Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations

Detalhes bibliográficos
Autor(a) principal: Schmitt, Fernando Carlos [UNESP]
Data de Publicação: 1995
Outros Autores: Bento, Maria José [UNESP], Amendoeira, Isabel [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/dc.2840130417
http://hdl.handle.net/11449/219176
Resumo: We describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company
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spelling Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinationsBreast cancerEstrogen receptorFNAWe describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley CompanyInstitute of Molecular Pathology and Immunology—IPATIMUP Medical School of the University of PortoCytopathology Unit Department of Pathology Hospital São João, PortoFNA Ambulatory Service Botucatu School of Medicine UNESP, BotucatuFNA Ambulatory Service Botucatu School of Medicine UNESP, BotucatuMedical School of the University of PortoHospital São JoãoUniversidade Estadual Paulista (UNESP)Schmitt, Fernando Carlos [UNESP]Bento, Maria José [UNESP]Amendoeira, Isabel [UNESP]2022-04-28T18:54:06Z2022-04-28T18:54:06Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article347-351http://dx.doi.org/10.1002/dc.2840130417Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995.1097-03398755-1039http://hdl.handle.net/11449/21917610.1002/dc.28401304172-s2.0-0028874817Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Cytopathologyinfo:eu-repo/semantics/openAccess2022-04-28T18:54:06Zoai:repositorio.unesp.br:11449/219176Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:52:04.283215Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
title Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
spellingShingle Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
Schmitt, Fernando Carlos [UNESP]
Breast cancer
Estrogen receptor
FNA
title_short Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
title_full Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
title_fullStr Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
title_full_unstemmed Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
title_sort Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
author Schmitt, Fernando Carlos [UNESP]
author_facet Schmitt, Fernando Carlos [UNESP]
Bento, Maria José [UNESP]
Amendoeira, Isabel [UNESP]
author_role author
author2 Bento, Maria José [UNESP]
Amendoeira, Isabel [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Medical School of the University of Porto
Hospital São João
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Schmitt, Fernando Carlos [UNESP]
Bento, Maria José [UNESP]
Amendoeira, Isabel [UNESP]
dc.subject.por.fl_str_mv Breast cancer
Estrogen receptor
FNA
topic Breast cancer
Estrogen receptor
FNA
description We describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company
publishDate 1995
dc.date.none.fl_str_mv 1995-01-01
2022-04-28T18:54:06Z
2022-04-28T18:54:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/dc.2840130417
Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995.
1097-0339
8755-1039
http://hdl.handle.net/11449/219176
10.1002/dc.2840130417
2-s2.0-0028874817
url http://dx.doi.org/10.1002/dc.2840130417
http://hdl.handle.net/11449/219176
identifier_str_mv Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995.
1097-0339
8755-1039
10.1002/dc.2840130417
2-s2.0-0028874817
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diagnostic Cytopathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 347-351
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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