Characterization of a monoclonal antibody recognizing mast cells
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/195668 |
Resumo: | Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not, labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells. |
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Characterization of a monoclonal antibody recognizing mast cellsmast cellsheterogeneityimmunohistochemistryconnective tissueintestineImmunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not, labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.UNESP,SCH DENT SAO JOSE DOS CAMPOS,SAO PAULO,BRAZILUNIV TORONTO,FAC MED,DEPT ANAT & CELL BIOL,TORONTO,ON,CANADAUNESP,SCH DENT SAO JOSE DOS CAMPOS,SAO PAULO,BRAZILF HernandezUniversidade Estadual Paulista (Unesp)UNIV TORONTONogueira, T. D.Aubin, J. E.2020-12-10T17:59:39Z2020-12-10T17:59:39Z1997-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article919-923Histology And Histopathology. Murcia: F Hernandez, v. 12, n. 4, p. 919-923, 1997.0213-3911http://hdl.handle.net/11449/195668WOS:A1997XX08600004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHistology And Histopathologyinfo:eu-repo/semantics/openAccess2021-10-23T10:18:36Zoai:repositorio.unesp.br:11449/195668Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of a monoclonal antibody recognizing mast cells |
title |
Characterization of a monoclonal antibody recognizing mast cells |
spellingShingle |
Characterization of a monoclonal antibody recognizing mast cells Nogueira, T. D. mast cells heterogeneity immunohistochemistry connective tissue intestine |
title_short |
Characterization of a monoclonal antibody recognizing mast cells |
title_full |
Characterization of a monoclonal antibody recognizing mast cells |
title_fullStr |
Characterization of a monoclonal antibody recognizing mast cells |
title_full_unstemmed |
Characterization of a monoclonal antibody recognizing mast cells |
title_sort |
Characterization of a monoclonal antibody recognizing mast cells |
author |
Nogueira, T. D. |
author_facet |
Nogueira, T. D. Aubin, J. E. |
author_role |
author |
author2 |
Aubin, J. E. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIV TORONTO |
dc.contributor.author.fl_str_mv |
Nogueira, T. D. Aubin, J. E. |
dc.subject.por.fl_str_mv |
mast cells heterogeneity immunohistochemistry connective tissue intestine |
topic |
mast cells heterogeneity immunohistochemistry connective tissue intestine |
description |
Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not, labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-10-01 2020-12-10T17:59:39Z 2020-12-10T17:59:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 12, n. 4, p. 919-923, 1997. 0213-3911 http://hdl.handle.net/11449/195668 WOS:A1997XX08600004 |
identifier_str_mv |
Histology And Histopathology. Murcia: F Hernandez, v. 12, n. 4, p. 919-923, 1997. 0213-3911 WOS:A1997XX08600004 |
url |
http://hdl.handle.net/11449/195668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Histology And Histopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
919-923 |
dc.publisher.none.fl_str_mv |
F Hernandez |
publisher.none.fl_str_mv |
F Hernandez |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046358914957312 |