Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2018.1425402 http://hdl.handle.net/11449/179538 |
Resumo: | This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms. |
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Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasisBiofilmCandida albicansLactobacillusmycologypathogenesisThis study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.Brown UniversityFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP–Univ Estadual PaulistaDivision of Infectious Diseases Rhode Island Hospital Alpert Medical School of Brown UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP–Univ Estadual PaulistaFAPESP: 2013/25181-8FAPESP: 2013/26820-4FAPESP: 2015/09770-9Universidade Estadual Paulista (Unesp)Alpert Medical School of Brown UniversityRossoni, Rodnei Dennis [UNESP]de Barros, Patrícia Pimentel [UNESP]de Alvarenga, Janaina Araújo [UNESP]Ribeiro, Felipe de Camargo [UNESP]Velloso, Marisol dos Santos [UNESP]Fuchs, Beth BurgwynMylonakis, EleftheriosJorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]2018-12-11T17:35:35Z2018-12-11T17:35:35Z2018-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article212-225application/pdfhttp://dx.doi.org/10.1080/08927014.2018.1425402Biofouling, v. 34, n. 2, p. 212-225, 2018.1029-24540892-7014http://hdl.handle.net/11449/17953810.1080/08927014.2018.14254022-s2.0-850412164672-s2.0-85041216467.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2023-11-26T06:10:34Zoai:repositorio.unesp.br:11449/179538Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:45:36.032336Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
title |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
spellingShingle |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis Rossoni, Rodnei Dennis [UNESP] Biofilm Candida albicans Lactobacillus mycology pathogenesis |
title_short |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
title_full |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
title_fullStr |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
title_full_unstemmed |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
title_sort |
Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis |
author |
Rossoni, Rodnei Dennis [UNESP] |
author_facet |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] de Alvarenga, Janaina Araújo [UNESP] Ribeiro, Felipe de Camargo [UNESP] Velloso, Marisol dos Santos [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author_role |
author |
author2 |
de Barros, Patrícia Pimentel [UNESP] de Alvarenga, Janaina Araújo [UNESP] Ribeiro, Felipe de Camargo [UNESP] Velloso, Marisol dos Santos [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Alpert Medical School of Brown University |
dc.contributor.author.fl_str_mv |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] de Alvarenga, Janaina Araújo [UNESP] Ribeiro, Felipe de Camargo [UNESP] Velloso, Marisol dos Santos [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Candida albicans Lactobacillus mycology pathogenesis |
topic |
Biofilm Candida albicans Lactobacillus mycology pathogenesis |
description |
This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:35Z 2018-12-11T17:35:35Z 2018-02-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2018.1425402 Biofouling, v. 34, n. 2, p. 212-225, 2018. 1029-2454 0892-7014 http://hdl.handle.net/11449/179538 10.1080/08927014.2018.1425402 2-s2.0-85041216467 2-s2.0-85041216467.pdf |
url |
http://dx.doi.org/10.1080/08927014.2018.1425402 http://hdl.handle.net/11449/179538 |
identifier_str_mv |
Biofouling, v. 34, n. 2, p. 212-225, 2018. 1029-2454 0892-7014 10.1080/08927014.2018.1425402 2-s2.0-85041216467 2-s2.0-85041216467.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
212-225 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128974594768896 |