z 2018-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00299-018-2310-9 Plant Cell Reports, v. 37, n. 9, p. 1257-1268, 2018. 0721-7714 http://hdl.handle.net/11449/179952 10.1007/s00299-018-2310-9 2-s2.0-85048502737 2-s2.0-85048502737.pdf |
url |
http://dx.doi.org/10.1007/s00299-018-2310-9 http://hdl.handle.net/11449/179952 |
identifier_str_mv |
Plant Cell Reports, v. 37, n. 9, p. 1257-1268, 2018. 0721-7714 10.1007/s00299-018-2310-9 2-s2.0-85048502737 2-s2.0-85048502737.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Cell Reports 1,291 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1257-1268 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965359398191104 |