Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/149226 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/23-11-2016/000874656.pdf |
Resumo: | Studies have been conducted in order to prevent biofilm formation on biomaterials or seek alternative therapies for the treatment of diseases caused by biofilm installation. As an alternative therapy, this study evaluated (1) the cytotoxicity of photodynamic therapy (PDT) by the use of co-cultured human keratinocytes with Candida albicans (Ca). In relation to prevention of biofilm formation, (2) the cytotoxicity was evaluated nanoparticles (NP) silver tungstate (Ag2WO4) and silver molybdate (Ag2MoO4), in solution and as a biomaterial coating. In study 1, the co-culture was performed using a Transwell membrane, and cells in which micro-organisms grown separately for 24 h, and were in contact for a further 24h.After this period, PDT was performed using curcumin as a photosensitizer. The following conditions were tested: P+ L+; P- L+; P+ L; P- L. In addition, as a negative control, three membranes of each plate were assigned only to the growth of microorganisms and another three wells for cell growth separately with respective means. Cell proliferation was evaluated by testing the Alamar Blue, MTT, XTT, and CFU. For the study 2, the NP were synthesized and characterized by scanning electronic microscopy and X-ray diffraction. Since the making of the NP, they were made solutions and the titanium coating (Ti), zirconium (Zi), acrylic resin (RA) and silicon (Si). To perform the cytotoxicity assay, 100 µL of suspension composed of 1.5 x 104 cells / mL (HaCaT) were placed in each compartment of a plate with 96 wells, incubated in an incubator with 5% CO2 at 37 ° C for 24 hours. After this incubation period, the culture medium was discarded, remaining adhered cells at the bottom of the wells. 100 µL of culture medium containing the nanoparticles in solution and the extracts were placed on each plate hole. The plate was incubated for another 24 hours. Cell proliferation was assessed using the Alamar ... (Complete abstract electronic access below) |
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Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilmeBiocompatibility of substances used for biofilm prevention or eliminationTeste de materiaisPlaca dentáriaFotoquimioterapiaNanopartículasCelulas - Cultura e meios de culturaMaterials testingBiofilmsPhotochemotherapyNanoparticlesStudies have been conducted in order to prevent biofilm formation on biomaterials or seek alternative therapies for the treatment of diseases caused by biofilm installation. As an alternative therapy, this study evaluated (1) the cytotoxicity of photodynamic therapy (PDT) by the use of co-cultured human keratinocytes with Candida albicans (Ca). In relation to prevention of biofilm formation, (2) the cytotoxicity was evaluated nanoparticles (NP) silver tungstate (Ag2WO4) and silver molybdate (Ag2MoO4), in solution and as a biomaterial coating. In study 1, the co-culture was performed using a Transwell membrane, and cells in which micro-organisms grown separately for 24 h, and were in contact for a further 24h.After this period, PDT was performed using curcumin as a photosensitizer. The following conditions were tested: P+ L+; P- L+; P+ L; P- L. In addition, as a negative control, three membranes of each plate were assigned only to the growth of microorganisms and another three wells for cell growth separately with respective means. Cell proliferation was evaluated by testing the Alamar Blue, MTT, XTT, and CFU. For the study 2, the NP were synthesized and characterized by scanning electronic microscopy and X-ray diffraction. Since the making of the NP, they were made solutions and the titanium coating (Ti), zirconium (Zi), acrylic resin (RA) and silicon (Si). To perform the cytotoxicity assay, 100 µL of suspension composed of 1.5 x 104 cells / mL (HaCaT) were placed in each compartment of a plate with 96 wells, incubated in an incubator with 5% CO2 at 37 ° C for 24 hours. After this incubation period, the culture medium was discarded, remaining adhered cells at the bottom of the wells. 100 µL of culture medium containing the nanoparticles in solution and the extracts were placed on each plate hole. The plate was incubated for another 24 hours. Cell proliferation was assessed using the Alamar ... (Complete abstract electronic access below)Estudos têm sido conduzidos com o intuito de prevenir a formação do biofilme em biomateriais ou buscar terapias alternativas para o tratamento de doenças decorrentes da instalação do biofilme. Como terapia alternativa, este estudo avaliou (1) a citotoxicidade da terapia fotodinâmica (PDT), através da utilização de queratinócitos humanos co-cultivados com Candida albicans. Em relação à prevenção da formação do biofilme, (2) foi avaliada a citotoxicidade de nanopartículas (NP) de tungstato de prata (Ag2WO4) e de molibdato de prata (Ag2MoO4), em solução e como revestimento de biomateriais. No estudo 1, a co-cultura foi realizada utilizando uma membrana Transwell, em que células e micro-organismos cresceram separadamente durante 24h, e ficaram em contato por mais 24h. Após este período, a PDT foi realizada utilizando-se a curcumina como fotossensibilizador. As seguintes condições foram testadas: P+ L+; P- L+; P+ L; P- L. Além disso, como controle negativo, três membranas de cada placa foram destinadas apenas ao crescimento de microorganismos e outros três poços para o crescimento de células separadamente, com seus respectivos meios. A proliferação celular, foi avaliada através dos testes Alamar Blue, MTT, XTT e UFC. Para o estudo 2, as NP foram sintetizadas e caracterizadas através de Microscopia Eletrônica de Varredura e Difração de Raios X. A partir da confecção das NP, foram feitas as soluções e o revestimento de titânio (Ti), zircônia (Zi), resina acrílica (RA) e silicone (Si). Para a realização do teste de citotoxicidade, 100 µL da suspensão composta por 1,5 x 104 células/ml (HaCat) foram colocados em cada compartimento de uma placa com 96 orifícios, incubada em estufa com 5% de CO2, a 37ºC por 24 horas. Após tal período de incubação, o meio de cultura foi desprezado, permanecendo as células aderidas no fundo da placa...(Resumo completo, clicar acesso eletrônico abaixo)Universidade Estadual Paulista (Unesp)Jorge, Janaina Habib [UNESP]Universidade Estadual Paulista (Unesp)Pellissari, Cláudia Viviane Guimarães [UNESP]2017-03-14T14:10:06Z2017-03-14T14:10:06Z2015-09-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis144 f.application/pdfPELLISSARI, Cláudia Viviane Guimarães. Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme. 2015. 144 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015.http://hdl.handle.net/11449/149226000874656http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/23-11-2016/000874656.pdf33004030082P3Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-21T06:27:30Zoai:repositorio.unesp.br:11449/149226Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:37:35.003839Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme Biocompatibility of substances used for biofilm prevention or elimination |
title |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
spellingShingle |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme Pellissari, Cláudia Viviane Guimarães [UNESP] Teste de materiais Placa dentária Fotoquimioterapia Nanopartículas Celulas - Cultura e meios de cultura Materials testing Biofilms Photochemotherapy Nanoparticles |
title_short |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
title_full |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
title_fullStr |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
title_full_unstemmed |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
title_sort |
Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme |
author |
Pellissari, Cláudia Viviane Guimarães [UNESP] |
author_facet |
Pellissari, Cláudia Viviane Guimarães [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Jorge, Janaina Habib [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pellissari, Cláudia Viviane Guimarães [UNESP] |
dc.subject.por.fl_str_mv |
Teste de materiais Placa dentária Fotoquimioterapia Nanopartículas Celulas - Cultura e meios de cultura Materials testing Biofilms Photochemotherapy Nanoparticles |
topic |
Teste de materiais Placa dentária Fotoquimioterapia Nanopartículas Celulas - Cultura e meios de cultura Materials testing Biofilms Photochemotherapy Nanoparticles |
description |
Studies have been conducted in order to prevent biofilm formation on biomaterials or seek alternative therapies for the treatment of diseases caused by biofilm installation. As an alternative therapy, this study evaluated (1) the cytotoxicity of photodynamic therapy (PDT) by the use of co-cultured human keratinocytes with Candida albicans (Ca). In relation to prevention of biofilm formation, (2) the cytotoxicity was evaluated nanoparticles (NP) silver tungstate (Ag2WO4) and silver molybdate (Ag2MoO4), in solution and as a biomaterial coating. In study 1, the co-culture was performed using a Transwell membrane, and cells in which micro-organisms grown separately for 24 h, and were in contact for a further 24h.After this period, PDT was performed using curcumin as a photosensitizer. The following conditions were tested: P+ L+; P- L+; P+ L; P- L. In addition, as a negative control, three membranes of each plate were assigned only to the growth of microorganisms and another three wells for cell growth separately with respective means. Cell proliferation was evaluated by testing the Alamar Blue, MTT, XTT, and CFU. For the study 2, the NP were synthesized and characterized by scanning electronic microscopy and X-ray diffraction. Since the making of the NP, they were made solutions and the titanium coating (Ti), zirconium (Zi), acrylic resin (RA) and silicon (Si). To perform the cytotoxicity assay, 100 µL of suspension composed of 1.5 x 104 cells / mL (HaCaT) were placed in each compartment of a plate with 96 wells, incubated in an incubator with 5% CO2 at 37 ° C for 24 hours. After this incubation period, the culture medium was discarded, remaining adhered cells at the bottom of the wells. 100 µL of culture medium containing the nanoparticles in solution and the extracts were placed on each plate hole. The plate was incubated for another 24 hours. Cell proliferation was assessed using the Alamar ... (Complete abstract electronic access below) |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-11 2017-03-14T14:10:06Z 2017-03-14T14:10:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PELLISSARI, Cláudia Viviane Guimarães. Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme. 2015. 144 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015. http://hdl.handle.net/11449/149226 000874656 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/23-11-2016/000874656.pdf 33004030082P3 |
identifier_str_mv |
PELLISSARI, Cláudia Viviane Guimarães. Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme. 2015. 144 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia (FOAR), 2015. 000874656 33004030082P3 |
url |
http://hdl.handle.net/11449/149226 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/23-11-2016/000874656.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
144 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129537598291968 |