Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2021v42n2p695 http://hdl.handle.net/11449/205979 |
Resumo: | Neonatal diarrhea is amongst the most frequent diseases affecting calves, leading to damages in milk production. Although Escherichia coli is a commensal microorganism in the gastrointestinal tract, some pathotypes are known to cause high prevalence of diarrhea and food poisoning. The rapidly increasing resistance of bacteria to antimicrobials leads to the research in new, alternative treatment options. The present study aimed at the detection of E. coli pathotypes in newborn diarrheic and non-diarrheic calves, as well as susceptibility tests for antimicrobials and vegetal extracts. Samples were collected from animals located in dairy farms in the state of Minas Gerais, Brazil. The samples were sent for microbiological isolation, genetic identification using PCR and antimicrobial tests. A total of 35 strains from diarrheic animals tested positive for at least one of the virulence genes analyzed: stx1, stx2, eae, bfp and sta. As for the non-diarrheic animals, 9 isolated strains possessed one of or both stx1 and stx2 genes, classifying these non-diarrheic cattle as reservoirs for the STEC pathotype and possible biological vectors of this pathogen. Regarding the susceptibility tests, most isolates displayed resistance to multiple antimicrobial classes. Among the vegetal extracts tested, all isolates tested sensitive to the active ingredient of Salvia officinalis L. (sage). Showcasing an alternative tool to aid in the treatment of pathogenic bacteria. |
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Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calvesPerfil de susceptibilidade e virulência de patótipos de Escherichia coli isolados de bezerros diarreicos e nãodiarreicosColibacillosisEPECETECSalvia officinalis LSTECNeonatal diarrhea is amongst the most frequent diseases affecting calves, leading to damages in milk production. Although Escherichia coli is a commensal microorganism in the gastrointestinal tract, some pathotypes are known to cause high prevalence of diarrhea and food poisoning. The rapidly increasing resistance of bacteria to antimicrobials leads to the research in new, alternative treatment options. The present study aimed at the detection of E. coli pathotypes in newborn diarrheic and non-diarrheic calves, as well as susceptibility tests for antimicrobials and vegetal extracts. Samples were collected from animals located in dairy farms in the state of Minas Gerais, Brazil. The samples were sent for microbiological isolation, genetic identification using PCR and antimicrobial tests. A total of 35 strains from diarrheic animals tested positive for at least one of the virulence genes analyzed: stx1, stx2, eae, bfp and sta. As for the non-diarrheic animals, 9 isolated strains possessed one of or both stx1 and stx2 genes, classifying these non-diarrheic cattle as reservoirs for the STEC pathotype and possible biological vectors of this pathogen. Regarding the susceptibility tests, most isolates displayed resistance to multiple antimicrobial classes. Among the vegetal extracts tested, all isolates tested sensitive to the active ingredient of Salvia officinalis L. (sage). Showcasing an alternative tool to aid in the treatment of pathogenic bacteria.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The Agropecuary Microbiology Postgraduate Program São Paulo State University School of Agricultural and Veterinary Studies UNESP/FCAVAgropecuary Microbiology Postgraduate Program UNESP/FCAVThe Animal Science Postgraduate Program José do Rosário Vellano University UNIFENASUniversity of Campinas School of Dentistry Unicamp/FOPThe Agropecuary Microbiology Postgraduate Program São Paulo State University School of Agricultural and Veterinary Studies UNESP/FCAVAgropecuary Microbiology Postgraduate Program UNESP/FCAVUniversidade Estadual Paulista (Unesp)UNIFENASUniversidade Estadual de Campinas (UNICAMP)da Silva Menezes Azola, Juliana [UNESP]Cardozo, Marita Vedovelli [UNESP]de Souza Moreira, Biancado Nascimento, Gabriel Michelutti [UNESP]Boriollo, Marcelo Fabiano Gomesde Ávila, Fernando Antônio [UNESP]2021-06-25T10:24:36Z2021-06-25T10:24:36Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article695-706http://dx.doi.org/10.5433/1679-0359.2021v42n2p695Semina:Ciencias Agrarias, v. 42, n. 2, p. 695-706, 2021.1679-03591676-546Xhttp://hdl.handle.net/11449/20597910.5433/1679-0359.2021v42n2p6952-s2.0-85101828030Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina:Ciencias Agrariasinfo:eu-repo/semantics/openAccess2022-02-10T01:13:51Zoai:repositorio.unesp.br:11449/205979Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:21:32.738920Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves Perfil de susceptibilidade e virulência de patótipos de Escherichia coli isolados de bezerros diarreicos e nãodiarreicos |
title |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
spellingShingle |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves da Silva Menezes Azola, Juliana [UNESP] Colibacillosis EPEC ETEC Salvia officinalis L STEC |
title_short |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
title_full |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
title_fullStr |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
title_full_unstemmed |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
title_sort |
Susceptibility and virulence profile of Escherichia coli pathotypes isolated from diarrheic and non-diarrheic calves |
author |
da Silva Menezes Azola, Juliana [UNESP] |
author_facet |
da Silva Menezes Azola, Juliana [UNESP] Cardozo, Marita Vedovelli [UNESP] de Souza Moreira, Bianca do Nascimento, Gabriel Michelutti [UNESP] Boriollo, Marcelo Fabiano Gomes de Ávila, Fernando Antônio [UNESP] |
author_role |
author |
author2 |
Cardozo, Marita Vedovelli [UNESP] de Souza Moreira, Bianca do Nascimento, Gabriel Michelutti [UNESP] Boriollo, Marcelo Fabiano Gomes de Ávila, Fernando Antônio [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIFENAS Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
da Silva Menezes Azola, Juliana [UNESP] Cardozo, Marita Vedovelli [UNESP] de Souza Moreira, Bianca do Nascimento, Gabriel Michelutti [UNESP] Boriollo, Marcelo Fabiano Gomes de Ávila, Fernando Antônio [UNESP] |
dc.subject.por.fl_str_mv |
Colibacillosis EPEC ETEC Salvia officinalis L STEC |
topic |
Colibacillosis EPEC ETEC Salvia officinalis L STEC |
description |
Neonatal diarrhea is amongst the most frequent diseases affecting calves, leading to damages in milk production. Although Escherichia coli is a commensal microorganism in the gastrointestinal tract, some pathotypes are known to cause high prevalence of diarrhea and food poisoning. The rapidly increasing resistance of bacteria to antimicrobials leads to the research in new, alternative treatment options. The present study aimed at the detection of E. coli pathotypes in newborn diarrheic and non-diarrheic calves, as well as susceptibility tests for antimicrobials and vegetal extracts. Samples were collected from animals located in dairy farms in the state of Minas Gerais, Brazil. The samples were sent for microbiological isolation, genetic identification using PCR and antimicrobial tests. A total of 35 strains from diarrheic animals tested positive for at least one of the virulence genes analyzed: stx1, stx2, eae, bfp and sta. As for the non-diarrheic animals, 9 isolated strains possessed one of or both stx1 and stx2 genes, classifying these non-diarrheic cattle as reservoirs for the STEC pathotype and possible biological vectors of this pathogen. Regarding the susceptibility tests, most isolates displayed resistance to multiple antimicrobial classes. Among the vegetal extracts tested, all isolates tested sensitive to the active ingredient of Salvia officinalis L. (sage). Showcasing an alternative tool to aid in the treatment of pathogenic bacteria. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:24:36Z 2021-06-25T10:24:36Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2021v42n2p695 Semina:Ciencias Agrarias, v. 42, n. 2, p. 695-706, 2021. 1679-0359 1676-546X http://hdl.handle.net/11449/205979 10.5433/1679-0359.2021v42n2p695 2-s2.0-85101828030 |
url |
http://dx.doi.org/10.5433/1679-0359.2021v42n2p695 http://hdl.handle.net/11449/205979 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 42, n. 2, p. 695-706, 2021. 1679-0359 1676-546X 10.5433/1679-0359.2021v42n2p695 2-s2.0-85101828030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina:Ciencias Agrarias |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
695-706 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128797684269056 |