Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process

Detalhes bibliográficos
Autor(a) principal: Ferreira Júnior, Rui Seabra [UNESP]
Data de Publicação: 2006
Outros Autores: Nascimento, N., Couto, R. [UNESP], Alves, J. B., Meira, D. A. [UNESP], Barraviera, Benedito [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992006000400009
http://hdl.handle.net/11449/69208
Resumo: Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.
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spelling Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization processAntivenomCrotalus durissus terrificusHyperimmunizationIrradiationOvinesAnimaliaOvis ariesLaboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.Department of Tropical Diseases São Paulo State University UNESP, Botucatu, São PauloCenter for the Study of Venoms and Venomous Animals São Paulo State University UNESP, Botucatu, São PauloRadiobiology Supervision - Nuclear Energy Research Institute (IPEN/CNEN/SP), São PauloClinical Laboratory of Veterinary São Paulo State University UNESP, Botucatu, São PauloCentro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, 18618-000, Botucatu, SPDepartment of Tropical Diseases São Paulo State University UNESP, Botucatu, São PauloCenter for the Study of Venoms and Venomous Animals São Paulo State University UNESP, Botucatu, São PauloClinical Laboratory of Veterinary São Paulo State University UNESP, Botucatu, São PauloCentro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, 18618-000, Botucatu, SPUniversidade Estadual Paulista (Unesp)Instituto de Pesquisas Energéticas e Nucleares (IPEN)Ferreira Júnior, Rui Seabra [UNESP]Nascimento, N.Couto, R. [UNESP]Alves, J. B.Meira, D. A. [UNESP]Barraviera, Benedito [UNESP]2014-05-27T11:22:02Z2014-05-27T11:22:02Z2006-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article620-631application/pdfhttp://dx.doi.org/10.1590/S1678-91992006000400009Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006.1678-9199http://hdl.handle.net/11449/6920810.1590/S1678-91992006000400009S1678-91992006000400009WOS:0002462821000092-s2.0-338462943862-s2.0-33846294386.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2024-08-15T15:23:00Zoai:repositorio.unesp.br:11449/69208Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
title Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
spellingShingle Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
Ferreira Júnior, Rui Seabra [UNESP]
Antivenom
Crotalus durissus terrificus
Hyperimmunization
Irradiation
Ovines
Animalia
Ovis aries
title_short Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
title_full Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
title_fullStr Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
title_full_unstemmed Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
title_sort Laboratory evaluation of young ovines inoculated with natural or 60Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process
author Ferreira Júnior, Rui Seabra [UNESP]
author_facet Ferreira Júnior, Rui Seabra [UNESP]
Nascimento, N.
Couto, R. [UNESP]
Alves, J. B.
Meira, D. A. [UNESP]
Barraviera, Benedito [UNESP]
author_role author
author2 Nascimento, N.
Couto, R. [UNESP]
Alves, J. B.
Meira, D. A. [UNESP]
Barraviera, Benedito [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.contributor.author.fl_str_mv Ferreira Júnior, Rui Seabra [UNESP]
Nascimento, N.
Couto, R. [UNESP]
Alves, J. B.
Meira, D. A. [UNESP]
Barraviera, Benedito [UNESP]
dc.subject.por.fl_str_mv Antivenom
Crotalus durissus terrificus
Hyperimmunization
Irradiation
Ovines
Animalia
Ovis aries
topic Antivenom
Crotalus durissus terrificus
Hyperimmunization
Irradiation
Ovines
Animalia
Ovis aries
description Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p<5%) among the groups tested. The hyperimmunization process was successfully accomplished with the production of specific antibodies against Crotalus durissus terrificus venom. Results bring a new possibility of utilizing ovines in the commercial production of anticrotalic serum, which may be used to treat human and animal envenomation. Its production cost may be reduced by subsequent use of hyperimmunized sheep for human consumption.
publishDate 2006
dc.date.none.fl_str_mv 2006-11-01
2014-05-27T11:22:02Z
2014-05-27T11:22:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992006000400009
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006.
1678-9199
http://hdl.handle.net/11449/69208
10.1590/S1678-91992006000400009
S1678-91992006000400009
WOS:000246282100009
2-s2.0-33846294386
2-s2.0-33846294386.pdf
url http://dx.doi.org/10.1590/S1678-91992006000400009
http://hdl.handle.net/11449/69208
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 12, n. 4, p. 620-631, 2006.
1678-9199
10.1590/S1678-91992006000400009
S1678-91992006000400009
WOS:000246282100009
2-s2.0-33846294386
2-s2.0-33846294386.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
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0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 620-631
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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