Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jnutbio.2020.108572 http://hdl.handle.net/11449/208332 |
Resumo: | We investigated whether combined long-term fructose and prednisolone intake would be more detrimental to the glucose homeostasis than if ingested separately. We also evaluated whether fish oil administration or interruption of treatments has any positive impact. For this, male adult Wistar rats ingested fructose (20%) (F) or prednisolone (12.5 µg/mL) (P) or both (FP) through drinking water for 12 weeks. A separate group of fructose and prednisolone-treated rats received fish oil treatment (1 g/kg) in the last 6 weeks. In another group, the treatment with fructose and prednisolone was interrupted after 12 weeks, and the animals were followed for more 12 weeks. Control groups ran in parallel (C). The F group had higher plasma TG (+42%) and visceral adiposity (+63%), whereas the P group had lower insulin sensitivity (−33%) and higher insulinemia (+200%). Only the the FP group developed these alterations combined with higher circulating uric acid (+126%), hepatic triacylglycerol content (+16.2-fold), lipid peroxidation (+173%) and lower catalase activity (−32%) that were associated with lower protein kinase B content and AMP-activated protein kinase (AMPK) phosphorylation in the liver, lower AMPK phosphorylation in the adipose tissue and higher beta-cell mass. Fish oil ingestion attenuated the elevation in circulating triacylglycerol and uric acid values, while the interruption of sugar and glucocorticoid intake reverted almost all modified parameters. In conclusion, long-term intake of fructose and prednisolone by male rats are more detrimental to glucose and lipid homeostasis than if ingested separately and the benefits of treatment interruption are broader than fish oil treatment. |
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Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administrationFructoseGlucocorticoidsMetabolic syndromeMetabolismSweetened beveragesWe investigated whether combined long-term fructose and prednisolone intake would be more detrimental to the glucose homeostasis than if ingested separately. We also evaluated whether fish oil administration or interruption of treatments has any positive impact. For this, male adult Wistar rats ingested fructose (20%) (F) or prednisolone (12.5 µg/mL) (P) or both (FP) through drinking water for 12 weeks. A separate group of fructose and prednisolone-treated rats received fish oil treatment (1 g/kg) in the last 6 weeks. In another group, the treatment with fructose and prednisolone was interrupted after 12 weeks, and the animals were followed for more 12 weeks. Control groups ran in parallel (C). The F group had higher plasma TG (+42%) and visceral adiposity (+63%), whereas the P group had lower insulin sensitivity (−33%) and higher insulinemia (+200%). Only the the FP group developed these alterations combined with higher circulating uric acid (+126%), hepatic triacylglycerol content (+16.2-fold), lipid peroxidation (+173%) and lower catalase activity (−32%) that were associated with lower protein kinase B content and AMP-activated protein kinase (AMPK) phosphorylation in the liver, lower AMPK phosphorylation in the adipose tissue and higher beta-cell mass. Fish oil ingestion attenuated the elevation in circulating triacylglycerol and uric acid values, while the interruption of sugar and glucocorticoid intake reverted almost all modified parameters. In conclusion, long-term intake of fructose and prednisolone by male rats are more detrimental to glucose and lipid homeostasis than if ingested separately and the benefits of treatment interruption are broader than fish oil treatment.Laboratory of Investigation in Chronic Diseases - LIDoC Department of Physiological Sciences Centre of Biological Sciences Federal University of Santa Catarina - UFSCMulticentre Graduate Program in Physiological Sciences Centre of Biological Sciences Federal University of Santa Catarina - UFSCGraduate Program in Nutrition Centre of Health Sciences Federal University of Santa Catarina - UFSCDepartment of Physiology and Pathology School of Dentistry São Paulo State University - UNESPDepartment of Physiology and Pathology School of Dentistry São Paulo State University - UNESPUniversidade Federal de Santa Catarina (UFSC)Universidade Estadual Paulista (Unesp)Santos, Cristiane dosda Silva, Julia SpanholBrunetta, Henver SimionatoChagas, Thays RodriguesZoccal, Daniel Breseghello [UNESP]Nunes, Everson AraújoRafacho, Alex2021-06-25T11:10:25Z2021-06-25T11:10:25Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jnutbio.2020.108572Journal of Nutritional Biochemistry, v. 90.1873-48470955-2863http://hdl.handle.net/11449/20833210.1016/j.jnutbio.2020.1085722-s2.0-85099571991Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Nutritional Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T19:02:02Zoai:repositorio.unesp.br:11449/208332Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
title |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
spellingShingle |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration Santos, Cristiane dos Fructose Glucocorticoids Metabolic syndrome Metabolism Sweetened beverages |
title_short |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
title_full |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
title_fullStr |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
title_full_unstemmed |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
title_sort |
Impact of combined long-term fructose and prednisolone intake on glucose and lipid homeostasis in rats: benefits of intake interruption or fish oil administration |
author |
Santos, Cristiane dos |
author_facet |
Santos, Cristiane dos da Silva, Julia Spanhol Brunetta, Henver Simionato Chagas, Thays Rodrigues Zoccal, Daniel Breseghello [UNESP] Nunes, Everson Araújo Rafacho, Alex |
author_role |
author |
author2 |
da Silva, Julia Spanhol Brunetta, Henver Simionato Chagas, Thays Rodrigues Zoccal, Daniel Breseghello [UNESP] Nunes, Everson Araújo Rafacho, Alex |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Santa Catarina (UFSC) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Santos, Cristiane dos da Silva, Julia Spanhol Brunetta, Henver Simionato Chagas, Thays Rodrigues Zoccal, Daniel Breseghello [UNESP] Nunes, Everson Araújo Rafacho, Alex |
dc.subject.por.fl_str_mv |
Fructose Glucocorticoids Metabolic syndrome Metabolism Sweetened beverages |
topic |
Fructose Glucocorticoids Metabolic syndrome Metabolism Sweetened beverages |
description |
We investigated whether combined long-term fructose and prednisolone intake would be more detrimental to the glucose homeostasis than if ingested separately. We also evaluated whether fish oil administration or interruption of treatments has any positive impact. For this, male adult Wistar rats ingested fructose (20%) (F) or prednisolone (12.5 µg/mL) (P) or both (FP) through drinking water for 12 weeks. A separate group of fructose and prednisolone-treated rats received fish oil treatment (1 g/kg) in the last 6 weeks. In another group, the treatment with fructose and prednisolone was interrupted after 12 weeks, and the animals were followed for more 12 weeks. Control groups ran in parallel (C). The F group had higher plasma TG (+42%) and visceral adiposity (+63%), whereas the P group had lower insulin sensitivity (−33%) and higher insulinemia (+200%). Only the the FP group developed these alterations combined with higher circulating uric acid (+126%), hepatic triacylglycerol content (+16.2-fold), lipid peroxidation (+173%) and lower catalase activity (−32%) that were associated with lower protein kinase B content and AMP-activated protein kinase (AMPK) phosphorylation in the liver, lower AMPK phosphorylation in the adipose tissue and higher beta-cell mass. Fish oil ingestion attenuated the elevation in circulating triacylglycerol and uric acid values, while the interruption of sugar and glucocorticoid intake reverted almost all modified parameters. In conclusion, long-term intake of fructose and prednisolone by male rats are more detrimental to glucose and lipid homeostasis than if ingested separately and the benefits of treatment interruption are broader than fish oil treatment. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:10:25Z 2021-06-25T11:10:25Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jnutbio.2020.108572 Journal of Nutritional Biochemistry, v. 90. 1873-4847 0955-2863 http://hdl.handle.net/11449/208332 10.1016/j.jnutbio.2020.108572 2-s2.0-85099571991 |
url |
http://dx.doi.org/10.1016/j.jnutbio.2020.108572 http://hdl.handle.net/11449/208332 |
identifier_str_mv |
Journal of Nutritional Biochemistry, v. 90. 1873-4847 0955-2863 10.1016/j.jnutbio.2020.108572 2-s2.0-85099571991 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Nutritional Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964574178344960 |