Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jsbmb.2016.06.001 http://hdl.handle.net/11449/173860 |
Resumo: | 4-vinilcyclohexene diepoxide (4-VCD) causes premature ovarian failure and may result in estrogen deficiency, characterizing the transition to estropause in rodents (equivalent to menopause in women). Estropause/menopause is associated with metabolic derangements such as glucose intolerance and insulin resistance. Glucocorticoids (GCs) are known to exert diabetogenic effects. Thus, we aimed to investigate whether rats with premature ovarian failure are more prone to the diabetogenic effects of GC. For this, immature female rats received daily injections of 4-VCD [160 mg/kg body weight (b.w.), intraperitoneally (i.p.)] for 15 consecutive days, whereas control rats received vehicle. After 168 days of the completion of 4-VCD administration, rats were divided into 4 groups: CTL—received daily injections of saline (1 mL/kg, b.w., i.p.) for 5 days; DEX—received daily injections of dexamethasone (1 mg/kg, b.w., i.p.) for 5 days; VCD—treated as CTL group; VCD + DEX—treated as DEX group. Experiments and euthanasia occurred one day after the last dexamethasone injection. 4-VCD-treated rats exhibited ovary hypotrophy and reduced number of preantral follicles (p < 0.05). Premature ovarian failure had no impact on the body weight gain or food intake, but both were reduced by the effects of dexamethasone. The increase in blood glucose, plasma insulin and triacylglycerol levels as well as the reduction in insulin sensitivity caused by dexamethasone treatment was not exacerbated in the VCD + DEX group of rats. Premature ovarian failure did change neither the hepatic content of glycogen and triacylglycerol nor the glycerol release from perigonadal adipose tissue. Glucose intolerance was observed in the VCD group after an ipGTT (p < 0.05), but not after an oral glucose challenge. Glucose intolerance and compensatory pancreatic β-cell mass caused by GC were not modified by ovarian failure in the VCD + DEX group. We conclude that reduced ovarian function has no major implications on the diabetogenic effects promoted by GC treatment, indicating that other factors related to aging may make rats more vulnerable to GC side effects on glucose metabolism. |
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Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatmentAgingFemaleMetabolismOvarian failureSenescenceSteroid hormones4-vinilcyclohexene diepoxide (4-VCD) causes premature ovarian failure and may result in estrogen deficiency, characterizing the transition to estropause in rodents (equivalent to menopause in women). Estropause/menopause is associated with metabolic derangements such as glucose intolerance and insulin resistance. Glucocorticoids (GCs) are known to exert diabetogenic effects. Thus, we aimed to investigate whether rats with premature ovarian failure are more prone to the diabetogenic effects of GC. For this, immature female rats received daily injections of 4-VCD [160 mg/kg body weight (b.w.), intraperitoneally (i.p.)] for 15 consecutive days, whereas control rats received vehicle. After 168 days of the completion of 4-VCD administration, rats were divided into 4 groups: CTL—received daily injections of saline (1 mL/kg, b.w., i.p.) for 5 days; DEX—received daily injections of dexamethasone (1 mg/kg, b.w., i.p.) for 5 days; VCD—treated as CTL group; VCD + DEX—treated as DEX group. Experiments and euthanasia occurred one day after the last dexamethasone injection. 4-VCD-treated rats exhibited ovary hypotrophy and reduced number of preantral follicles (p < 0.05). Premature ovarian failure had no impact on the body weight gain or food intake, but both were reduced by the effects of dexamethasone. The increase in blood glucose, plasma insulin and triacylglycerol levels as well as the reduction in insulin sensitivity caused by dexamethasone treatment was not exacerbated in the VCD + DEX group of rats. Premature ovarian failure did change neither the hepatic content of glycogen and triacylglycerol nor the glycerol release from perigonadal adipose tissue. Glucose intolerance was observed in the VCD group after an ipGTT (p < 0.05), but not after an oral glucose challenge. Glucose intolerance and compensatory pancreatic β-cell mass caused by GC were not modified by ovarian failure in the VCD + DEX group. We conclude that reduced ovarian function has no major implications on the diabetogenic effects promoted by GC treatment, indicating that other factors related to aging may make rats more vulnerable to GC side effects on glucose metabolism.Department of Physiological Sciences and Multicenter Graduate Program in Physiological Sciences Center of Biological Sciences Federal University of Santa Catarina—UFSCDepartment of Biology Institute of Biosciences Letters and Exact Sciences Univ. Estadual Paulista—IBILCE/UNESPDepartment of Morphology Center of Biological Sciences School of Dentistry of Ribeirão Preto São Paulo University—USPDepartment of Biology Institute of Biosciences Letters and Exact Sciences Univ. Estadual Paulista—IBILCE/UNESPUniversidade Federal de Santa Catarina (UFSC)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Battiston, Francielle Garghettidos Santos, CristianeBarbosa, Amanda MarreiroSehnem, SibeleLeonel, Ellen Cristina Rivas [UNESP]Taboga, Sebastião Roberto [UNESP]Anselmo-Franci, Janete A.Lima, Fernanda BarbosaRafacho, Alex2018-12-11T17:08:05Z2018-12-11T17:08:05Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article170-181application/pdfhttp://dx.doi.org/10.1016/j.jsbmb.2016.06.0012-s2.0-85000542303.pdfJournal of Steroid Biochemistry and Molecular Biology, v. 165, p. 170-181.1879-12200960-0760http://hdl.handle.net/11449/17386010.1016/j.jsbmb.2016.06.0012-s2.0-85000542303Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Steroid Biochemistry and Molecular Biology1,431info:eu-repo/semantics/openAccess2023-12-30T06:15:47Zoai:repositorio.unesp.br:11449/173860Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:39:32.614162Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
title |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
spellingShingle |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment Battiston, Francielle Garghetti Aging Female Metabolism Ovarian failure Senescence Steroid hormones |
title_short |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
title_full |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
title_fullStr |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
title_full_unstemmed |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
title_sort |
Glucose homeostasis in rats treated with 4-vinylcyclohexene diepoxide is not worsened by dexamethasone treatment |
author |
Battiston, Francielle Garghetti |
author_facet |
Battiston, Francielle Garghetti dos Santos, Cristiane Barbosa, Amanda Marreiro Sehnem, Sibele Leonel, Ellen Cristina Rivas [UNESP] Taboga, Sebastião Roberto [UNESP] Anselmo-Franci, Janete A. Lima, Fernanda Barbosa Rafacho, Alex |
author_role |
author |
author2 |
dos Santos, Cristiane Barbosa, Amanda Marreiro Sehnem, Sibele Leonel, Ellen Cristina Rivas [UNESP] Taboga, Sebastião Roberto [UNESP] Anselmo-Franci, Janete A. Lima, Fernanda Barbosa Rafacho, Alex |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Santa Catarina (UFSC) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Battiston, Francielle Garghetti dos Santos, Cristiane Barbosa, Amanda Marreiro Sehnem, Sibele Leonel, Ellen Cristina Rivas [UNESP] Taboga, Sebastião Roberto [UNESP] Anselmo-Franci, Janete A. Lima, Fernanda Barbosa Rafacho, Alex |
dc.subject.por.fl_str_mv |
Aging Female Metabolism Ovarian failure Senescence Steroid hormones |
topic |
Aging Female Metabolism Ovarian failure Senescence Steroid hormones |
description |
4-vinilcyclohexene diepoxide (4-VCD) causes premature ovarian failure and may result in estrogen deficiency, characterizing the transition to estropause in rodents (equivalent to menopause in women). Estropause/menopause is associated with metabolic derangements such as glucose intolerance and insulin resistance. Glucocorticoids (GCs) are known to exert diabetogenic effects. Thus, we aimed to investigate whether rats with premature ovarian failure are more prone to the diabetogenic effects of GC. For this, immature female rats received daily injections of 4-VCD [160 mg/kg body weight (b.w.), intraperitoneally (i.p.)] for 15 consecutive days, whereas control rats received vehicle. After 168 days of the completion of 4-VCD administration, rats were divided into 4 groups: CTL—received daily injections of saline (1 mL/kg, b.w., i.p.) for 5 days; DEX—received daily injections of dexamethasone (1 mg/kg, b.w., i.p.) for 5 days; VCD—treated as CTL group; VCD + DEX—treated as DEX group. Experiments and euthanasia occurred one day after the last dexamethasone injection. 4-VCD-treated rats exhibited ovary hypotrophy and reduced number of preantral follicles (p < 0.05). Premature ovarian failure had no impact on the body weight gain or food intake, but both were reduced by the effects of dexamethasone. The increase in blood glucose, plasma insulin and triacylglycerol levels as well as the reduction in insulin sensitivity caused by dexamethasone treatment was not exacerbated in the VCD + DEX group of rats. Premature ovarian failure did change neither the hepatic content of glycogen and triacylglycerol nor the glycerol release from perigonadal adipose tissue. Glucose intolerance was observed in the VCD group after an ipGTT (p < 0.05), but not after an oral glucose challenge. Glucose intolerance and compensatory pancreatic β-cell mass caused by GC were not modified by ovarian failure in the VCD + DEX group. We conclude that reduced ovarian function has no major implications on the diabetogenic effects promoted by GC treatment, indicating that other factors related to aging may make rats more vulnerable to GC side effects on glucose metabolism. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:08:05Z 2018-12-11T17:08:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jsbmb.2016.06.001 2-s2.0-85000542303.pdf Journal of Steroid Biochemistry and Molecular Biology, v. 165, p. 170-181. 1879-1220 0960-0760 http://hdl.handle.net/11449/173860 10.1016/j.jsbmb.2016.06.001 2-s2.0-85000542303 |
url |
http://dx.doi.org/10.1016/j.jsbmb.2016.06.001 http://hdl.handle.net/11449/173860 |
identifier_str_mv |
2-s2.0-85000542303.pdf Journal of Steroid Biochemistry and Molecular Biology, v. 165, p. 170-181. 1879-1220 0960-0760 10.1016/j.jsbmb.2016.06.001 2-s2.0-85000542303 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Steroid Biochemistry and Molecular Biology 1,431 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
170-181 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129344731611136 |