Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/dc.2840130417 http://hdl.handle.net/11449/219176 |
Resumo: | We describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company |
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Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinationsBreast cancerEstrogen receptorFNAWe describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley CompanyInstitute of Molecular Pathology and Immunology—IPATIMUP Medical School of the University of PortoCytopathology Unit Department of Pathology Hospital São João, PortoFNA Ambulatory Service Botucatu School of Medicine UNESP, BotucatuFNA Ambulatory Service Botucatu School of Medicine UNESP, BotucatuMedical School of the University of PortoHospital São JoãoUniversidade Estadual Paulista (UNESP)Schmitt, Fernando Carlos [UNESP]Bento, Maria José [UNESP]Amendoeira, Isabel [UNESP]2022-04-28T18:54:06Z2022-04-28T18:54:06Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article347-351http://dx.doi.org/10.1002/dc.2840130417Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995.1097-03398755-1039http://hdl.handle.net/11449/21917610.1002/dc.28401304172-s2.0-0028874817Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Cytopathologyinfo:eu-repo/semantics/openAccess2022-04-28T18:54:06Zoai:repositorio.unesp.br:11449/219176Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:52:04.283215Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
title |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
spellingShingle |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations Schmitt, Fernando Carlos [UNESP] Breast cancer Estrogen receptor FNA |
title_short |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
title_full |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
title_fullStr |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
title_full_unstemmed |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
title_sort |
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations |
author |
Schmitt, Fernando Carlos [UNESP] |
author_facet |
Schmitt, Fernando Carlos [UNESP] Bento, Maria José [UNESP] Amendoeira, Isabel [UNESP] |
author_role |
author |
author2 |
Bento, Maria José [UNESP] Amendoeira, Isabel [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Medical School of the University of Porto Hospital São João Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Schmitt, Fernando Carlos [UNESP] Bento, Maria José [UNESP] Amendoeira, Isabel [UNESP] |
dc.subject.por.fl_str_mv |
Breast cancer Estrogen receptor FNA |
topic |
Breast cancer Estrogen receptor FNA |
description |
We describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-01-01 2022-04-28T18:54:06Z 2022-04-28T18:54:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/dc.2840130417 Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995. 1097-0339 8755-1039 http://hdl.handle.net/11449/219176 10.1002/dc.2840130417 2-s2.0-0028874817 |
url |
http://dx.doi.org/10.1002/dc.2840130417 http://hdl.handle.net/11449/219176 |
identifier_str_mv |
Diagnostic Cytopathology, v. 13, n. 4, p. 347-351, 1995. 1097-0339 8755-1039 10.1002/dc.2840130417 2-s2.0-0028874817 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diagnostic Cytopathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
347-351 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128869371215872 |