Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants
Main Author: | |
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Publication Date: | 2022 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1007/s42770-022-00824-z http://hdl.handle.net/11449/237876 |
Summary: | Inoculants with beneficial microorganisms comprise both selected strains and carriers that ensure a favorable microenvironment for cell survival and stability. Formulations of inoculants using synthetic polymers as carriers are common. However, only a few studies are available in the literature regarding the formulation of inoculants using natural biomolecules as carriers. Exopolysaccharides (EPS) are biomolecules produced by a vast array of microbial species, including symbiotic nitrogen-fixing bacteria, commonly known as rhizobia. EPS perform several functions, such as the protection against the deleterious effects of diverse environmental soil stresses. Two Rhizobium tropici strains and one Paraburkholderia strain were selected after semiquantitative analysis by scanning electron microscopy (SEM) of their EPS production in liquid YMA medium. Their EPS were characterized through a series of analytical techniques, aiming at their use in the formulation of plant inoculants. In addition, the effect of the carbon source on EPS yield was evaluated. Multi-stage fragmentation analysis showed the presence of xylose, glucose, galactose, galacturonic acid, and glucuronic acid in EPS chemical composition, which was confirmed by FT-IR spectra and C-13 NMR spectroscopy. Thermal stability (thermogravimetric) was close to 270 degrees C and viscosity ranged from 120 to 1053.3 mPa.s. Surface morphology (SEM) was rough and irregular, with a cross-linked spongy matrix, which, together with the hydrophilic functional groups, confers water holding capacity. The present study showed that the three EPS have potential as microorganism carriers for formulation of microbial inoculants to be applied in plants. |
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Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculantsBacterial exopolysaccharideWater holdingCellular protectionInoculant carrierParaburkholderiaRhizobium tropiciInoculants with beneficial microorganisms comprise both selected strains and carriers that ensure a favorable microenvironment for cell survival and stability. Formulations of inoculants using synthetic polymers as carriers are common. However, only a few studies are available in the literature regarding the formulation of inoculants using natural biomolecules as carriers. Exopolysaccharides (EPS) are biomolecules produced by a vast array of microbial species, including symbiotic nitrogen-fixing bacteria, commonly known as rhizobia. EPS perform several functions, such as the protection against the deleterious effects of diverse environmental soil stresses. Two Rhizobium tropici strains and one Paraburkholderia strain were selected after semiquantitative analysis by scanning electron microscopy (SEM) of their EPS production in liquid YMA medium. Their EPS were characterized through a series of analytical techniques, aiming at their use in the formulation of plant inoculants. In addition, the effect of the carbon source on EPS yield was evaluated. Multi-stage fragmentation analysis showed the presence of xylose, glucose, galactose, galacturonic acid, and glucuronic acid in EPS chemical composition, which was confirmed by FT-IR spectra and C-13 NMR spectroscopy. Thermal stability (thermogravimetric) was close to 270 degrees C and viscosity ranged from 120 to 1053.3 mPa.s. Surface morphology (SEM) was rough and irregular, with a cross-linked spongy matrix, which, together with the hydrophilic functional groups, confers water holding capacity. The present study showed that the three EPS have potential as microorganism carriers for formulation of microbial inoculants to be applied in plants.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Program for the Qualification of Public Servants of IFMA (PROQUALIS)Univ Fed Lavras UFLA, Dept Ciencia Solo, Caixa Postal 3037, BR-37200900 Lavras, MG, BrazilIFMA, Lab Microbiol Solo & Biotecnol DDE, Campus Sao Luis Maracana, BR-65095460 Sao Luis, Maranhao, BrazilUniv Fed Lavras, Caixa Postal 3037, BR-37200900 Lavras, MG, BrazilUNESP, Inst Biociencias, Campus Litoral Paulista, BR-11330900 Sao Vincente, SP, BrazilUniv Fed Maranhao, Dept Quim, Campus Univ Dom Delgado 1966, BR-65080040 Sao Luis, Maranhao, BrazilUniv Fed Lavras, Dept Ciencia Solo, Campus Univ 3037, BR-37200900 Lavras, MG, BrazilUNESP, Inst Biociencias, Campus Litoral Paulista, BR-11330900 Sao Vincente, SP, BrazilCAPES: CAPES/PROEX AUXPE 593/2018CNPq: 304527/2016-5CNPq: 431504/2016-4FAPEMIG: CAG-RED-00330-16SpringerUniversidade Federal de Lavras (UFLA)IFMAUniversidade Estadual Paulista (UNESP)Univ Fed MaranhaoFarias, Thiago PalharesCastro, Elisa de MeloTangerina, Marcelo Marucci Pereira[UNESP]Rocha, Claudia Quintino daBezerra, Cicero Wellington BritoMoreira, Fatima Maria de Souza2022-11-30T13:47:21Z2022-11-30T13:47:21Z2022-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1007/s42770-022-00824-zBrazilian Journal Of Microbiology. New York: Springer, 14 p., 2022.1517-8382http://hdl.handle.net/11449/23787610.1007/s42770-022-00824-zWOS:000854663800001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2022-11-30T13:47:21Zoai:repositorio.unesp.br:11449/237876Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-11-30T13:47:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
title |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
spellingShingle |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants Farias, Thiago Palhares Bacterial exopolysaccharide Water holding Cellular protection Inoculant carrier Paraburkholderia Rhizobium tropici |
title_short |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
title_full |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
title_fullStr |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
title_full_unstemmed |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
title_sort |
Rhizobia exopolysaccharides: promising biopolymers for use in the formulation of plant inoculants |
author |
Farias, Thiago Palhares |
author_facet |
Farias, Thiago Palhares Castro, Elisa de Melo Tangerina, Marcelo Marucci Pereira[UNESP] Rocha, Claudia Quintino da Bezerra, Cicero Wellington Brito Moreira, Fatima Maria de Souza |
author_role |
author |
author2 |
Castro, Elisa de Melo Tangerina, Marcelo Marucci Pereira[UNESP] Rocha, Claudia Quintino da Bezerra, Cicero Wellington Brito Moreira, Fatima Maria de Souza |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras (UFLA) IFMA Universidade Estadual Paulista (UNESP) Univ Fed Maranhao |
dc.contributor.author.fl_str_mv |
Farias, Thiago Palhares Castro, Elisa de Melo Tangerina, Marcelo Marucci Pereira[UNESP] Rocha, Claudia Quintino da Bezerra, Cicero Wellington Brito Moreira, Fatima Maria de Souza |
dc.subject.por.fl_str_mv |
Bacterial exopolysaccharide Water holding Cellular protection Inoculant carrier Paraburkholderia Rhizobium tropici |
topic |
Bacterial exopolysaccharide Water holding Cellular protection Inoculant carrier Paraburkholderia Rhizobium tropici |
description |
Inoculants with beneficial microorganisms comprise both selected strains and carriers that ensure a favorable microenvironment for cell survival and stability. Formulations of inoculants using synthetic polymers as carriers are common. However, only a few studies are available in the literature regarding the formulation of inoculants using natural biomolecules as carriers. Exopolysaccharides (EPS) are biomolecules produced by a vast array of microbial species, including symbiotic nitrogen-fixing bacteria, commonly known as rhizobia. EPS perform several functions, such as the protection against the deleterious effects of diverse environmental soil stresses. Two Rhizobium tropici strains and one Paraburkholderia strain were selected after semiquantitative analysis by scanning electron microscopy (SEM) of their EPS production in liquid YMA medium. Their EPS were characterized through a series of analytical techniques, aiming at their use in the formulation of plant inoculants. In addition, the effect of the carbon source on EPS yield was evaluated. Multi-stage fragmentation analysis showed the presence of xylose, glucose, galactose, galacturonic acid, and glucuronic acid in EPS chemical composition, which was confirmed by FT-IR spectra and C-13 NMR spectroscopy. Thermal stability (thermogravimetric) was close to 270 degrees C and viscosity ranged from 120 to 1053.3 mPa.s. Surface morphology (SEM) was rough and irregular, with a cross-linked spongy matrix, which, together with the hydrophilic functional groups, confers water holding capacity. The present study showed that the three EPS have potential as microorganism carriers for formulation of microbial inoculants to be applied in plants. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:47:21Z 2022-11-30T13:47:21Z 2022-09-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s42770-022-00824-z Brazilian Journal Of Microbiology. New York: Springer, 14 p., 2022. 1517-8382 http://hdl.handle.net/11449/237876 10.1007/s42770-022-00824-z WOS:000854663800001 |
url |
http://dx.doi.org/10.1007/s42770-022-00824-z http://hdl.handle.net/11449/237876 |
identifier_str_mv |
Brazilian Journal Of Microbiology. New York: Springer, 14 p., 2022. 1517-8382 10.1007/s42770-022-00824-z WOS:000854663800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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