Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2017/1502489 http://hdl.handle.net/11449/175953 |
Resumo: | High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O 2 group and the number of cells was assessed, with the 5% O 2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O 2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O 2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability. |
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spelling |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced EmbryosHigh oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O 2 group and the number of cells was assessed, with the 5% O 2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O 2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O 2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center of Natural and Human Sciences Universidade Federal Do ABCInstitute of Biomedical Sciences UniversidadeInstitute of Biosciences Campus Botucatu Department of Pharmacology Universidade Estadual Paulista (UNESP)School of Sciences and Languages Campus Assis Department of Biological Sciences Universidade Estadual Paulista (UNESP)Institute of Biosciences Campus Botucatu Department of Pharmacology Universidade Estadual Paulista (UNESP)School of Sciences and Languages Campus Assis Department of Biological Sciences Universidade Estadual Paulista (UNESP)Universidade Federal do ABC (UFABC)Sciences UniversidadeUniversidade Estadual Paulista (Unesp)Leite, Roberta FerreiraAnnes, KellyIspada, JessicaDe Lima, Camila BrunaDos Santos, Érika CristinaFontes, Patricia Kubo [UNESP]Nogueira, Marcelo Fábio Gouveia [UNESP]Milazzotto, Marcella Pecora2018-12-11T17:18:17Z2018-12-11T17:18:17Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2017/1502489Oxidative Medicine and Cellular Longevity, v. 2017.1942-09941942-0900http://hdl.handle.net/11449/17595310.1155/2017/15024892-s2.0-850426246942-s2.0-85042624694.pdf3734933152414412Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOxidative Medicine and Cellular Longevity1,5581,558info:eu-repo/semantics/openAccess2024-06-13T17:38:53Zoai:repositorio.unesp.br:11449/175953Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:07:48.383001Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
title |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
spellingShingle |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos Leite, Roberta Ferreira |
title_short |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
title_full |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
title_fullStr |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
title_full_unstemmed |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
title_sort |
Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on in Vitro Produced Embryos |
author |
Leite, Roberta Ferreira |
author_facet |
Leite, Roberta Ferreira Annes, Kelly Ispada, Jessica De Lima, Camila Bruna Dos Santos, Érika Cristina Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Milazzotto, Marcella Pecora |
author_role |
author |
author2 |
Annes, Kelly Ispada, Jessica De Lima, Camila Bruna Dos Santos, Érika Cristina Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Milazzotto, Marcella Pecora |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do ABC (UFABC) Sciences Universidade Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Leite, Roberta Ferreira Annes, Kelly Ispada, Jessica De Lima, Camila Bruna Dos Santos, Érika Cristina Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Milazzotto, Marcella Pecora |
description |
High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O 2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O 2 group and the number of cells was assessed, with the 5% O 2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O 2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O 2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:18:17Z 2018-12-11T17:18:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2017/1502489 Oxidative Medicine and Cellular Longevity, v. 2017. 1942-0994 1942-0900 http://hdl.handle.net/11449/175953 10.1155/2017/1502489 2-s2.0-85042624694 2-s2.0-85042624694.pdf 3734933152414412 |
url |
http://dx.doi.org/10.1155/2017/1502489 http://hdl.handle.net/11449/175953 |
identifier_str_mv |
Oxidative Medicine and Cellular Longevity, v. 2017. 1942-0994 1942-0900 10.1155/2017/1502489 2-s2.0-85042624694 2-s2.0-85042624694.pdf 3734933152414412 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oxidative Medicine and Cellular Longevity 1,558 1,558 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129394753929216 |