Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992006000400009 http://hdl.handle.net/11449/69208 |
Resumo: | Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption. |
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Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization processAntivenomCrotalus durissus terrificusHyperimmunizationIrradiationOvinesAnimaliaOvis ariesLaboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.Department of Tropical Diseases São Paulo State University UNESP, Botucatu, São PauloCenter for the Study of Venoms and Venomous Animals São Paulo State University UNESP, Botucatu, São PauloRadiobiology Supervision - Nuclear Energy Research Institute (IPEN/CNEN/SP), São PauloClinical Laboratory of Veterinary São Paulo State University UNESP, Botucatu, São PauloCentro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, 18618-000, Botucatu, SPDepartment of Tropical Diseases São Paulo State University UNESP, Botucatu, São PauloCenter for the Study of Venoms and Venomous Animals São Paulo State University UNESP, Botucatu, São PauloClinical Laboratory of Veterinary São Paulo State University UNESP, Botucatu, São PauloCentro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Instituto de Pesquisas Energéticas e Nucleares (IPEN)Ferreira Júnior, Rui Seabra [UNESP]Nascimento, N.Couto, R. [UNESP]Alves, J. B.Meira, D. A. [UNESP]Barraviera, Benedito [UNESP]2014-05-27T11:22:02Z2014-05-27T11:22:02Z2006-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article620-631application/pdfhttp://dx.doi.org/10.1590/S1678-91992006000400009Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006.1678-9199http://hdl.handle.net/11449/6920810.1590/S1678-91992006000400009S1678-91992006000400009WOS:0002462821000092-s2.0-338462943862-s2.0-33846294386.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2024-08-15T15:23:00Zoai:repositorio.unesp.br:11449/69208Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
title |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
spellingShingle |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process Ferreira Júnior, Rui Seabra [UNESP] Antivenom Crotalus durissus terrificus Hyperimmunization Irradiation Ovines Animalia Ovis aries |
title_short |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
title_full |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
title_fullStr |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
title_full_unstemmed |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
title_sort |
Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process |
author |
Ferreira Júnior, Rui Seabra [UNESP] |
author_facet |
Ferreira Júnior, Rui Seabra [UNESP] Nascimento, N. Couto, R. [UNESP] Alves, J. B. Meira, D. A. [UNESP] Barraviera, Benedito [UNESP] |
author_role |
author |
author2 |
Nascimento, N. Couto, R. [UNESP] Alves, J. B. Meira, D. A. [UNESP] Barraviera, Benedito [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Pesquisas Energéticas e Nucleares (IPEN) |
dc.contributor.author.fl_str_mv |
Ferreira Júnior, Rui Seabra [UNESP] Nascimento, N. Couto, R. [UNESP] Alves, J. B. Meira, D. A. [UNESP] Barraviera, Benedito [UNESP] |
dc.subject.por.fl_str_mv |
Antivenom Crotalus durissus terrificus Hyperimmunization Irradiation Ovines Animalia Ovis aries |
topic |
Antivenom Crotalus durissus terrificus Hyperimmunization Irradiation Ovines Animalia Ovis aries |
description |
Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-11-01 2014-05-27T11:22:02Z 2014-05-27T11:22:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992006000400009 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006. 1678-9199 http://hdl.handle.net/11449/69208 10.1590/S1678-91992006000400009 S1678-91992006000400009 WOS:000246282100009 2-s2.0-33846294386 2-s2.0-33846294386.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992006000400009 http://hdl.handle.net/11449/69208 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006. 1678-9199 10.1590/S1678-91992006000400009 S1678-91992006000400009 WOS:000246282100009 2-s2.0-33846294386 2-s2.0-33846294386.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
620-631 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128158104289280 |