Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Detalhes bibliográficos
Autor(a) principal: Rossoni, Rodnei Dennis [UNESP]
Data de Publicação: 2018
Outros Autores: de Barros, Patrícia Pimentel [UNESP], de Alvarenga, Janaina Araújo [UNESP], Ribeiro, Felipe de Camargo [UNESP], Velloso, Marisol dos Santos [UNESP], Fuchs, Beth Burgwyn, Mylonakis, Eleftherios, Jorge, Antonio Olavo Cardoso [UNESP], Junqueira, Juliana Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/08927014.2018.1425402
http://hdl.handle.net/11449/179538
Resumo: This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.
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spelling Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasisBiofilmCandida albicansLactobacillusmycologypathogenesisThis study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.Brown UniversityFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP–Univ Estadual PaulistaDivision of Infectious Diseases Rhode Island Hospital Alpert Medical School of Brown UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP–Univ Estadual PaulistaFAPESP: 2013/25181-8FAPESP: 2013/26820-4FAPESP: 2015/09770-9Universidade Estadual Paulista (Unesp)Alpert Medical School of Brown UniversityRossoni, Rodnei Dennis [UNESP]de Barros, Patrícia Pimentel [UNESP]de Alvarenga, Janaina Araújo [UNESP]Ribeiro, Felipe de Camargo [UNESP]Velloso, Marisol dos Santos [UNESP]Fuchs, Beth BurgwynMylonakis, EleftheriosJorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]2018-12-11T17:35:35Z2018-12-11T17:35:35Z2018-02-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article212-225application/pdfhttp://dx.doi.org/10.1080/08927014.2018.1425402Biofouling, v. 34, n. 2, p. 212-225, 2018.1029-24540892-7014http://hdl.handle.net/11449/17953810.1080/08927014.2018.14254022-s2.0-850412164672-s2.0-85041216467.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2023-11-26T06:10:34Zoai:repositorio.unesp.br:11449/179538Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:45:36.032336Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
title Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
spellingShingle Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
Rossoni, Rodnei Dennis [UNESP]
Biofilm
Candida albicans
Lactobacillus
mycology
pathogenesis
title_short Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
title_full Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
title_fullStr Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
title_full_unstemmed Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
title_sort Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis
author Rossoni, Rodnei Dennis [UNESP]
author_facet Rossoni, Rodnei Dennis [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Velloso, Marisol dos Santos [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
author_role author
author2 de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Velloso, Marisol dos Santos [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Alpert Medical School of Brown University
dc.contributor.author.fl_str_mv Rossoni, Rodnei Dennis [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Velloso, Marisol dos Santos [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Jorge, Antonio Olavo Cardoso [UNESP]
Junqueira, Juliana Campos [UNESP]
dc.subject.por.fl_str_mv Biofilm
Candida albicans
Lactobacillus
mycology
pathogenesis
topic Biofilm
Candida albicans
Lactobacillus
mycology
pathogenesis
description This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:35:35Z
2018-12-11T17:35:35Z
2018-02-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/08927014.2018.1425402
Biofouling, v. 34, n. 2, p. 212-225, 2018.
1029-2454
0892-7014
http://hdl.handle.net/11449/179538
10.1080/08927014.2018.1425402
2-s2.0-85041216467
2-s2.0-85041216467.pdf
url http://dx.doi.org/10.1080/08927014.2018.1425402
http://hdl.handle.net/11449/179538
identifier_str_mv Biofouling, v. 34, n. 2, p. 212-225, 2018.
1029-2454
0892-7014
10.1080/08927014.2018.1425402
2-s2.0-85041216467
2-s2.0-85041216467.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biofouling
0,835
0,835
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 212-225
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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